Experiment: R2A with Azlocillin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1448 and Atu1449 are separated by 48 nucleotides Atu1449 and Atu1450 are separated by 184 nucleotides Atu1450 and Atu1451 are separated by 206 nucleotides
Atu1448: Atu1448 - two component response regulator, at 1,443,150 to 1,444,514
Atu1448
Atu1449: Atu1449 - potassium uptake protein, at 1,444,563 to 1,445,939
Atu1449
Atu1450: Atu1450 - putative RNA-binding protein Hfq, at 1,446,124 to 1,446,366
Atu1450
Atu1451: Atu1451 - GTP-binding protein HFLX, at 1,446,573 to 1,447,907
Atu1451
Position (kb)
1444
1445
1446 Strain fitness (log2 ratio)
-2
-1
0
1 at 1444.535 kb on - strand at 1444.569 kb on - strand at 1444.587 kb on + strand at 1444.587 kb on + strand at 1444.587 kb on + strand at 1444.603 kb on - strand at 1444.628 kb on + strand at 1444.876 kb on - strand, within Atu1449 at 1444.893 kb on + strand, within Atu1449 at 1445.044 kb on + strand, within Atu1449 at 1445.151 kb on + strand, within Atu1449 at 1445.399 kb on - strand, within Atu1449 at 1445.675 kb on - strand, within Atu1449 at 1445.690 kb on - strand, within Atu1449 at 1445.699 kb on - strand, within Atu1449 at 1445.701 kb on + strand, within Atu1449 at 1445.817 kb on + strand at 1446.382 kb on - strand at 1446.596 kb on - strand at 1446.645 kb on + strand at 1446.764 kb on + strand, within Atu1451 at 1446.767 kb on - strand, within Atu1451 at 1446.817 kb on - strand, within Atu1451 at 1446.855 kb on - strand, within Atu1451 at 1446.884 kb on + strand, within Atu1451 at 1446.891 kb on + strand, within Atu1451 at 1446.891 kb on + strand, within Atu1451 at 1446.915 kb on - strand, within Atu1451
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Azlocillin 0.2 mM remove 1,444,535 - +1.6 1,444,569 - -1.8 1,444,587 + +0.3 1,444,587 + +0.0 1,444,587 + -1.4 1,444,603 - +0.6 1,444,628 + -0.6 1,444,876 - Atu1449 0.23 -0.0 1,444,893 + Atu1449 0.24 +1.2 1,445,044 + Atu1449 0.35 +0.6 1,445,151 + Atu1449 0.43 +0.1 1,445,399 - Atu1449 0.61 +0.6 1,445,675 - Atu1449 0.81 +0.7 1,445,690 - Atu1449 0.82 -0.2 1,445,699 - Atu1449 0.82 -0.2 1,445,701 + Atu1449 0.83 +1.2 1,445,817 + -2.0 1,446,382 - +0.3 1,446,596 - +0.4 1,446,645 + -0.5 1,446,764 + Atu1451 0.14 -0.0 1,446,767 - Atu1451 0.15 +0.9 1,446,817 - Atu1451 0.18 +0.5 1,446,855 - Atu1451 0.21 -0.4 1,446,884 + Atu1451 0.23 +0.5 1,446,891 + Atu1451 0.24 +0.0 1,446,891 + Atu1451 0.24 +0.1 1,446,915 - Atu1451 0.26 +1.0
Or see this region's nucleotide sequence