Experiment: R2A with Ceftazidime 4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4280 and Atu4281 overlap by 4 nucleotides Atu4281 and Atu4282 overlap by 4 nucleotides Atu4282 and Atu4283 overlap by 4 nucleotides
Atu4280: Atu4280 - short-chain dehydrogenase, at 1,405,144 to 1,405,923
Atu4280
Atu4281: Atu4281 - amino acid ABC transporter permease, at 1,405,920 to 1,406,627
Atu4281
Atu4282: Atu4282 - amino acid ABC transporter permease, at 1,406,624 to 1,407,382
Atu4282
Atu4283: Atu4283 - ABC transporter permease, at 1,407,379 to 1,408,143
Atu4283
Position (kb)
1405
1406
1407 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1404.948 kb on - strand at 1404.995 kb on + strand at 1405.094 kb on - strand at 1405.234 kb on + strand, within Atu4280 at 1405.290 kb on + strand, within Atu4280 at 1405.448 kb on + strand, within Atu4280 at 1405.448 kb on + strand, within Atu4280 at 1405.456 kb on - strand, within Atu4280 at 1405.489 kb on + strand, within Atu4280 at 1405.712 kb on - strand, within Atu4280 at 1405.848 kb on + strand at 1405.875 kb on + strand at 1405.875 kb on + strand at 1405.883 kb on - strand at 1405.883 kb on - strand at 1405.891 kb on - strand at 1405.896 kb on - strand at 1405.922 kb on - strand at 1405.927 kb on + strand at 1405.935 kb on - strand at 1405.944 kb on + strand at 1405.984 kb on + strand at 1405.989 kb on + strand at 1405.990 kb on + strand at 1405.999 kb on + strand, within Atu4281 at 1406.156 kb on - strand, within Atu4281 at 1406.182 kb on - strand, within Atu4281 at 1406.239 kb on + strand, within Atu4281 at 1406.249 kb on + strand, within Atu4281 at 1406.249 kb on + strand, within Atu4281 at 1406.249 kb on + strand, within Atu4281 at 1406.257 kb on - strand, within Atu4281 at 1406.302 kb on + strand, within Atu4281 at 1406.307 kb on + strand, within Atu4281 at 1406.310 kb on - strand, within Atu4281 at 1406.310 kb on - strand, within Atu4281 at 1406.450 kb on + strand, within Atu4281 at 1406.538 kb on + strand, within Atu4281 at 1406.546 kb on - strand, within Atu4281 at 1406.594 kb on - strand at 1406.606 kb on + strand at 1406.606 kb on + strand at 1406.606 kb on + strand at 1406.607 kb on + strand at 1406.614 kb on - strand at 1406.640 kb on + strand at 1406.661 kb on - strand at 1406.827 kb on + strand, within Atu4282 at 1406.835 kb on - strand, within Atu4282 at 1406.835 kb on - strand, within Atu4282 at 1406.835 kb on - strand, within Atu4282 at 1406.867 kb on - strand, within Atu4282 at 1406.890 kb on + strand, within Atu4282 at 1406.909 kb on - strand, within Atu4282 at 1406.948 kb on + strand, within Atu4282 at 1406.982 kb on - strand, within Atu4282 at 1407.008 kb on - strand, within Atu4282 at 1407.087 kb on + strand, within Atu4282 at 1407.137 kb on + strand, within Atu4282 at 1407.164 kb on + strand, within Atu4282 at 1407.172 kb on - strand, within Atu4282 at 1407.172 kb on - strand, within Atu4282 at 1407.172 kb on - strand, within Atu4282 at 1407.239 kb on - strand, within Atu4282 at 1407.246 kb on + strand, within Atu4282 at 1407.254 kb on - strand, within Atu4282 at 1407.344 kb on + strand at 1407.457 kb on - strand, within Atu4283 at 1407.495 kb on + strand, within Atu4283
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Ceftazidime 4 mM remove 1,404,948 - +0.2 1,404,995 + -0.0 1,405,094 - +0.5 1,405,234 + Atu4280 0.12 +0.3 1,405,290 + Atu4280 0.19 -2.0 1,405,448 + Atu4280 0.39 -0.3 1,405,448 + Atu4280 0.39 +0.2 1,405,456 - Atu4280 0.40 +0.1 1,405,489 + Atu4280 0.44 -0.0 1,405,712 - Atu4280 0.73 -0.0 1,405,848 + -0.4 1,405,875 + -1.1 1,405,875 + -0.1 1,405,883 - +0.1 1,405,883 - +0.7 1,405,891 - +0.3 1,405,896 - -0.3 1,405,922 - +1.0 1,405,927 + -0.2 1,405,935 - -0.2 1,405,944 + +0.6 1,405,984 + -0.1 1,405,989 + -0.2 1,405,990 + -0.8 1,405,999 + Atu4281 0.11 +0.7 1,406,156 - Atu4281 0.33 +0.0 1,406,182 - Atu4281 0.37 +0.0 1,406,239 + Atu4281 0.45 -0.2 1,406,249 + Atu4281 0.46 -0.0 1,406,249 + Atu4281 0.46 +0.1 1,406,249 + Atu4281 0.46 +0.4 1,406,257 - Atu4281 0.48 -0.4 1,406,302 + Atu4281 0.54 +0.9 1,406,307 + Atu4281 0.55 +0.2 1,406,310 - Atu4281 0.55 +0.6 1,406,310 - Atu4281 0.55 -0.1 1,406,450 + Atu4281 0.75 +0.0 1,406,538 + Atu4281 0.87 +0.2 1,406,546 - Atu4281 0.88 +0.0 1,406,594 - +0.3 1,406,606 + +0.2 1,406,606 + -0.8 1,406,606 + +0.1 1,406,607 + +1.1 1,406,614 - +0.0 1,406,640 + -0.6 1,406,661 - +0.2 1,406,827 + Atu4282 0.27 -0.2 1,406,835 - Atu4282 0.28 -0.1 1,406,835 - Atu4282 0.28 -0.3 1,406,835 - Atu4282 0.28 +0.7 1,406,867 - Atu4282 0.32 +0.7 1,406,890 + Atu4282 0.35 +0.6 1,406,909 - Atu4282 0.38 +1.8 1,406,948 + Atu4282 0.43 +1.5 1,406,982 - Atu4282 0.47 +0.1 1,407,008 - Atu4282 0.51 +0.5 1,407,087 + Atu4282 0.61 -0.7 1,407,137 + Atu4282 0.68 +0.9 1,407,164 + Atu4282 0.71 +0.0 1,407,172 - Atu4282 0.72 -0.4 1,407,172 - Atu4282 0.72 +1.6 1,407,172 - Atu4282 0.72 -0.1 1,407,239 - Atu4282 0.81 -0.3 1,407,246 + Atu4282 0.82 +1.2 1,407,254 - Atu4282 0.83 -0.1 1,407,344 + -0.5 1,407,457 - Atu4283 0.10 +0.5 1,407,495 + Atu4283 0.15 -0.1
Or see this region's nucleotide sequence