Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2319 and Atu2320 overlap by 4 nucleotides Atu2320 and Atu2321 are separated by 165 nucleotides Atu2321 and Atu2322 are separated by 1 nucleotides Atu2322 and Atu2323 are separated by 5 nucleotides
Atu2319: Atu2319 - quaternary ammonium compound-resistance protein, at 2,297,366 to 2,297,710
Atu2319
Atu2320: Atu2320 - transcriptional regulator, TetR family, at 2,297,707 to 2,298,291
Atu2320
Atu2321: Atu2321 - D-alanyl-D-alanine carboxypeptidase (penicillin binding protein), at 2,298,457 to 2,299,752
Atu2321
Atu2322: Atu2322 - hypothetical protein, at 2,299,754 to 2,300,497
Atu2322
Atu2323: Atu2323 - hypothetical protein, at 2,300,503 to 2,300,859
Atu2323
Position (kb)
2298
2299
2300 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2297.504 kb on - strand, within Atu2319 at 2297.531 kb on - strand, within Atu2319 at 2297.555 kb on + strand, within Atu2319 at 2297.601 kb on + strand, within Atu2319 at 2297.633 kb on + strand, within Atu2319 at 2297.749 kb on + strand at 2297.872 kb on + strand, within Atu2320 at 2297.933 kb on + strand, within Atu2320 at 2297.941 kb on - strand, within Atu2320 at 2297.941 kb on - strand, within Atu2320 at 2298.004 kb on - strand, within Atu2320 at 2298.242 kb on + strand at 2298.421 kb on - strand at 2298.452 kb on + strand at 2298.455 kb on - strand at 2298.488 kb on + strand at 2298.550 kb on - strand at 2298.555 kb on - strand at 2298.619 kb on + strand, within Atu2321 at 2298.684 kb on - strand, within Atu2321 at 2298.687 kb on - strand, within Atu2321 at 2298.696 kb on - strand, within Atu2321 at 2298.711 kb on - strand, within Atu2321 at 2298.711 kb on - strand, within Atu2321 at 2298.746 kb on + strand, within Atu2321 at 2298.776 kb on + strand, within Atu2321 at 2298.784 kb on - strand, within Atu2321 at 2298.784 kb on - strand, within Atu2321 at 2298.837 kb on + strand, within Atu2321 at 2298.846 kb on - strand, within Atu2321 at 2298.915 kb on + strand, within Atu2321 at 2298.925 kb on + strand, within Atu2321 at 2298.945 kb on - strand, within Atu2321 at 2298.971 kb on - strand, within Atu2321 at 2299.127 kb on - strand, within Atu2321 at 2299.137 kb on - strand, within Atu2321 at 2299.137 kb on - strand, within Atu2321 at 2299.137 kb on - strand, within Atu2321 at 2299.147 kb on + strand, within Atu2321 at 2299.155 kb on - strand, within Atu2321 at 2299.155 kb on - strand, within Atu2321 at 2299.254 kb on + strand, within Atu2321 at 2299.262 kb on - strand, within Atu2321 at 2299.328 kb on + strand, within Atu2321 at 2299.358 kb on - strand, within Atu2321 at 2299.452 kb on + strand, within Atu2321 at 2299.455 kb on - strand, within Atu2321 at 2299.462 kb on + strand, within Atu2321 at 2299.485 kb on + strand, within Atu2321 at 2299.516 