Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2227 and Atu2228 are separated by 302 nucleotides Atu2228 and Atu2229 are separated by 17 nucleotides Atu2229 and Atu2230 are separated by 100 nucleotides
Atu2227: Atu2227 - 50S ribosomal protein L25, at 2,198,732 to 2,199,340
Atu2227
Atu2228: Atu2228 - GGDEF family protein, at 2,199,643 to 2,202,030
Atu2228
Atu2229: Atu2229 - ABC transporter, substrate binding protein (amino acid), at 2,202,048 to 2,202,761
Atu2229
Atu2230: Atu2230 - peptidyl tRNA hydrolase, at 2,202,862 to 2,203,578
Atu2230
Position (kb)
2199
2200
2201
2202
2203 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6
7 at 2199.516 kb on - strand at 2199.516 kb on - strand at 2199.633 kb on - strand at 2199.639 kb on - strand at 2199.700 kb on - strand at 2199.802 kb on + strand at 2199.810 kb on - strand at 2199.815 kb on - strand at 2199.900 kb on + strand, within Atu2228 at 2199.913 kb on - strand, within Atu2228 at 2199.915 kb on + strand, within Atu2228 at 2200.018 kb on - strand, within Atu2228 at 2200.018 kb on - strand, within Atu2228 at 2200.028 kb on - strand, within Atu2228 at 2200.039 kb on + strand, within Atu2228 at 2200.113 kb on - strand, within Atu2228 at 2200.164 kb on + strand, within Atu2228 at 2200.205 kb on + strand, within Atu2228 at 2200.216 kb on + strand, within Atu2228 at 2200.343 kb on + strand, within Atu2228 at 2200.412 kb on - strand, within Atu2228 at 2200.414 kb on - strand, within Atu2228 at 2200.414 kb on - strand, within Atu2228 at 2200.490 kb on + strand, within Atu2228 at 2200.523 kb on - strand, within Atu2228 at 2200.523 kb on - strand, within Atu2228 at 2200.523 kb on - strand, within Atu2228 at 2200.536 kb on + strand, within Atu2228 at 2200.714 kb on + strand, within Atu2228 at 2200.754 kb on + strand, within Atu2228 at 2200.780 kb on + strand, within Atu2228 at 2200.897 kb on + strand, within Atu2228 at 2200.923 kb on - strand, within Atu2228 at 2200.984 kb on - strand, within Atu2228 at 2201.018 kb on - strand, within Atu2228 at 2201.049 kb on - strand, within Atu2228 at 2201.085 kb on + strand, within Atu2228 at 2201.140 kb on + strand, within Atu2228 at 2201.205 kb on - strand, within Atu2228 at 2201.205 kb on - strand, within Atu2228 at 2201.280 kb on - strand, within Atu2228 at 2201.295 kb on - strand, within Atu2228 at 2201.303 kb on - strand, within Atu2228 at 2201.336 kb on + strand, within Atu2228 at 2201.364 kb on - strand, within Atu2228 at 2201.482 kb on + strand, within Atu2228 at 2201.482 kb on + strand, within Atu2228 at 2201.490 kb on - strand, within Atu2228 at 2201.584 kb on + strand, within Atu2228 at 2201.685 kb on + strand, within Atu2228 at 2201.761 kb on + strand, within Atu2228 at 2201.810 kb on + strand at 2201.810 kb on + strand at 2201.810 kb on + strand at 2201.942 kb on - strand at 