Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0099 and Atu0100 are separated by 11 nucleotides Atu0100 and Atu0101 are separated by 150 nucleotides Atu0101 and Atu0102 are separated by 94 nucleotides
Atu0099: Atu0099 - prephenate dehydratase, at 103,016 to 103,879
Atu0099
Atu0100: Atu0100 - 3-deoxy-manno-octulosonate cytidylyltransferase, at 103,891 to 104,646
Atu0100
Atu0101: Atu0101 - cytochrome c, at 104,797 to 105,375
Atu0101
Atu0102: Atu0102 - exopolysaccharide production protein, at 105,470 to 106,195
Atu0102
Position (kb) 104
105
106 Strain fitness (log2 ratio) -2
-1
0
1
2 at 104.661 kb on - strand at 104.732 kb on - strand at 104.769 kb on - strand at 105.097 kb on - strand, within Atu0101 at 105.136 kb on + strand, within Atu0101 at 105.146 kb on + strand, within Atu0101 at 105.149 kb on + strand, within Atu0101 at 105.287 kb on + strand, within Atu0101 at 105.372 kb on - strand at 105.374 kb on - strand at 105.395 kb on - strand at 105.526 kb on - strand at 105.552 kb on + strand, within Atu0102 at 105.552 kb on + strand, within Atu0102 at 105.723 kb on + strand, within Atu0102 at 105.729 kb on - strand, within Atu0102 at 105.866 kb on - strand, within Atu0102 at 105.866 kb on - strand, within Atu0102 at 105.947 kb on + strand, within Atu0102 at 105.948 kb on - strand, within Atu0102 at 106.003 kb on - strand, within Atu0102 at 106.003 kb on - strand, within Atu0102 at 106.003 kb on - strand, within Atu0102 at 106.034 kb on - strand, within Atu0102 at 106.041 kb on - strand, within Atu0102 at 106.043 kb on + strand, within Atu0102 at 106.043 kb on + strand, within Atu0102 at 106.179 kb on - strand at 106.192 kb on + strand at 106.333 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 104,661 - -0.4 104,732 - -0.4 104,769 - +0.8 105,097 - Atu0101 0.52 -0.4 105,136 + Atu0101 0.59 -1.7 105,146 + Atu0101 0.60 +0.0 105,149 + Atu0101 0.61 -0.2 105,287 + Atu0101 0.85 -0.8 105,372 - +0.2 105,374 - +0.4 105,395 - -1.8 105,526 - +0.3 105,552 + Atu0102 0.11 +0.2 105,552 + Atu0102 0.11 -0.5 105,723 + Atu0102 0.35 +0.3 105,729 - Atu0102 0.36 -0.8 105,866 - Atu0102 0.55 -0.2 105,866 - Atu0102 0.55 +0.6 105,947 + Atu0102 0.66 -0.0 105,948 - Atu0102 0.66 +2.4 106,003 - Atu0102 0.73 +0.8 106,003 - Atu0102 0.73 -1.5 106,003 - Atu0102 0.73 +1.0 106,034 - Atu0102 0.78 +0.6 106,041 - Atu0102 0.79 -0.2 106,043 + Atu0102 0.79 +0.3 106,043 + Atu0102 0.79 +0.1 106,179 - -0.0 106,192 + -0.5 106,333 + +0.7
Or see this region's nucleotide sequence