Experiment: R2A with Ceftazidime 4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0229 and Atu0230 are separated by 3 nucleotides Atu0230 and Atu0231 overlap by 2 nucleotides Atu0231 and Atu0232 are separated by 87 nucleotides Atu0232 and Atu0233 are separated by 182 nucleotides
Atu0229: Atu0229 - hypothetical protein, at 231,481 to 231,930
Atu0229
Atu0230: Atu0230 - hypothetical protein, at 231,934 to 232,092
Atu0230
Atu0231: Atu0231 - GMP synthase, at 232,091 to 232,765
Atu0231
Atu0232: Atu0232 - hypothetical protein, at 232,853 to 233,041
Atu0232
Atu0233: Atu0233 - lysozyme, at 233,224 to 234,321
Atu0233
Position (kb) 232
233
234 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 231.899 kb on - strand at 231.940 kb on - strand at 232.010 kb on - strand, within Atu0230 at 232.124 kb on + strand at 232.165 kb on - strand, within Atu0231 at 232.252 kb on + strand, within Atu0231 at 232.273 kb on - strand, within Atu0231 at 232.273 kb on - strand, within Atu0231 at 232.293 kb on - strand, within Atu0231 at 232.306 kb on + strand, within Atu0231 at 232.331 kb on - strand, within Atu0231 at 232.334 kb on + strand, within Atu0231 at 232.401 kb on + strand, within Atu0231 at 232.434 kb on - strand, within Atu0231 at 232.434 kb on - strand, within Atu0231 at 232.541 kb on + strand, within Atu0231 at 232.727 kb on + strand at 232.744 kb on + strand at 232.884 kb on - strand, within Atu0232 at 232.902 kb on + strand, within Atu0232 at 232.952 kb on - strand, within Atu0232 at 232.953 kb on - strand, within Atu0232 at 232.953 kb on - strand, within Atu0232 at 232.953 kb on - strand, within Atu0232 at 232.965 kb on - strand, within Atu0232 at 232.965 kb on - strand, within Atu0232 at 233.013 kb on + strand, within Atu0232 at 233.021 kb on - strand, within Atu0232 at 233.200 kb on - strand at 233.210 kb on + strand at 233.258 kb on - strand at 233.388 kb on + strand, within Atu0233 at 233.429 kb on - strand, within Atu0233 at 233.432 kb on + strand, within Atu0233 at 233.448 kb on + strand, within Atu0233 at 233.486 kb on - strand, within Atu0233 at 233.619 kb on + strand, within Atu0233 at 233.654 kb on + strand, within Atu0233 at 233.708 kb on + strand, within Atu0233 at 233.708 kb on + strand, within Atu0233 at 233.708 kb on - strand, within Atu0233 at 233.716 kb on - strand, within Atu0233 at 233.730 kb on + strand, within Atu0233 at 233.734 kb on + strand, within Atu0233 at 233.734 kb on + strand, within Atu0233 at 233.742 kb on - strand, within Atu0233 at 233.752 kb on - strand, within Atu0233 at 233.830 kb on + strand, within Atu0233 at 233.897 kb on - strand, within Atu0233 at 233.999 kb on + strand, within Atu0233
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Ceftazidime 4 mM remove 231,899 - +0.2 231,940 - +0.9 232,010 - Atu0230 0.48 +0.2 232,124 + +0.2 232,165 - Atu0231 0.11 +0.7 232,252 + Atu0231 0.24 -0.1 232,273 - Atu0231 0.27 +0.6 232,273 - Atu0231 0.27 +0.5 232,293 - Atu0231 0.30 +0.1 232,306 + Atu0231 0.32 -0.1 232,331 - Atu0231 0.36 +0.5 232,334 + Atu0231 0.36 -0.6 232,401 + Atu0231 0.46 +0.2 232,434 - Atu0231 0.51 +0.1 232,434 - Atu0231 0.51 -0.1 232,541 + Atu0231 0.67 -0.0 232,727 + +0.2 232,744 + -0.0 232,884 - Atu0232 0.16 +0.5 232,902 + Atu0232 0.26 -0.4 232,952 - Atu0232 0.52 +0.9 232,953 - Atu0232 0.53 +0.4 232,953 - Atu0232 0.53 +0.2 232,953 - Atu0232 0.53 -0.5 232,965 - Atu0232 0.59 +0.1 232,965 - Atu0232 0.59 -0.3 233,013 + Atu0232 0.85 +0.1 233,021 - Atu0232 0.89 +0.0 233,200 - +2.4 233,210 + +2.5 233,258 - +3.7 233,388 + Atu0233 0.15 +3.5 233,429 - Atu0233 0.19 +3.3 233,432 + Atu0233 0.19 +3.4 233,448 + Atu0233 0.20 +3.3 233,486 - Atu0233 0.24 +3.3 233,619 + Atu0233 0.36 +3.3 233,654 + Atu0233 0.39 +3.6 233,708 + Atu0233 0.44 +3.1 233,708 + Atu0233 0.44 +3.8 233,708 - Atu0233 0.44 +3.2 233,716 - Atu0233 0.45 +3.6 233,730 + Atu0233 0.46 +3.3 233,734 + Atu0233 0.46 +4.2 233,734 + Atu0233 0.46 +3.0 233,742 - Atu0233 0.47 +3.3 233,752 - Atu0233 0.48 +3.4 233,830 + Atu0233 0.55 +3.5 233,897 - Atu0233 0.61 +3.5 233,999 + Atu0233 0.71 +3.5
Or see this region's nucleotide sequence