Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2487 and Atu2488 are separated by 177 nucleotides Atu2488 and Atu2489 are separated by 33 nucleotides
Atu2487: Atu2487 - pyridoxamine kinase, at 2,456,221 to 2,457,096
Atu2487
Atu2488: Atu2488 - carbonate dehydratase, at 2,457,274 to 2,457,915
Atu2488
Atu2489: Atu2489 - hypothetical protein, at 2,457,949 to 2,458,764
Atu2489
Position (kb)
2457
2458 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6
7 at 2456.364 kb on - strand, within Atu2487 at 2456.418 kb on + strand, within Atu2487 at 2456.418 kb on + strand, within Atu2487 at 2456.457 kb on + strand, within Atu2487 at 2456.457 kb on + strand, within Atu2487 at 2456.465 kb on - strand, within Atu2487 at 2456.467 kb on + strand, within Atu2487 at 2456.499 kb on + strand, within Atu2487 at 2456.502 kb on - strand, within Atu2487 at 2456.544 kb on - strand, within Atu2487 at 2456.592 kb on + strand, within Atu2487 at 2456.668 kb on - strand, within Atu2487 at 2456.708 kb on - strand, within Atu2487 at 2456.801 kb on + strand, within Atu2487 at 2456.833 kb on + strand, within Atu2487 at 2456.907 kb on + strand, within Atu2487 at 2456.965 kb on + strand, within Atu2487 at 2457.018 kb on + strand at 2457.032 kb on + strand at 2457.037 kb on + strand at 2457.037 kb on + strand at 2457.045 kb on - strand at 2457.180 kb on + strand at 2457.936 kb on + strand at 2457.954 kb on - strand at 2458.107 kb on + strand, within Atu2489 at 2458.197 kb on - strand, within Atu2489 at 2458.218 kb on - strand, within Atu2489 at 2458.225 kb on - strand, within Atu2489 at 2458.294 kb on - strand, within Atu2489 at 2458.456 kb on - strand, within Atu2489 at 2458.466 kb on + strand, within Atu2489 at 2458.494 kb on - strand, within Atu2489 at 2458.494 kb on - strand, within Atu2489 at 2458.494 kb on - strand, within Atu2489 at 2458.535 kb on - strand, within Atu2489 at 2458.581 kb on + strand, within Atu2489 at 2458.605 kb on + strand, within Atu2489 at 2458.718 kb on + strand at 2458.746 kb on - strand at 2458.814 kb on + strand at 2458.822 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 2,456,364 - Atu2487 0.16 -0.2 2,456,418 + Atu2487 0.22 +0.3 2,456,418 + Atu2487 0.22 -0.7 2,456,457 + Atu2487 0.27 +1.1 2,456,457 + Atu2487 0.27 +0.2 2,456,465 - Atu2487 0.28 +1.4 2,456,467 + Atu2487 0.28 +0.7 2,456,499 + Atu2487 0.32 +0.2 2,456,502 - Atu2487 0.32 +0.4 2,456,544 - Atu2487 0.37 +0.6 2,456,592 + Atu2487 0.42 +0.4 2,456,668 - Atu2487 0.51 +1.2 2,456,708 - Atu2487 0.56 +1.0 2,456,801 + Atu2487 0.66 +0.2 2,456,833 + Atu2487 0.70 +0.7 2,456,907 + Atu2487 0.78 +0.4 2,456,965 + Atu2487 0.85 +0.0 2,457,018 + +0.3 2,457,032 + +0.3 2,457,037 + +0.1 2,457,037 + +0.6 2,457,045 - -0.4 2,457,180 + +0.4 2,457,936 + -0.3 2,457,954 - +2.6 2,458,107 + Atu2489 0.19 +6.1 2,458,197 - Atu2489 0.30 +6.8 2,458,218 - Atu2489 0.33 +6.8 2,458,225 - Atu2489 0.34 +7.2 2,458,294 - Atu2489 0.42 +6.6 2,458,456 - Atu2489 0.62 +7.1 2,458,466 + Atu2489 0.63 +6.9 2,458,494 - Atu2489 0.67 +7.1 2,458,494 - Atu2489 0.67 +7.0 2,458,494 - Atu2489 0.67 +6.7 2,458,535 - Atu2489 0.72 +7.1 2,458,581 + Atu2489 0.77 +7.0 2,458,605 + Atu2489 0.80 +7.4 2,458,718 + +5.5 2,458,746 - +6.2 2,458,814 + +1.9 2,458,822 - +3.9
Or see this region's nucleotide sequence