Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2774 and Atu2775 overlap by 11 nucleotides Atu2775 and Atu2776 are separated by 159 nucleotides Atu2776 and Atu2777 are separated by 158 nucleotides
Atu2774: Atu2774 - carboxy-terminal protease, at 2,778,850 to 2,780,178
Atu2774
Atu2775: Atu2775 - hypothetical protein, at 2,780,168 to 2,781,565
Atu2775
Atu2776: Atu2776 - hypothetical protein, at 2,781,725 to 2,782,207
Atu2776
Atu2777: Atu2777 - hypothetical protein, at 2,782,366 to 2,782,854
Atu2777
Position (kb)
2780
2781
2782 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2780.215 kb on - strand at 2781.622 kb on + strand at 2781.622 kb on + strand at 2781.630 kb on - strand at 2781.630 kb on - strand at 2781.776 kb on - strand, within Atu2776 at 2781.798 kb on - strand, within Atu2776 at 2781.874 kb on + strand, within Atu2776 at 2781.882 kb on - strand, within Atu2776 at 2781.941 kb on + strand, within Atu2776 at 2781.994 kb on - strand, within Atu2776 at 2781.994 kb on - strand, within Atu2776 at 2781.994 kb on - strand, within Atu2776 at 2781.994 kb on - strand, within Atu2776 at 2782.067 kb on + strand, within Atu2776 at 2782.075 kb on - strand, within Atu2776 at 2782.145 kb on - strand, within Atu2776 at 2782.145 kb on - strand, within Atu2776 at 2782.178 kb on - strand at 2782.178 kb on - strand at 2782.222 kb on - strand at 2782.226 kb on + strand at 2782.254 kb on - strand at 2782.349 kb on + strand at 2782.533 kb on - strand, within Atu2777 at 2782.533 kb on - strand, within Atu2777
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 2,780,215 - +3.9 2,781,622 + +0.9 2,781,622 + +1.0 2,781,630 - -0.3 2,781,630 - -0.2 2,781,776 - Atu2776 0.11 +0.1 2,781,798 - Atu2776 0.15 +0.5 2,781,874 + Atu2776 0.31 +0.6 2,781,882 - Atu2776 0.33 +2.3 2,781,941 + Atu2776 0.45 +0.4 2,781,994 - Atu2776 0.56 -0.5 2,781,994 - Atu2776 0.56 -1.0 2,781,994 - Atu2776 0.56 +0.2 2,781,994 - Atu2776 0.56 +0.8 2,782,067 + Atu2776 0.71 +2.2 2,782,075 - Atu2776 0.72 +0.4 2,782,145 - Atu2776 0.87 +1.3 2,782,145 - Atu2776 0.87 +0.5 2,782,178 - +0.5 2,782,178 - +0.3 2,782,222 - -0.1 2,782,226 + +0.3 2,782,254 - -0.0 2,782,349 + +0.6 2,782,533 - Atu2777 0.34 +0.3 2,782,533 - Atu2777 0.34 +1.2
Or see this region's nucleotide sequence