Experiment: R2A with Azlocillin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1721 and Atu1722 are separated by 184 nucleotides Atu1722 and Atu1723 are separated by 89 nucleotides Atu1723 and Atu1724 are separated by 702 nucleotides
Atu1721: Atu1721 - protein-L-isoaspartate O-methyltransferase, at 1,709,941 to 1,710,612
Atu1721
Atu1722: Atu1722 - hypothetical protein, at 1,710,797 to 1,710,991
Atu1722
Atu1723: Atu1723 - tRNA-Cys, at 1,711,081 to 1,711,154
Atu1723
Atu1724: Atu1724 - hypothetical protein, at 1,711,857 to 1,712,057
Atu1724
Position (kb)
1710
1711 Strain fitness (log2 ratio)
-2
-1
0
1 at 1709.984 kb on - strand at 1710.741 kb on + strand at 1710.849 kb on + strand, within Atu1722 at 1710.849 kb on + strand, within Atu1722 at 1710.886 kb on + strand, within Atu1722 at 1710.904 kb on - strand, within Atu1722 at 1710.946 kb on + strand, within Atu1722 at 1710.948 kb on - strand, within Atu1722 at 1711.003 kb on - strand at 1711.345 kb on - strand at 1711.371 kb on + strand at 1711.380 kb on - strand at 1711.411 kb on + strand at 1711.419 kb on - strand at 1711.419 kb on - strand at 1711.426 kb on - strand at 1711.430 kb on + strand at 1711.438 kb on - strand at 1711.441 kb on + strand at 1711.492 kb on + strand at 1711.531 kb on + strand at 1711.531 kb on + strand at 1711.539 kb on - strand at 1711.555 kb on - strand at 1711.560 kb on - strand at 1711.580 kb on - strand at 1711.676 kb on - strand at 1711.793 kb on - strand at 1711.804 kb on + strand at 1711.839 kb on + strand at 1711.952 kb on - strand, within Atu1724 at 1711.970 kb on - strand, within Atu1724 at 1711.989 kb on - strand, within Atu1724
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Azlocillin 0.2 mM remove 1,709,984 - -0.1 1,710,741 + +0.2 1,710,849 + Atu1722 0.27 +0.4 1,710,849 + Atu1722 0.27 +1.0 1,710,886 + Atu1722 0.46 +0.5 1,710,904 - Atu1722 0.55 +0.9 1,710,946 + Atu1722 0.76 +0.5 1,710,948 - Atu1722 0.77 -0.5 1,711,003 - -0.2 1,711,345 - -0.1 1,711,371 + -0.1 1,711,380 - +0.3 1,711,411 + +0.4 1,711,419 - -0.3 1,711,419 - +0.7 1,711,426 - +0.0 1,711,430 + -0.1 1,711,438 - -0.5 1,711,441 + -0.1 1,711,492 + -0.2 1,711,531 + +0.3 1,711,531 + +0.4 1,711,539 - +1.6 1,711,555 - -0.2 1,711,560 - -0.2 1,711,580 - -0.3 1,711,676 - +0.5 1,711,793 - +0.3 1,711,804 + -0.0 1,711,839 + -2.0 1,711,952 - Atu1724 0.47 -0.2 1,711,970 - Atu1724 0.56 +1.3 1,711,989 - Atu1724 0.66 +0.4
Or see this region's nucleotide sequence