Experiment: R2A with Nitrofurantoin 0.4 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0038 and Atu0039 are separated by 15 nucleotides Atu0039 and Atu0040 overlap by 4 nucleotides Atu0040 and Atu0041 are separated by 1 nucleotides Atu0041 and Atu8115 are separated by 1 nucleotides
Atu0038: Atu0038 - phosphoribosyl-ATP pyrophosphohydrolase, at 40,779 to 41,102
Atu0038
Atu0039: Atu0039 - imidazoleglycerol-phosphate synthase cyclase, at 41,118 to 41,894
Atu0039
Atu0040: Atu0040 - phosphoribosylformino-5-aminoimidazole carboxamide ribotide isomerase, at 41,891 to 42,634
Atu0040
Atu0041: Atu0041 - glutamine amidotransferase, at 42,636 to 43,286
Atu0041
Atu8115: Atu8115 - Hypothetical Protein, at 43,288 to 43,782
Atu8115
Position (kb) 41
42
43 Strain fitness (log2 ratio) -2
-1
0
1
2 at 41.108 kb on - strand at 41.142 kb on + strand at 41.158 kb on + strand at 41.239 kb on + strand, within Atu0039 at 41.565 kb on - strand, within Atu0039 at 41.573 kb on - strand, within Atu0039 at 41.671 kb on + strand, within Atu0039 at 41.834 kb on - strand at 41.905 kb on + strand at 41.913 kb on + strand at 42.070 kb on - strand, within Atu0040 at 42.309 kb on - strand, within Atu0040 at 42.649 kb on - strand at 42.668 kb on + strand at 42.740 kb on - strand, within Atu0041 at 42.958 kb on + strand, within Atu0041 at 42.990 kb on - strand, within Atu0041 at 43.103 kb on - strand, within Atu0041 at 43.381 kb on - strand, within Atu8115 at 43.393 kb on + strand, within Atu8115 at 43.395 kb on + strand, within Atu8115 at 43.431 kb on + strand, within Atu8115 at 43.432 kb on + strand, within Atu8115 at 43.617 kb on - strand, within Atu8115
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nitrofurantoin 0.4 mg/mL remove 41,108 - -0.6 41,142 + -1.0 41,158 + +0.8 41,239 + Atu0039 0.16 +0.4 41,565 - Atu0039 0.58 +1.0 41,573 - Atu0039 0.59 -1.8 41,671 + Atu0039 0.71 +1.0 41,834 - +1.8 41,905 + +0.7 41,913 + -1.5 42,070 - Atu0040 0.24 +0.9 42,309 - Atu0040 0.56 -1.7 42,649 - -0.2 42,668 + +1.1 42,740 - Atu0041 0.16 +0.4 42,958 + Atu0041 0.49 +0.6 42,990 - Atu0041 0.54 +0.8 43,103 - Atu0041 0.72 -1.2 43,381 - Atu8115 0.19 +1.0 43,393 + Atu8115 0.21 +0.6 43,395 + Atu8115 0.22 -1.3 43,431 + Atu8115 0.29 -0.6 43,432 + Atu8115 0.29 -0.3 43,617 - Atu8115 0.66 -0.2
Or see this region's nucleotide sequence