2201.952 kb on + strand at 2202.155 kb on - strand, within Atu2229 at 2202.181 kb on + strand, within Atu2229 at 2202.191 kb on + strand, within Atu2229 at 2202.191 kb on + strand, within Atu2229 at 2202.199 kb on - strand, within Atu2229 at 2202.199 kb on - strand, within Atu2229 at 2202.199 kb on - strand, within Atu2229 at 2202.199 kb on - strand, within Atu2229 at 2202.256 kb on + strand, within Atu2229 at 2202.264 kb on - strand, within Atu2229 at 2202.266 kb on + strand, within Atu2229 at 2202.416 kb on + strand, within Atu2229 at 2202.431 kb on + strand, within Atu2229 at 2202.439 kb on - strand, within Atu2229 at 2202.606 kb on - strand, within Atu2229 at 2202.663 kb on + strand, within Atu2229 at 2202.794 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 2,199,516 - -0.9 2,199,516 - -1.5 2,199,633 - -0.1 2,199,639 - -0.2 2,199,700 - +2.7 2,199,802 + +0.2 2,199,810 - -0.3 2,199,815 - +0.6 2,199,900 + Atu2228 0.11 +0.9 2,199,913 - Atu2228 0.11 -0.1 2,199,915 + Atu2228 0.11 +0.1 2,200,018 - Atu2228 0.16 +0.2 2,200,018 - Atu2228 0.16 +0.7 2,200,028 - Atu2228 0.16 -0.2 2,200,039 + Atu2228 0.17 +0.6 2,200,113 - Atu2228 0.20 -1.0 2,200,164 + Atu2228 0.22 +0.0 2,200,205 + Atu2228 0.24 -0.6 2,200,216 + Atu2228 0.24 +0.0 2,200,343 + Atu2228 0.29 +0.7 2,200,412 - Atu2228 0.32 +6.8 2,200,414 - Atu2228 0.32 -0.5 2,200,414 - Atu2228 0.32 -0.3 2,200,490 + Atu2228 0.35 +0.0 2,200,523 - Atu2228 0.37 -0.3 2,200,523 - Atu2228 0.37 -0.0 2,200,523 - Atu2228 0.37 -0.0 2,200,536 + Atu2228 0.37 +0.1 2,200,714 + Atu2228 0.45 +1.3 2,200,754 + Atu2228 0.47 +0.2 2,200,780 + Atu2228 0.48 +0.9 2,200,897 + Atu2228 0.53 +0.6 2,200,923 - Atu2228 0.54 +0.5 2,200,984 - Atu2228 0.56 -1.4 2,201,018 - Atu2228 0.58 +0.3 2,201,049 - Atu2228 0.59 +0.2 2,201,085 + Atu2228 0.60 -0.2 2,201,140 + Atu2228 0.63 -0.6 2,201,205 - Atu2228 0.65 +0.2 2,201,205 - Atu2228 0.65 -0.0 2,201,280 - Atu2228 0.69 +0.5 2,201,295 - Atu2228 0.69 +0.4 2,201,303 - Atu2228 0.70 -1.0 2,201,336 + Atu2228 0.71 +0.6 2,201,364 - Atu2228 0.72 -1.4 2,201,482 + Atu2228 0.77 +2.1 2,201,482 + Atu2228 0.77 +0.2 2,201,490 - Atu2228 0.77 -0.3 2,201,584 + Atu2228 0.81 -0.7 2,201,685 + Atu2228 0.86 -0.2 2,201,761 + Atu2228 0.89 +0.5 2,201,810 + +0.1 2,201,810 + -0.0 2,201,810 + -1.1 2,201,942 - -0.7 2,201,952 + -0.1 2,202,155 - Atu2229 0.15 -0.4 2,202,181 + Atu2229 0.19 +0.1 2,202,191 + Atu2229 0.20 -0.6 2,202,191 + Atu2229 0.20 -0.5 2,202,199 - Atu2229 0.21 +0.1 2,202,199 - Atu2229 0.21 -1.6 2,202,199 - Atu2229 0.21 +1.0 2,202,199 - Atu2229 0.21 +0.2 2,202,256 + Atu2229 0.29 +0.2 2,202,264 - Atu2229 0.30 -1.0 2,202,266 + Atu2229 0.31 +0.3 2,202,416 + Atu2229 0.52 -1.0 2,202,431 + Atu2229 0.54 -0.2 2,202,439 - Atu2229 0.55 +0.1 2,202,606 - Atu2229 0.78 -0.0 2,202,663 + Atu2229 0.86 +1.2 2,202,794 - -0.3
Or see this region's nucleotide sequence