Experiment: R2A with Ceftazidime 4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0233 and Atu0234 are separated by 3 nucleotides Atu0234 and Atu0235 are separated by 131 nucleotides
Atu0233: Atu0233 - lysozyme, at 233,224 to 234,321
Atu0233
Atu0234: Atu0234 - conserved hypothetical protein, membrane protein, at 234,325 to 235,200
Atu0234
Atu0235: Atu0235 - transcriptional regulator, GntR family, at 235,332 to 236,705
Atu0235
Position (kb)
234
235
236 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 233.388 kb on + strand, within Atu0233 at 233.429 kb on - strand, within Atu0233 at 233.432 kb on + strand, within Atu0233 at 233.448 kb on + strand, within Atu0233 at 233.486 kb on - strand, within Atu0233 at 233.619 kb on + strand, within Atu0233 at 233.654 kb on + strand, within Atu0233 at 233.708 kb on + strand, within Atu0233 at 233.708 kb on + strand, within Atu0233 at 233.708 kb on - strand, within Atu0233 at 233.716 kb on - strand, within Atu0233 at 233.730 kb on + strand, within Atu0233 at 233.734 kb on + strand, within Atu0233 at 233.734 kb on + strand, within Atu0233 at 233.742 kb on - strand, within Atu0233 at 233.752 kb on - strand, within Atu0233 at 233.830 kb on + strand, within Atu0233 at 233.897 kb on - strand, within Atu0233 at 233.999 kb on + strand, within Atu0233 at 234.063 kb on + strand, within Atu0233 at 234.063 kb on + strand, within Atu0233 at 234.169 kb on - strand, within Atu0233 at 234.181 kb on + strand, within Atu0233 at 234.366 kb on - strand at 234.374 kb on - strand at 234.390 kb on - strand at 234.426 kb on + strand, within Atu0234 at 234.443 kb on + strand, within Atu0234 at 234.489 kb on - strand, within Atu0234 at 234.669 kb on + strand, within Atu0234 at 234.689 kb on - strand, within Atu0234 at 234.939 kb on + strand, within Atu0234 at 234.957 kb on + strand, within Atu0234 at 235.023 kb on + strand, within Atu0234 at 235.203 kb on - strand at 235.214 kb on - strand at 235.328 kb on + strand at 235.388 kb on + strand at 235.393 kb on + strand at 235.393 kb on + strand at 235.401 kb on - strand at 235.401 kb on - strand at 235.401 kb on - strand at 235.401 kb on - strand at 235.433 kb on + strand at 235.433 kb on + strand at 235.441 kb on - strand at 235.441 kb on - strand at 235.692 kb on + strand, within Atu0235 at 235.783 kb on + strand, within Atu0235 at 235.811 kb on - strand, within Atu0235 at 235.813 kb on + strand, within Atu0235 at 235.813 kb on + strand, within Atu0235 at 235.813 kb on + strand, within Atu0235 at 235.813 kb on + strand, within Atu0235 at 235.815 kb on + strand, within Atu0235 at 235.815 kb on + strand, within Atu0235 at 235.821 kb on - strand, within Atu0235 at 235.821 kb on - strand, within Atu0235 at 235.821 kb on - strand, within Atu0235 at 235.823 kb on - strand, within Atu0235 at 235.825 kb on + strand, within Atu0235 at 235.949 kb on + strand, within Atu0235 at 235.980 kb on + strand, within Atu0235 at 235.982 kb on + strand, within Atu0235 at 235.982 kb on + strand, within Atu0235 at 235.982 kb on + strand, within Atu0235 at 235.984 kb on + strand, within Atu0235 at 235.993 kb on - strand, within Atu0235 at 235.993 kb on - strand, within Atu0235 at 236.086 kb on + strand, within Atu0235 at 236.094 kb on - strand, within Atu0235 at 236.142 kb on + strand, within Atu0235 at 236.150 kb on - strand, within Atu0235 at 236.153 kb on + strand, within Atu0235
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Ceftazidime 4 mM remove 233,388 + Atu0233 0.15 +3.5 233,429 - Atu0233 0.19 +3.3 233,432 + Atu0233 0.19 +3.4 233,448 + Atu0233 0.20 +3.3 233,486 - Atu0233 0.24 +3.3 233,619 + Atu0233 0.36 +3.3 233,654 + Atu0233 0.39 +3.6 233,708 + Atu0233 0.44 +3.1 233,708 + Atu0233 0.44 +3.8 233,708 - Atu0233 0.44 +3.2 233,716 - Atu0233 0.45 +3.6 233,730 + Atu0233 0.46 +3.3 233,734 + Atu0233 0.46 +4.2 233,734 + Atu0233 0.46 +3.0 233,742 - Atu0233 0.47 +3.3 233,752 - Atu0233 0.48 +3.4 233,830 + Atu0233 0.55 +3.5 233,897 - Atu0233 0.61 +3.5 233,999 + Atu0233 0.71 +3.5 234,063 + Atu0233 0.76 +3.3 234,063 + Atu0233 0.76 +3.7 234,169 - Atu0233 0.86 +2.5 234,181 + Atu0233 0.87 +3.3 234,366 - -0.4 234,374 - -0.1 234,390 - -0.4 234,426 + Atu0234 0.12 +0.2 234,443 + Atu0234 0.13 -0.3 234,489 - Atu0234 0.19 -0.3 234,669 + Atu0234 0.39 +0.2 234,689 - Atu0234 0.42 +0.2 234,939 + Atu0234 0.70 -0.7 234,957 + Atu0234 0.72 -0.8 235,023 + Atu0234 0.80 +0.2 235,203 - +0.4 235,214 - -0.7 235,328 + +0.5 235,388 + -0.4 235,393 + -0.3 235,393 + +0.4 235,401 - +0.6 235,401 - +0.8 235,401 - -0.4 235,401 - -0.4 235,433 + +0.7 235,433 + +0.9 235,441 - +0.0 235,441 - -0.6 235,692 + Atu0235 0.26 -0.4 235,783 + Atu0235 0.33 -0.4 235,811 - Atu0235 0.35 +0.5 235,813 + Atu0235 0.35 -0.0 235,813 + Atu0235 0.35 +0.3 235,813 + Atu0235 0.35 +0.3 235,813 + Atu0235 0.35 +0.5 235,815 + Atu0235 0.35 +0.0 235,815 + Atu0235 0.35 -0.1 235,821 - Atu0235 0.36 +0.4 235,821 - Atu0235 0.36 +0.0 235,821 - Atu0235 0.36 +0.6 235,823 - Atu0235 0.36 +0.2 235,825 + Atu0235 0.36 -0.1 235,949 + Atu0235 0.45 -0.5 235,980 + Atu0235 0.47 +0.2 235,982 + Atu0235 0.47 +0.0 235,982 + Atu0235 0.47 -0.7 235,982 + Atu0235 0.47 -0.3 235,984 + Atu0235 0.47 +0.5 235,993 - Atu0235 0.48 +0.1 235,993 - Atu0235 0.48 -1.5 236,086 + Atu0235 0.55 -0.3 236,094 - Atu0235 0.55 +0.4 236,142 + Atu0235 0.59 -0.1 236,150 - Atu0235 0.60 -0.4 236,153 + Atu0235 0.60 -0.2
Or see this region's nucleotide sequence