Experiment: R2A with Ceftazidime 4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1050 and Atu1051 are separated by 77 nucleotides Atu1051 and Atu1052 overlap by 4 nucleotides Atu1052 and Atu1053 are separated by 38 nucleotides
Atu1050: Atu1050 - hypothetical protein, at 1,043,852 to 1,044,673
Atu1050
Atu1051: Atu1051 - hypothetical protein, at 1,044,751 to 1,045,269
Atu1051
Atu1052: Atu1052 - hypothetical protein, at 1,045,266 to 1,045,853
Atu1052
Atu1053: Atu1053 - glycine cleavage system T protein, aminomethyltransferase, at 1,045,892 to 1,046,740
Atu1053
Position (kb)
1045
1046 Strain fitness (log2 ratio)
-1
0
1 at 1044.268 kb on - strand, within Atu1050 at 1044.268 kb on - strand, within Atu1050 at 1044.288 kb on - strand, within Atu1050 at 1044.331 kb on - strand, within Atu1050 at 1044.331 kb on - strand, within Atu1050 at 1044.343 kb on + strand, within Atu1050 at 1044.351 kb on - strand, within Atu1050 at 1044.429 kb on - strand, within Atu1050 at 1044.429 kb on - strand, within Atu1050 at 1044.674 kb on + strand at 1045.027 kb on - strand, within Atu1051 at 1045.588 kb on + strand, within Atu1052 at 1045.596 kb on - strand, within Atu1052 at 1045.732 kb on - strand, within Atu1052 at 1045.739 kb on - strand, within Atu1052 at 1045.787 kb on - strand, within Atu1052 at 1045.814 kb on + strand at 1045.827 kb on - strand at 1045.890 kb on + strand at 1046.160 kb on - strand, within Atu1053 at 1046.766 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Ceftazidime 4 mM remove 1,044,268 - Atu1050 0.51 -0.4 1,044,268 - Atu1050 0.51 +0.0 1,044,288 - Atu1050 0.53 +0.2 1,044,331 - Atu1050 0.58 -0.2 1,044,331 - Atu1050 0.58 -0.0 1,044,343 + Atu1050 0.60 -0.0 1,044,351 - Atu1050 0.61 -0.5 1,044,429 - Atu1050 0.70 -0.8 1,044,429 - Atu1050 0.70 -0.1 1,044,674 + +0.5 1,045,027 - Atu1051 0.53 -0.4 1,045,588 + Atu1052 0.55 +0.3 1,045,596 - Atu1052 0.56 -0.2 1,045,732 - Atu1052 0.79 +0.1 1,045,739 - Atu1052 0.80 -0.9 1,045,787 - Atu1052 0.89 -1.0 1,045,814 + -0.6 1,045,827 - -0.2 1,045,890 + -0.2 1,046,160 - Atu1053 0.32 -0.4 1,046,766 - -0.0
Or see this region's nucleotide sequence