Experiment: R2A with Azlocillin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3274 and Atu3275 overlap by 4 nucleotides Atu3275 and Atu3276 overlap by 1 nucleotides
Atu3274: Atu3274 - AcrB/AcrD/AcrF family protein, at 294,591 to 297,731
Atu3274
Atu3275: Atu3275 - invasion associated protein B, at 297,728 to 298,306
Atu3275
Atu3276: Atu3276 - hypothetical protein, at 298,306 to 302,331
Atu3276
Position (kb) 297
298
299 Strain fitness (log2 ratio) -1
0
1 at 296.767 kb on - strand, within Atu3274 at 296.913 kb on + strand, within Atu3274 at 296.913 kb on + strand, within Atu3274 at 296.948 kb on - strand, within Atu3274 at 296.990 kb on - strand, within Atu3274 at 297.015 kb on - strand, within Atu3274 at 297.025 kb on - strand, within Atu3274 at 297.052 kb on + strand, within Atu3274 at 297.070 kb on - strand, within Atu3274 at 297.070 kb on - strand, within Atu3274 at 297.130 kb on + strand, within Atu3274 at 297.340 kb on - strand, within Atu3274 at 297.383 kb on - strand, within Atu3274 at 297.494 kb on - strand at 297.517 kb on + strand at 297.517 kb on + strand at 297.597 kb on + strand at 297.640 kb on - strand at 297.671 kb on + strand at 297.676 kb on + strand at 297.801 kb on - strand, within Atu3275 at 297.832 kb on - strand, within Atu3275 at 297.880 kb on + strand, within Atu3275 at 297.901 kb on + strand, within Atu3275 at 297.906 kb on + strand, within Atu3275 at 297.914 kb on - strand, within Atu3275 at 297.919 kb on - strand, within Atu3275 at 297.956 kb on - strand, within Atu3275 at 298.005 kb on - strand, within Atu3275 at 298.005 kb on - strand, within Atu3275 at 298.082 kb on - strand, within Atu3275 at 298.174 kb on - strand, within Atu3275 at 298.242 kb on - strand, within Atu3275 at 298.337 kb on + strand at 298.404 kb on + strand at 298.406 kb on + strand at 298.424 kb on - strand at 298.454 kb on - strand at 298.504 kb on - strand at 298.529 kb on + strand at 298.626 kb on + strand at 298.665 kb on + strand at 298.698 kb on - strand at 298.724 kb on - strand, within Atu3276 at 298.757 kb on + strand, within Atu3276 at 298.774 kb on + strand, within Atu3276 at 298.811 kb on - strand, within Atu3276 at 298.922 kb on - strand, within Atu3276 at 298.922 kb on - strand, within Atu3276 at 298.956 kb on + strand, within Atu3276 at 298.956 kb on + strand, within Atu3276 at 298.972 kb on + strand, within Atu3276 at 299.078 kb on + strand, within Atu3276 at 299.157 kb on - strand, within Atu3276 at 299.205 kb on + strand, within Atu3276 at 299.243 kb on - strand, within Atu3276 at 299.268 kb on - strand, within Atu3276
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Azlocillin 0.2 mM remove 296,767 - Atu3274 0.69 -1.3 296,913 + Atu3274 0.74 -0.5 296,913 + Atu3274 0.74 +0.5 296,948 - Atu3274 0.75 -0.3 296,990 - Atu3274 0.76 +0.1 297,015 - Atu3274 0.77 +1.3 297,025 - Atu3274 0.77 -0.1 297,052 + Atu3274 0.78 +0.6 297,070 - Atu3274 0.79 +0.3 297,070 - Atu3274 0.79 -0.2 297,130 + Atu3274 0.81 -0.0 297,340 - Atu3274 0.88 +0.1 297,383 - Atu3274 0.89 -0.1 297,494 - -0.6 297,517 + +0.5 297,517 + -0.1 297,597 + -0.3 297,640 - -1.4 297,671 + -0.4 297,676 + -0.0 297,801 - Atu3275 0.13 +0.1 297,832 - Atu3275 0.18 +0.4 297,880 + Atu3275 0.26 +1.6 297,901 + Atu3275 0.30 -0.2 297,906 + Atu3275 0.31 -0.3 297,914 - Atu3275 0.32 +1.4 297,919 - Atu3275 0.33 +0.2 297,956 - Atu3275 0.39 -0.5 298,005 - Atu3275 0.48 +0.5 298,005 - Atu3275 0.48 +0.1 298,082 - Atu3275 0.61 +0.8 298,174 - Atu3275 0.77 -0.0 298,242 - Atu3275 0.89 +0.7 298,337 + -0.1 298,404 + -0.3 298,406 + +0.2 298,424 - -0.2 298,454 - +0.5 298,504 - +0.6 298,529 + -0.4 298,626 + +0.6 298,665 + -0.1 298,698 - -0.1 298,724 - Atu3276 0.10 -0.3 298,757 + Atu3276 0.11 -0.2 298,774 + Atu3276 0.12 -0.6 298,811 - Atu3276 0.13 -0.3 298,922 - Atu3276 0.15 +0.4 298,922 - Atu3276 0.15 +0.0 298,956 + Atu3276 0.16 -0.5 298,956 + Atu3276 0.16 -0.1 298,972 + Atu3276 0.17 -0.8 299,078 + Atu3276 0.19 -0.3 299,157 - Atu3276 0.21 -0.1 299,205 + Atu3276 0.22 -0.1 299,243 - Atu3276 0.23 -0.2 299,268 - Atu3276 0.24 -0.7
Or see this region's nucleotide sequence