Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4475 and Atu4476 are separated by 238 nucleotides Atu4476 and Atu4477 are separated by 182 nucleotides Atu4477 and Atu4478 are separated by 78 nucleotides
Atu4475: Atu4475 - transporter, at 1,618,245 to 1,620,017
Atu4475
Atu4476: Atu4476 - hypothetical protein, at 1,620,256 to 1,621,017
Atu4476
Atu4477: Atu4477 - TetR family transcriptional regulator, at 1,621,200 to 1,621,886
Atu4477
Atu4478: Atu4478 - multidrug resistance efflux pump, at 1,621,965 to 1,623,263
Atu4478
Position (kb)
1620
1621
1622 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1619.257 kb on - strand, within Atu4475 at 1619.272 kb on - strand, within Atu4475 at 1619.297 kb on + strand, within Atu4475 at 1619.339 kb on - strand, within Atu4475 at 1619.375 kb on + strand, within Atu4475 at 1619.383 kb on - strand, within Atu4475 at 1619.384 kb on - strand, within Atu4475 at 1619.447 kb on - strand, within Atu4475 at 1619.580 kb on + strand, within Atu4475 at 1619.756 kb on - strand, within Atu4475 at 1619.908 kb on - strand at 1619.928 kb on + strand at 1620.005 kb on + strand at 1620.005 kb on + strand at 1620.005 kb on + strand at 1620.013 kb on - strand at 1620.029 kb on - strand at 1620.117 kb on - strand at 1620.134 kb on + strand at 1620.134 kb on + strand at 1620.134 kb on + strand at 1620.142 kb on - strand at 1620.142 kb on - strand at 1620.145 kb on + strand at 1620.153 kb on - strand at 1620.324 kb on + strand at 1620.358 kb on + strand, within Atu4476 at 1620.367 kb on - strand, within Atu4476 at 1620.416 kb on + strand, within Atu4476 at 1620.514 kb on + strand, within Atu4476 at 1620.521 kb on + strand, within Atu4476 at 1620.667 kb on - strand, within Atu4476 at 1620.815 kb on + strand, within Atu4476 at 1620.823 kb on - strand, within Atu4476 at 1620.841 kb on - strand, within Atu4476 at 1620.882 kb on + strand, within Atu4476 at 1620.882 kb on + strand, within Atu4476 at 1620.882 kb on + strand, within Atu4476 at 1620.890 kb on - strand, within Atu4476 at 1620.890 kb on - strand, within Atu4476 at 1620.927 kb on - strand, within Atu4476 at 1620.954 kb on + strand at 1620.969 kb on + strand at 1620.999 kb on - strand at 1621.109 kb on - strand at 1621.198 kb on + strand at 1621.206 kb on - strand at 1621.268 kb on - strand at 1621.347 kb on - strand, within Atu4477 at 1621.358 kb on + strand, within Atu4477 at 1621.476 kb on + strand, within Atu4477 at 1621.512 kb on + strand, within Atu4477 at 1621.517 kb on + strand, within Atu4477 at 1621.520 kb on - strand, within Atu4477 at 1621.557 kb on - strand, within Atu4477 at 1621.559 kb on + strand, within Atu4477 at 1621.559 kb on + strand, within Atu4477 at 1621.567 kb on - strand, within Atu4477 at 1621.575 kb on - strand, within Atu4477 at 1621.684 kb on + strand, within Atu4477 at 1621.691 kb on - strand, within Atu4477 at 1621.745 kb on - strand, within Atu4477 at 1621.745 kb on - strand, within Atu4477 at 1621.745 kb on - strand, within Atu4477 at 1621.807 kb on - strand, within Atu4477 at 1621.872 kb on + strand at 1621.967 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 1,619,257 - Atu4475 0.57 -0.8 1,619,272 - Atu4475 0.58 +0.1 1,619,297 + Atu4475 0.59 -0.2 1,619,339 - Atu4475 0.62 -0.3 1,619,375 + Atu4475 0.64 -0.3 1,619,383 - Atu4475 0.64 -0.3 1,619,384 - Atu4475 0.64 +0.2 1,619,447 - Atu4475 0.68 +0.7 1,619,580 + Atu4475 0.75 +0.2 1,619,756 - Atu4475 0.85 -0.1 1,619,908 - -0.4 1,619,928 + -0.2 1,620,005 + -0.7 1,620,005 + -0.5 1,620,005 + -0.0 1,620,013 - -0.4 1,620,029 - +0.0 1,620,117 - -0.1 1,620,134 + +0.0 1,620,134 + +0.2 1,620,134 + +0.3 1,620,142 - -0.7 1,620,142 - -0.1 1,620,145 + +1.5 1,620,153 - +0.9 1,620,324 + +0.2 1,620,358 + Atu4476 0.13 +1.1 1,620,367 - Atu4476 0.15 -0.2 1,620,416 + Atu4476 0.21 +0.1 1,620,514 + Atu4476 0.34 +0.9 1,620,521 + Atu4476 0.35 -0.0 1,620,667 - Atu4476 0.54 +0.5 1,620,815 + Atu4476 0.73 +0.8 1,620,823 - Atu4476 0.74 +0.1 1,620,841 - Atu4476 0.77 -0.6 1,620,882 + Atu4476 0.82 +0.1 1,620,882 + Atu4476 0.82 +0.1 1,620,882 + Atu4476 0.82 +0.1 1,620,890 - Atu4476 0.83 -0.7 1,620,890 - Atu4476 0.83 +0.7 1,620,927 - Atu4476 0.88 -0.0 1,620,954 + +0.7 1,620,969 + +0.6 1,620,999 - -0.2 1,621,109 - -0.1 1,621,198 + +0.0 1,621,206 - -0.2 1,621,268 - -2.5 1,621,347 - Atu4477 0.21 -1.1 1,621,358 + Atu4477 0.23 -2.7 1,621,476 + Atu4477 0.40 -0.2 1,621,512 + Atu4477 0.45 -1.8 1,621,517 + Atu4477 0.46 -1.8 1,621,520 - Atu4477 0.47 -2.1 1,621,557 - Atu4477 0.52 -0.1 1,621,559 + Atu4477 0.52 -2.1 1,621,559 + Atu4477 0.52 -1.6 1,621,567 - Atu4477 0.53 -1.8 1,621,575 - Atu4477 0.55 -1.9 1,621,684 + Atu4477 0.70 -3.4 1,621,691 - Atu4477 0.71 -0.7 1,621,745 - Atu4477 0.79 -1.9 1,621,745 - Atu4477 0.79 -2.8 1,621,745 - Atu4477 0.79 -1.3 1,621,807 - Atu4477 0.88 -1.9 1,621,872 + -0.4 1,621,967 - +0.2
Or see this region's nucleotide sequence