kb on - strand, within Atu2321 at 2299.619 kb on - strand, within Atu2321 at 2299.621 kb on + strand, within Atu2321 at 2299.677 kb on + strand at 2299.685 kb on - strand at 2299.719 kb on - strand at 2299.910 kb on + strand, within Atu2322 at 2300.021 kb on + strand, within Atu2322 at 2300.053 kb on + strand, within Atu2322 at 2300.222 kb on + strand, within Atu2322 at 2300.230 kb on - strand, within Atu2322 at 2300.243 kb on - strand, within Atu2322 at 2300.276 kb on + strand, within Atu2322 at 2300.291 kb on + strand, within Atu2322 at 2300.299 kb on + strand, within Atu2322 at 2300.423 kb on - strand at 2300.428 kb on + strand at 2300.500 kb on - strand at 2300.500 kb on - strand at 2300.589 kb on - strand, within Atu2323 at 2300.740 kb on + strand, within Atu2323
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 2,297,504 - Atu2319 0.40 -0.2 2,297,531 - Atu2319 0.48 +0.1 2,297,555 + Atu2319 0.55 +0.1 2,297,601 + Atu2319 0.68 +0.6 2,297,633 + Atu2319 0.77 -0.1 2,297,749 + +0.6 2,297,872 + Atu2320 0.28 -0.8 2,297,933 + Atu2320 0.39 -0.8 2,297,941 - Atu2320 0.40 +0.1 2,297,941 - Atu2320 0.40 +0.2 2,298,004 - Atu2320 0.51 +0.6 2,298,242 + -0.3 2,298,421 - -0.1 2,298,452 + -0.7 2,298,455 - -0.1 2,298,488 + -0.5 2,298,550 - +0.2 2,298,555 - -0.3 2,298,619 + Atu2321 0.12 +0.1 2,298,684 - Atu2321 0.18 -0.4 2,298,687 - Atu2321 0.18 +0.4 2,298,696 - Atu2321 0.18 +0.4 2,298,711 - Atu2321 0.20 +0.3 2,298,711 - Atu2321 0.20 -1.2 2,298,746 + Atu2321 0.22 +0.0 2,298,776 + Atu2321 0.25 -0.9 2,298,784 - Atu2321 0.25 -0.2 2,298,784 - Atu2321 0.25 -0.5 2,298,837 + Atu2321 0.29 -0.4 2,298,846 - Atu2321 0.30 +0.1 2,298,915 + Atu2321 0.35 +0.6 2,298,925 + Atu2321 0.36 +0.2 2,298,945 - Atu2321 0.38 -0.2 2,298,971 - Atu2321 0.40 +0.7 2,299,127 - Atu2321 0.52 +0.1 2,299,137 - Atu2321 0.52 -0.1 2,299,137 - Atu2321 0.52 +0.1 2,299,137 - Atu2321 0.52 +0.0 2,299,147 + Atu2321 0.53 +0.3 2,299,155 - Atu2321 0.54 +1.4 2,299,155 - Atu2321 0.54 +0.2 2,299,254 + Atu2321 0.61 +0.8 2,299,262 - Atu2321 0.62 +0.4 2,299,328 + Atu2321 0.67 +0.1 2,299,358 - Atu2321 0.70 +0.2 2,299,452 + Atu2321 0.77 +2.3 2,299,455 - Atu2321 0.77 -0.4 2,299,462 + Atu2321 0.78 +0.1 2,299,485 + Atu2321 0.79 -0.3 2,299,516 - Atu2321 0.82 +0.3 2,299,619 - Atu2321 0.90 -0.2 2,299,621 + Atu2321 0.90 -0.3 2,299,677 + +0.7 2,299,685 - +0.3 2,299,719 - +0.4 2,299,910 + Atu2322 0.21 -0.5 2,300,021 + Atu2322 0.36 -0.0 2,300,053 + Atu2322 0.40 +0.9 2,300,222 + Atu2322 0.63 -0.2 2,300,230 - Atu2322 0.64 +0.3 2,300,243 - Atu2322 0.66 -0.0 2,300,276 + Atu2322 0.70 +1.5 2,300,291 + Atu2322 0.72 -0.9 2,300,299 + Atu2322 0.73 +0.3 2,300,423 - -0.6 2,300,428 + -0.5 2,300,500 - -1.4 2,300,500 - -0.2 2,300,589 - Atu2323 0.24 -0.1 2,300,740 + Atu2323 0.66 -1.8
Or see this region's nucleotide sequence