Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4846 and Atu4847 are separated by 24 nucleotides Atu4847 and Atu4848 are separated by 19 nucleotides Atu4848 and Atu4849 are separated by 41 nucleotides
Atu4846: Atu4846 - hypothetical protein, at 2,026,305 to 2,027,387
Atu4846
Atu4847: Atu4847 - ribokinase, at 2,027,412 to 2,028,350
Atu4847
Atu4848: Atu4848 - 2-deoxyribose-5-phosphate aldolase, at 2,028,370 to 2,029,329
Atu4848
Atu4849: Atu4849 - NADP-dependent aldehyde dehydrogenase, at 2,029,371 to 2,031,737
Atu4849
Position (kb)
2028
2029
2030 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2027.390 kb on - strand at 2027.390 kb on - strand at 2027.461 kb on + strand at 2027.462 kb on - strand at 2027.542 kb on + strand, within Atu4847 at 2027.544 kb on - strand, within Atu4847 at 2027.577 kb on - strand, within Atu4847 at 2027.621 kb on + strand, within Atu4847 at 2027.625 kb on + strand, within Atu4847 at 2027.692 kb on + strand, within Atu4847 at 2027.736 kb on - strand, within Atu4847 at 2027.780 kb on + strand, within Atu4847 at 2027.786 kb on - strand, within Atu4847 at 2027.796 kb on + strand, within Atu4847 at 2027.807 kb on + strand, within Atu4847 at 2027.850 kb on + strand, within Atu4847 at 2027.884 kb on + strand, within Atu4847 at 2027.970 kb on - strand, within Atu4847 at 2028.012 kb on + strand, within Atu4847 at 2028.020 kb on - strand, within Atu4847 at 2028.031 kb on - strand, within Atu4847 at 2028.076 kb on + strand, within Atu4847 at 2028.142 kb on - strand, within Atu4847 at 2028.157 kb on + strand, within Atu4847 at 2028.163 kb on - strand, within Atu4847 at 2028.205 kb on - strand, within Atu4847 at 2028.219 kb on + strand, within Atu4847 at 2028.219 kb on + strand, within Atu4847 at 2028.219 kb on + strand, within Atu4847 at 2028.219 kb on + strand, within Atu4847 at 2028.225 kb on - strand, within Atu4847 at 2028.227 kb on - strand, within Atu4847 at 2028.241 kb on - strand, within Atu4847 at 2028.245 kb on + strand, within Atu4847 at 2028.262 kb on + strand at 2028.280 kb on - strand at 2028.282 kb on + strand at 2028.282 kb on + strand at 2028.288 kb on + strand at 2028.288 kb on + strand at 2028.296 kb on - strand at 2028.457 kb on - strand at 2028.526 kb on + strand, within Atu4848 at 2028.527 kb on - strand, within Atu4848 at 2028.555 kb on + strand, within Atu4848 at 2028.829 kb on - strand, within Atu4848 at 2028.831 kb on + strand, within Atu4848 at 2028.878 kb on - strand, within Atu4848 at 2028.965 kb on - strand, within Atu4848 at 2028.969 kb on + strand, within Atu4848 at 2029.045 kb on - strand, within Atu4848 at 2029.047 kb on + strand, within Atu4848 at 2029.047 kb on + strand, within Atu4848 at 2029.060 kb on - strand, within Atu4848 at 2029.180 kb on + strand, within Atu4848 at 2029.198 kb on - strand, within Atu4848 at 2029.306 kb on - strand at 2029.377 kb on - strand at 2029.377 kb on - strand at 2029.497 kb on + strand at 2029.608 kb on - strand, within Atu4849 at 2029.681 kb on - strand, within Atu4849 at 2029.830 kb on + strand, within Atu4849 at 2029.935 kb on - strand, within Atu4849 at 2029.994 kb on + strand, within Atu4849 at 2030.004 kb on + strand, within Atu4849 at 2030.142 kb on - strand, within Atu4849 at 2030.168 kb on + strand, within Atu4849 at 2030.221 kb on + strand, within Atu4849 at 2030.239 kb on - strand, within Atu4849 at 2030.251 kb on + strand, within Atu4849
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 2,027,390 - +0.1 2,027,390 - -0.4 2,027,461 + +1.2 2,027,462 - +0.2 2,027,542 + Atu4847 0.14 -0.6 2,027,544 - Atu4847 0.14 -1.0 2,027,577 - Atu4847 0.18 -0.1 2,027,621 + Atu4847 0.22 -0.5 2,027,625 + Atu4847 0.23 +0.1 2,027,692 + Atu4847 0.30 +0.2 2,027,736 - Atu4847 0.35 -0.4 2,027,780 + Atu4847 0.39 +0.4 2,027,786 - Atu4847 0.40 -0.2 2,027,796 + Atu4847 0.41 +0.3 2,027,807 + Atu4847 0.42 +0.3 2,027,850 + Atu4847 0.47 -1.1 2,027,884 + Atu4847 0.50 -0.1 2,027,970 - Atu4847 0.59 -0.2 2,028,012 + Atu4847 0.64 +2.2 2,028,020 - Atu4847 0.65 -1.9 2,028,031 - Atu4847 0.66 -0.4 2,028,076 + Atu4847 0.71 -0.1 2,028,142 - Atu4847 0.78 +1.3 2,028,157 + Atu4847 0.79 +0.6 2,028,163 - Atu4847 0.80 -0.7 2,028,205 - Atu4847 0.84 -1.2 2,028,219 + Atu4847 0.86 -1.0 2,028,219 + Atu4847 0.86 +0.5 2,028,219 + Atu4847 0.86 +0.5 2,028,219 + Atu4847 0.86 +0.8 2,028,225 - Atu4847 0.87 +0.2 2,028,227 - Atu4847 0.87 -0.8 2,028,241 - Atu4847 0.88 +0.6 2,028,245 + Atu4847 0.89 -0.3 2,028,262 + -0.8 2,028,280 - -1.9 2,028,282 + -0.8 2,028,282 + +0.2 2,028,288 + -0.1 2,028,288 + -0.5 2,028,296 - -0.5 2,028,457 - -0.2 2,028,526 + Atu4848 0.16 -0.3 2,028,527 - Atu4848 0.16 -0.7 2,028,555 + Atu4848 0.19 -0.2 2,028,829 - Atu4848 0.48 +0.4 2,028,831 + Atu4848 0.48 +0.4 2,028,878 - Atu4848 0.53 +0.4 2,028,965 - Atu4848 0.62 +0.1 2,028,969 + Atu4848 0.62 -1.1 2,029,045 - Atu4848 0.70 -0.2 2,029,047 + Atu4848 0.71 -2.1 2,029,047 + Atu4848 0.71 -0.2 2,029,060 - Atu4848 0.72 +0.0 2,029,180 + Atu4848 0.84 +0.3 2,029,198 - Atu4848 0.86 -0.1 2,029,306 - -0.5 2,029,377 - +0.7 2,029,377 - -1.9 2,029,497 + -0.2 2,029,608 - Atu4849 0.10 -0.3 2,029,681 - Atu4849 0.13 -0.0 2,029,830 + Atu4849 0.19 +0.2 2,029,935 - Atu4849 0.24 -0.1 2,029,994 + Atu4849 0.26 -0.4 2,030,004 + Atu4849 0.27 -0.6 2,030,142 - Atu4849 0.33 -0.3 2,030,168 + Atu4849 0.34 -0.6 2,030,221 + Atu4849 0.36 -0.7 2,030,239 - Atu4849 0.37 +0.0 2,030,251 + Atu4849 0.37 -0.1
Or see this region's nucleotide sequence