Experiment: R2A with Azlocillin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0009 and Atu0010 overlap by 4 nucleotides Atu0010 and Atu0011 overlap by 4 nucleotides Atu0011 and Atu0012 are separated by 158 nucleotides
Atu0009: Atu0009 - membrane-bound lytic murein transglycosylase, at 5,865 to 6,977
Atu0009
Atu0010: Atu0010 - hypothetical protein, at 6,974 to 7,543
Atu0010
Atu0011: Atu0011 - transcriptional regulator, at 7,540 to 7,905
Atu0011
Atu0012: Atu0012 - DNA gyrase subunit B, at 8,064 to 10,499
Atu0012
Position (kb) 7
8 Strain fitness (log2 ratio) -1
0
1
2 at 6.660 kb on + strand, within Atu0009 at 6.710 kb on - strand, within Atu0009 at 6.742 kb on + strand, within Atu0009 at 6.801 kb on - strand, within Atu0009 at 6.827 kb on + strand, within Atu0009 at 6.920 kb on - strand at 7.026 kb on - strand at 7.098 kb on + strand, within Atu0010 at 7.148 kb on + strand, within Atu0010 at 7.200 kb on + strand, within Atu0010 at 7.200 kb on - strand, within Atu0010 at 7.208 kb on - strand, within Atu0010 at 7.228 kb on + strand, within Atu0010 at 7.271 kb on + strand, within Atu0010 at 7.334 kb on + strand, within Atu0010 at 7.363 kb on - strand, within Atu0010 at 7.378 kb on - strand, within Atu0010 at 7.378 kb on - strand, within Atu0010 at 7.378 kb on - strand, within Atu0010 at 7.378 kb on - strand, within Atu0010 at 7.405 kb on + strand, within Atu0010 at 7.410 kb on + strand, within Atu0010 at 7.423 kb on - strand, within Atu0010 at 7.761 kb on + strand, within Atu0011 at 7.800 kb on + strand, within Atu0011 at 7.812 kb on + strand, within Atu0011 at 7.866 kb on + strand, within Atu0011 at 7.910 kb on + strand at 7.922 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Azlocillin 0.2 mM remove 6,660 + Atu0009 0.71 +0.3 6,710 - Atu0009 0.76 +0.3 6,742 + Atu0009 0.79 +0.1 6,801 - Atu0009 0.84 -0.1 6,827 + Atu0009 0.86 -0.3 6,920 - -0.7 7,026 - -0.6 7,098 + Atu0010 0.22 -0.3 7,148 + Atu0010 0.31 +0.1 7,200 + Atu0010 0.40 +2.0 7,200 - Atu0010 0.40 +0.4 7,208 - Atu0010 0.41 -0.4 7,228 + Atu0010 0.45 +0.3 7,271 + Atu0010 0.52 -0.4 7,334 + Atu0010 0.63 -0.3 7,363 - Atu0010 0.68 -0.1 7,378 - Atu0010 0.71 -0.1 7,378 - Atu0010 0.71 -0.3 7,378 - Atu0010 0.71 -0.8 7,378 - Atu0010 0.71 +0.2 7,405 + Atu0010 0.76 +1.9 7,410 + Atu0010 0.76 +0.1 7,423 - Atu0010 0.79 -0.8 7,761 + Atu0011 0.60 +0.1 7,800 + Atu0011 0.71 +2.0 7,812 + Atu0011 0.74 +0.3 7,866 + Atu0011 0.89 +0.0 7,910 + -0.5 7,922 + -0.1
Or see this region's nucleotide sequence