Experiment: R2A with Ceftazidime 4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5039 and Atu5040 are separated by 73 nucleotides Atu5040 and Atu5041 overlap by 4 nucleotides Atu5041 and Atu5042 overlap by 4 nucleotides
Atu5039: Atu5039 - hypothetical protein, at 39,240 to 40,421
Atu5039
Atu5040: Atu5040 - hypothetical protein, at 40,495 to 41,397
Atu5040
Atu5041: Atu5041 - plasmid partitioning protein, at 41,394 to 42,281
Atu5041
Atu5042: Atu5042 - recombinase, at 42,278 to 43,795
Atu5042
Position (kb) 41
42
43 Strain fitness (log2 ratio) -1
0
1
2
3 at 40.432 kb on + strand at 40.448 kb on - strand at 40.457 kb on + strand at 40.457 kb on + strand at 40.457 kb on + strand at 40.457 kb on + strand at 40.464 kb on - strand at 40.465 kb on - strand at 40.465 kb on - strand at 40.465 kb on - strand at 40.465 kb on - strand at 40.490 kb on - strand at 40.518 kb on + strand at 40.533 kb on - strand at 40.568 kb on + strand at 40.671 kb on + strand, within Atu5040 at 40.680 kb on + strand, within Atu5040 at 40.799 kb on + strand, within Atu5040 at 40.827 kb on - strand, within Atu5040 at 40.841 kb on + strand, within Atu5040 at 40.867 kb on - strand, within Atu5040 at 40.944 kb on + strand, within Atu5040 at 40.958 kb on + strand, within Atu5040 at 40.966 kb on - strand, within Atu5040 at 41.009 kb on + strand, within Atu5040 at 41.036 kb on + strand, within Atu5040 at 41.044 kb on - strand, within Atu5040 at 41.070 kb on + strand, within Atu5040 at 41.075 kb on + strand, within Atu5040 at 41.104 kb on - strand, within Atu5040 at 41.108 kb on + strand, within Atu5040 at 41.116 kb on - strand, within Atu5040 at 41.228 kb on - strand, within Atu5040 at 41.299 kb on + strand, within Atu5040 at 41.307 kb on - strand at 41.350 kb on - strand at 41.365 kb on - strand at 41.430 kb on + strand at 41.444 kb on + strand at 41.445 kb on + strand at 41.458 kb on + strand at 41.547 kb on - strand, within Atu5041 at 41.548 kb on - strand, within Atu5041 at 41.554 kb on + strand, within Atu5041 at 41.579 kb on + strand, within Atu5041 at 41.579 kb on + strand, within Atu5041 at 41.579 kb on + strand, within Atu5041 at 41.587 kb on - strand, within Atu5041 at 41.635 kb on + strand, within Atu5041 at 41.637 kb on + strand, within Atu5041 at 41.637 kb on + strand, within Atu5041 at 41.643 kb on - strand, within Atu5041 at 41.645 kb on - strand, within Atu5041 at 41.645 kb on - strand, within Atu5041 at 41.747 kb on - strand, within Atu5041 at 41.748 kb on - strand, within Atu5041 at 41.770 kb on - strand, within Atu5041 at 41.784 kb on + strand, within Atu5041 at 41.838 kb on - strand, within Atu5041 at 41.860 kb on - strand, within Atu5041 at 41.872 kb on + strand, within Atu5041 at 41.877 kb on + strand, within Atu5041 at 41.885 kb on - strand, within Atu5041 at 41.971 kb on + strand, within Atu5041 at 41.972 kb on - strand, within Atu5041 at 42.039 kb on + strand, within Atu5041 at 42.083 kb on + strand, within Atu5041 at 42.083 kb on + strand, within Atu5041 at 42.101 kb on - strand, within Atu5041 at 42.119 kb on - strand, within Atu5041 at 42.154 kb on - strand, within Atu5041 at 42.161 kb on + strand, within Atu5041 at 42.223 kb on - strand at 42.331 kb on + strand at 42.339 kb on - strand at 42.339 kb on - strand at 42.363 kb on + strand at 42.363 kb on + strand at 42.371 kb on - strand at 42.378 kb on + strand at 42.451 kb on - strand, within Atu5042 at 42.463 kb on - strand, within Atu5042 at 42.471 kb on - strand, within Atu5042 at 42.472 kb on + strand, within Atu5042 at 42.480 kb on - strand, within Atu5042 at 42.523 kb on + strand, within Atu5042 at 42.528 kb on + strand, within Atu5042 at 42.617 kb on - strand, within Atu5042 at 42.668 kb on - strand, within Atu5042 at 42.674 kb on - strand, within Atu5042 at 42.691 kb on - strand, within Atu5042 at 42.701 kb on - strand, within Atu5042 at 42.714 kb on + strand, within Atu5042 at 42.715 kb on + strand, within Atu5042 at 42.821 kb on - strand, within Atu5042 at 42.853 kb on - strand, within Atu5042 at 42.860 kb on + strand, within Atu5042 at 42.879 kb on + strand, within Atu5042 at 42.920 kb on - strand, within Atu5042 at 42.937 kb on - strand, within Atu5042 at 43.016 kb on - strand, within Atu5042 at 43.019 kb on + strand, within Atu5042 at 43.019 kb on + strand, within Atu5042 at 43.025 kb on - strand, within Atu5042 at 43.069 kb on - strand, within Atu5042 at 43.233 kb on + strand, within Atu5042
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Ceftazidime 4 mM remove 40,432 + +0.1 40,448 - -0.2 40,457 + +0.5 40,457 + -0.7 40,457 + +0.8 40,457 + +0.4 40,464 - +0.2 40,465 - +0.7 40,465 - +0.0 40,465 - -0.2 40,465 - -0.1 40,490 - +0.1 40,518 + +0.0 40,533 - +0.2 40,568 + -0.4 40,671 + Atu5040 0.19 +0.8 40,680 + Atu5040 0.20 -0.7 40,799 + Atu5040 0.34 -0.2 40,827 - Atu5040 0.37 +0.0 40,841 + Atu5040 0.38 +0.8 40,867 - Atu5040 0.41 +0.0 40,944 + Atu5040 0.50 -0.4 40,958 + Atu5040 0.51 +1.0 40,966 - Atu5040 0.52 +0.9 41,009 + Atu5040 0.57 -0.4 41,036 + Atu5040 0.60 -0.2 41,044 - Atu5040 0.61 -0.3 41,070 + Atu5040 0.64 -0.3 41,075 + Atu5040 0.64 -0.3 41,104 - Atu5040 0.67 +0.5 41,108 + Atu5040 0.68 -0.0 41,116 - Atu5040 0.69 +0.1 41,228 - Atu5040 0.81 +0.1 41,299 + Atu5040 0.89 -0.1 41,307 - -0.1 41,350 - +0.5 41,365 - -0.7 41,430 + -0.5 41,444 + +0.2 41,445 + +0.1 41,458 + -0.4 41,547 - Atu5041 0.17 +0.3 41,548 - Atu5041 0.17 +0.1 41,554 + Atu5041 0.18 +3.0 41,579 + Atu5041 0.21 +0.5 41,579 + Atu5041 0.21 +0.1 41,579 + Atu5041 0.21 -0.7 41,587 - Atu5041 0.22 +0.1 41,635 + Atu5041 0.27 +0.1 41,637 + Atu5041 0.27 -0.3 41,637 + Atu5041 0.27 -0.3 41,643 - Atu5041 0.28 -0.1 41,645 - Atu5041 0.28 +0.1 41,645 - Atu5041 0.28 +1.0 41,747 - Atu5041 0.40 -0.2 41,748 - Atu5041 0.40 -0.1 41,770 - Atu5041 0.42 +1.2 41,784 + Atu5041 0.44 +1.8 41,838 - Atu5041 0.50 -0.3 41,860 - Atu5041 0.52 -0.4 41,872 + Atu5041 0.54 -0.2 41,877 + Atu5041 0.54 -0.1 41,885 - Atu5041 0.55 -0.2 41,971 + Atu5041 0.65 +0.5 41,972 - Atu5041 0.65 +0.6 42,039 + Atu5041 0.73 +0.7 42,083 + Atu5041 0.78 -0.7 42,083 + Atu5041 0.78 -1.5 42,101 - Atu5041 0.80 +0.2 42,119 - Atu5041 0.82 -0.2 42,154 - Atu5041 0.86 -0.1 42,161 + Atu5041 0.86 -0.3 42,223 - -0.1 42,331 + -0.1 42,339 - +0.3 42,339 - +0.4 42,363 + +0.9 42,363 + -0.4 42,371 - -1.1 42,378 + -0.2 42,451 - Atu5042 0.11 -0.5 42,463 - Atu5042 0.12 +1.3 42,471 - Atu5042 0.13 +1.0 42,472 + Atu5042 0.13 +1.0 42,480 - Atu5042 0.13 +0.6 42,523 + Atu5042 0.16 +0.6 42,528 + Atu5042 0.16 -0.6 42,617 - Atu5042 0.22 -0.3 42,668 - Atu5042 0.26 +0.2 42,674 - Atu5042 0.26 -0.4 42,691 - Atu5042 0.27 +0.5 42,701 - Atu5042 0.28 -0.4 42,714 + Atu5042 0.29 +1.5 42,715 + Atu5042 0.29 +0.3 42,821 - Atu5042 0.36 +0.0 42,853 - Atu5042 0.38 -0.7 42,860 + Atu5042 0.38 +0.1 42,879 + Atu5042 0.40 -0.3 42,920 - Atu5042 0.42 -0.1 42,937 - Atu5042 0.43 +1.8 43,016 - Atu5042 0.49 +0.5 43,019 + Atu5042 0.49 -0.6 43,019 + Atu5042 0.49 +0.1 43,025 - Atu5042 0.49 +0.4 43,069 - Atu5042 0.52 -1.5 43,233 + Atu5042 0.63 +0.3
Or see this region's nucleotide sequence