Experiment: R2A with Ceftazidime 4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5010 and Atu5011 are separated by 364 nucleotides
Atu5010: Atu5010 - hypothetical protein, at 12,517 to 12,717
Atu5010
Atu5011: Atu5011 - polyketide biosynthesis dithiol-disulfide isomerase, at 13,082 to 13,723
Atu5011
Position (kb)
12
13 Strain fitness (log2 ratio)
-1
0
1 at 11.685 kb on + strand at 11.700 kb on - strand at 11.751 kb on - strand at 11.832 kb on - strand at 11.832 kb on - strand at 11.842 kb on + strand at 11.843 kb on + strand at 11.850 kb on - strand at 11.869 kb on - strand at 11.935 kb on + strand at 12.024 kb on + strand at 12.025 kb on + strand at 12.047 kb on + strand at 12.055 kb on - strand at 12.055 kb on - strand at 12.055 kb on - strand at 12.055 kb on - strand at 12.055 kb on - strand at 12.060 kb on - strand at 12.241 kb on - strand at 12.248 kb on + strand at 12.263 kb on + strand at 12.306 kb on + strand at 12.404 kb on - strand at 12.434 kb on + strand at 12.496 kb on + strand at 12.515 kb on - strand at 12.591 kb on - strand, within Atu5010 at 12.637 kb on + strand, within Atu5010 at 12.682 kb on + strand, within Atu5010 at 12.682 kb on + strand, within Atu5010 at 12.746 kb on + strand at 12.827 kb on + strand at 12.827 kb on + strand at 12.835 kb on - strand at 12.932 kb on - strand at 12.980 kb on - strand at 13.116 kb on + strand at 13.175 kb on + strand, within Atu5011 at 13.207 kb on - strand, within Atu5011 at 13.224 kb on + strand, within Atu5011 at 13.280 kb on + strand, within Atu5011 at 13.390 kb on + strand, within Atu5011 at 13.569 kb on - strand, within Atu5011 at 13.569 kb on - strand, within Atu5011 at 13.574 kb on + strand, within Atu5011 at 13.619 kb on - strand, within Atu5011
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Ceftazidime 4 mM remove 11,685 + +0.3 11,700 - -0.2 11,751 - +1.6 11,832 - -0.5 11,832 - -0.2 11,842 + -0.2 11,843 + -0.1 11,850 - +0.2 11,869 - +0.3 11,935 + -0.2 12,024 + +0.3 12,025 + +0.1 12,047 + +0.1 12,055 - -0.5 12,055 - +1.0 12,055 - +0.3 12,055 - -1.0 12,055 - +0.4 12,060 - +1.2 12,241 - +0.4 12,248 + -0.3 12,263 + +0.1 12,306 + +0.2 12,404 - +0.2 12,434 + +1.0 12,496 + -0.3 12,515 - +0.2 12,591 - Atu5010 0.37 -1.0 12,637 + Atu5010 0.60 -0.8 12,682 + Atu5010 0.82 -0.4 12,682 + Atu5010 0.82 +0.2 12,746 + -1.1 12,827 + +0.2 12,827 + +0.4 12,835 - -0.3 12,932 - -0.1 12,980 - +1.5 13,116 + +0.6 13,175 + Atu5011 0.14 +0.4 13,207 - Atu5011 0.19 +0.7 13,224 + Atu5011 0.22 +1.3 13,280 + Atu5011 0.31 -0.1 13,390 + Atu5011 0.48 +0.0 13,569 - Atu5011 0.76 +0.3 13,569 - Atu5011 0.76 -0.7 13,574 + Atu5011 0.77 -0.1 13,619 - Atu5011 0.84 -0.0
Or see this region's nucleotide sequence