Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2407 and Atu2408 are separated by 106 nucleotides Atu2408 and Atu2409 are separated by 26 nucleotides Atu2409 and Atu2410 are separated by 254 nucleotides
Atu2407: Atu2407 - urease gamma subunit, at 2,376,759 to 2,377,061
Atu2407
Atu2408: Atu2408 - urease accessory protein, at 2,377,168 to 2,377,899
Atu2408
Atu2409: Atu2409 - hypothetical protein, at 2,377,926 to 2,379,182
Atu2409
Atu2410: Atu2410 - ABC transporter, nucleotide binding/ATPase protein (urea/amide), at 2,379,437 to 2,380,132
Atu2410
Position (kb)
2377
2378
2379
2380 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2376.984 kb on + strand, within Atu2407 at 2376.992 kb on - strand, within Atu2407 at 2377.016 kb on - strand, within Atu2407 at 2377.057 kb on - strand at 2377.064 kb on + strand at 2377.083 kb on - strand at 2377.158 kb on + strand at 2377.280 kb on - strand, within Atu2408 at 2377.361 kb on + strand, within Atu2408 at 2377.361 kb on + strand, within Atu2408 at 2377.361 kb on + strand, within Atu2408 at 2377.361 kb on + strand, within Atu2408 at 2377.369 kb on - strand, within Atu2408 at 2377.369 kb on - strand, within Atu2408 at 2377.401 kb on - strand, within Atu2408 at 2377.523 kb on + strand, within Atu2408 at 2377.618 kb on + strand, within Atu2408 at 2377.727 kb on - strand, within Atu2408 at 2377.766 kb on + strand, within Atu2408 at 2377.798 kb on + strand, within Atu2408 at 2377.888 kb on - strand at 2377.917 kb on - strand at 2378.023 kb on - strand at 2378.050 kb on + strand at 2378.096 kb on - strand, within Atu2409 at 2378.144 kb on + strand, within Atu2409 at 2378.162 kb on - strand, within Atu2409 at 2378.308 kb on + strand, within Atu2409 at 2378.308 kb on + strand, within Atu2409 at 2378.414 kb on - strand, within Atu2409 at 2378.449 kb on + strand, within Atu2409 at 2378.479 kb on - strand, within Atu2409 at 2378.592 kb on + strand, within Atu2409 at 2378.683 kb on - strand, within Atu2409 at 2378.727 kb on + strand, within Atu2409 at 2378.735 kb on - strand, within Atu2409 at 2378.783 kb on + strand, within Atu2409 at 2378.843 kb on - strand, within Atu2409 at 2379.069 kb on + strand at 2379.114 kb on + strand at 2379.144 kb on - strand at 2379.179 kb on - strand at 2379.219 kb on + strand at 2379.219 kb on + strand at 2379.277 kb on - strand at 2379.293 kb on + strand at 2379.444 kb on + strand at 2379.473 kb on - strand at 2379.543 kb on + strand, within Atu2410 at 2379.697 kb on - strand, within Atu2410 at 2379.747 kb on + strand, within Atu2410 at 2379.752 kb on + strand, within Atu2410 at 2379.757 kb on + strand, within Atu2410 at 2379.834 kb on - strand, within Atu2410 at 2379.845 kb on - strand, within Atu2410 at 2379.848 kb on + strand, within Atu2410 at 2379.850 kb on + strand, within Atu2410 at 2379.858 kb on - strand, within Atu2410 at 2380.092 kb on - strand at 2380.093 kb on - strand at 2380.098 kb on + strand at 2380.108 kb on - strand at 2380.181 kb on + strand at 2380.181 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 2,376,984 + Atu2407 0.74 +0.2 2,376,992 - Atu2407 0.77 +0.1 2,377,016 - Atu2407 0.85 +0.2 2,377,057 - -0.5 2,377,064 + +1.1 2,377,083 - +0.1 2,377,158 + +0.4 2,377,280 - Atu2408 0.15 +0.1 2,377,361 + Atu2408 0.26 -0.3 2,377,361 + Atu2408 0.26 +0.3 2,377,361 + Atu2408 0.26 +0.6 2,377,361 + Atu2408 0.26 +0.3 2,377,369 - Atu2408 0.27 -0.2 2,377,369 - Atu2408 0.27 -0.1 2,377,401 - Atu2408 0.32 +0.1 2,377,523 + Atu2408 0.48 -1.1 2,377,618 + Atu2408 0.61 -0.6 2,377,727 - Atu2408 0.76 -0.3 2,377,766 + Atu2408 0.82 -0.0 2,377,798 + Atu2408 0.86 -0.5 2,377,888 - -0.2 2,377,917 - -0.3 2,378,023 - +1.1 2,378,050 + -1.5 2,378,096 - Atu2409 0.14 -1.4 2,378,144 + Atu2409 0.17 -2.4 2,378,162 - Atu2409 0.19 -1.9 2,378,308 + Atu2409 0.30 -2.7 2,378,308 + Atu2409 0.30 -2.4 2,378,414 - Atu2409 0.39 -2.8 2,378,449 + Atu2409 0.42 -2.6 2,378,479 - Atu2409 0.44 -2.0 2,378,592 + Atu2409 0.53 -2.0 2,378,683 - Atu2409 0.60 -2.0 2,378,727 + Atu2409 0.64 -1.4 2,378,735 - Atu2409 0.64 -1.5 2,378,783 + Atu2409 0.68 -2.6 2,378,843 - Atu2409 0.73 -1.5 2,379,069 + -0.7 2,379,114 + -2.8 2,379,144 - -2.4 2,379,179 - +0.3 2,379,219 + -1.0 2,379,219 + -1.4 2,379,277 - +0.2 2,379,293 + -1.6 2,379,444 + -1.5 2,379,473 - +0.3 2,379,543 + Atu2410 0.15 +0.1 2,379,697 - Atu2410 0.37 -0.4 2,379,747 + Atu2410 0.45 +0.1 2,379,752 + Atu2410 0.45 +0.3 2,379,757 + Atu2410 0.46 +0.7 2,379,834 - Atu2410 0.57 -1.4 2,379,845 - Atu2410 0.59 +0.3 2,379,848 + Atu2410 0.59 -0.1 2,379,850 + Atu2410 0.59 -0.1 2,379,858 - Atu2410 0.60 -0.2 2,380,092 - -2.3 2,380,093 - -1.0 2,380,098 + -0.4 2,380,108 - -2.2 2,380,181 + -0.6 2,380,181 - -0.3
Or see this region's nucleotide sequence