Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0266 and Atu0267 overlap by 4 nucleotides Atu0267 and Atu0268 are separated by 11 nucleotides Atu0268 and Atu0269 are separated by 189 nucleotides Atu0269 and Atu0270 are separated by 0 nucleotides
Atu0266: Atu0266 - oxidoreductase, at 263,264 to 264,247
Atu0266
Atu0267: Atu0267 - hypothetical protein, at 264,244 to 264,597
Atu0267
Atu0268: Atu0268 - hypothetical protein, at 264,609 to 264,851
Atu0268
Atu0269: Atu0269 - exodeoxyribonuclease VII large subunit, at 265,041 to 266,639
Atu0269
Atu0270: Atu0270 - mandelate racemase / muconate lactonizing enzyme family protein, at 266,640 to 267,842
Atu0270
Position (kb) 265
266
267 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 264.062 kb on + strand, within Atu0266 at 264.114 kb on - strand, within Atu0266 at 264.125 kb on + strand, within Atu0266 at 264.133 kb on - strand, within Atu0266 at 264.133 kb on - strand, within Atu0266 at 264.151 kb on + strand at 264.321 kb on + strand, within Atu0267 at 264.377 kb on - strand, within Atu0267 at 264.411 kb on + strand, within Atu0267 at 264.434 kb on - strand, within Atu0267 at 264.443 kb on + strand, within Atu0267 at 264.591 kb on + strand at 264.606 kb on + strand at 264.614 kb on - strand at 264.777 kb on + strand, within Atu0268 at 264.777 kb on + strand, within Atu0268 at 264.789 kb on + strand, within Atu0268 at 264.789 kb on + strand, within Atu0268 at 264.794 kb on + strand, within Atu0268 at 264.795 kb on - strand, within Atu0268 at 264.795 kb on - strand, within Atu0268 at 264.814 kb on - strand, within Atu0268 at 264.815 kb on + strand, within Atu0268 at 264.822 kb on + strand, within Atu0268 at 264.886 kb on - strand at 264.950 kb on + strand at 265.045 kb on + strand at 265.073 kb on - strand at 265.289 kb on + strand, within Atu0269 at 265.363 kb on + strand, within Atu0269 at 265.476 kb on - strand, within Atu0269 at 265.954 kb on + strand, within Atu0269 at 266.137 kb on + strand, within Atu0269 at 266.189 kb on - strand, within Atu0269 at 266.196 kb on - strand, within Atu0269 at 266.334 kb on - strand, within Atu0269 at 266.336 kb on + strand, within Atu0269 at 266.339 kb on + strand, within Atu0269 at 266.349 kb on + strand, within Atu0269 at 266.349 kb on + strand, within Atu0269 at 266.349 kb on + strand, within Atu0269 at 266.386 kb on - strand, within Atu0269 at 266.546 kb on + strand at 266.549 kb on - strand at 266.605 kb on + strand at 266.641 kb on - strand at 266.717 kb on + strand at 266.760 kb on + strand at 266.775 kb on - strand, within Atu0270 at 266.791 kb on + strand, within Atu0270 at 266.865 kb on + strand, within Atu0270 at 266.907 kb on - strand, within Atu0270 at 267.070 kb on + strand, within Atu0270 at 267.095 kb on + strand, within Atu0270 at 267.138 kb on - strand, within Atu0270 at 267.159 kb on + strand, within Atu0270 at 267.187 kb on + strand, within Atu0270 at 267.189 kb on + strand, within Atu0270 at 267.195 kb on - strand, within Atu0270 at 267.324 kb on + strand, within Atu0270 at 267.329 kb on + strand, within Atu0270 at 267.335 kb on - strand, within Atu0270 at 267.371 kb on + strand, within Atu0270 at 267.371 kb on + strand, within Atu0270 at 267.379 kb on - strand, within Atu0270 at 267.403 kb on + strand, within Atu0270 at 267.403 kb on + strand, within Atu0270 at 267.425 kb on + strand, within Atu0270 at 267.484 kb on - strand, within Atu0270 at 267.485 kb on + strand, within Atu0270 at 267.491 kb on - strand, within Atu0270
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 264,062 + Atu0266 0.81 +0.0 264,114 - Atu0266 0.86 -0.8 264,125 + Atu0266 0.88 +0.0 264,133 - Atu0266 0.88 -0.2 264,133 - Atu0266 0.88 -0.4 264,151 + +0.9 264,321 + Atu0267 0.22 -0.2 264,377 - Atu0267 0.38 +0.4 264,411 + Atu0267 0.47 -0.1 264,434 - Atu0267 0.54 +0.5 264,443 + Atu0267 0.56 -0.2 264,591 + -1.0 264,606 + +1.1 264,614 - -0.1 264,777 + Atu0268 0.69 -0.0 264,777 + Atu0268 0.69 -1.2 264,789 + Atu0268 0.74 -0.3 264,789 + Atu0268 0.74 +1.7 264,794 + Atu0268 0.76 +0.2 264,795 - Atu0268 0.77 +0.1 264,795 - Atu0268 0.77 +1.2 264,814 - Atu0268 0.84 +0.3 264,815 + Atu0268 0.85 -0.1 264,822 + Atu0268 0.88 +0.1 264,886 - +0.4 264,950 + -1.2 265,045 + -2.4 265,073 - -0.8 265,289 + Atu0269 0.16 -3.3 265,363 + Atu0269 0.20 -3.7 265,476 - Atu0269 0.27 -0.5 265,954 + Atu0269 0.57 -3.3 266,137 + Atu0269 0.69 -0.5 266,189 - Atu0269 0.72 -3.2 266,196 - Atu0269 0.72 -2.8 266,334 - Atu0269 0.81 -0.2 266,336 + Atu0269 0.81 -4.1 266,339 + Atu0269 0.81 -1.3 266,349 + Atu0269 0.82 -2.6 266,349 + Atu0269 0.82 -2.7 266,349 + Atu0269 0.82 -2.0 266,386 - Atu0269 0.84 -0.9 266,546 + +0.6 266,549 - -0.2 266,605 + -0.5 266,641 - +0.3 266,717 + +0.3 266,760 + +0.3 266,775 - Atu0270 0.11 +0.7 266,791 + Atu0270 0.13 -0.2 266,865 + Atu0270 0.19 +0.6 266,907 - Atu0270 0.22 -0.5 267,070 + Atu0270 0.36 -0.1 267,095 + Atu0270 0.38 -1.3 267,138 - Atu0270 0.41 +0.1 267,159 + Atu0270 0.43 +0.6 267,187 + Atu0270 0.45 -0.2 267,189 + Atu0270 0.46 -0.4 267,195 - Atu0270 0.46 +0.0 267,324 + Atu0270 0.57 -0.0 267,329 + Atu0270 0.57 -0.4 267,335 - Atu0270 0.58 +1.3 267,371 + Atu0270 0.61 +0.5 267,371 + Atu0270 0.61 +0.6 267,379 - Atu0270 0.61 -0.1 267,403 + Atu0270 0.63 -1.7 267,403 + Atu0270 0.63 -0.0 267,425 + Atu0270 0.65 +0.0 267,484 - Atu0270 0.70 +0.8 267,485 + Atu0270 0.70 +0.1 267,491 - Atu0270 0.71 +1.0
Or see this region's nucleotide sequence