Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1783 and Atu1784 are separated by 119 nucleotides Atu1784 and Atu1785 are separated by 13 nucleotides Atu1785 and Atu1786 overlap by 4 nucleotides
Atu1783: Atu1783 - hypothetical protein, at 1,769,199 to 1,769,444
Atu1783
Atu1784: Atu1784 - hypothetical protein, at 1,769,564 to 1,770,232
Atu1784
Atu1785: Atu1785 - acetyltransferase, at 1,770,246 to 1,770,749
Atu1785
Atu1786: Atu1786 - glucosamine-fructose-6-phosphate aminotransferase, at 1,770,746 to 1,772,572
Atu1786
Position (kb)
1770
1771 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1769.541 kb on + strand at 1769.744 kb on - strand, within Atu1784 at 1769.888 kb on - strand, within Atu1784 at 1769.985 kb on - strand, within Atu1784 at 1770.030 kb on + strand, within Atu1784 at 1770.081 kb on + strand, within Atu1784 at 1770.091 kb on + strand, within Atu1784 at 1770.131 kb on - strand, within Atu1784 at 1770.146 kb on + strand, within Atu1784 at 1770.169 kb on - strand at 1770.251 kb on - strand at 1770.251 kb on - strand at 1770.359 kb on - strand, within Atu1785 at 1770.407 kb on + strand, within Atu1785 at 1770.486 kb on - strand, within Atu1785 at 1770.497 kb on + strand, within Atu1785 at 1770.505 kb on - strand, within Atu1785 at 1770.517 kb on + strand, within Atu1785 at 1770.520 kb on - strand, within Atu1785 at 1770.546 kb on - strand, within Atu1785 at 1770.620 kb on - strand, within Atu1785 at 1770.656 kb on - strand, within Atu1785 at 1770.669 kb on + strand, within Atu1785 at 1770.716 kb on + strand at 1770.744 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 1,769,541 + -0.6 1,769,744 - Atu1784 0.27 -1.0 1,769,888 - Atu1784 0.48 -1.5 1,769,985 - Atu1784 0.63 -0.9 1,770,030 + Atu1784 0.70 +0.1 1,770,081 + Atu1784 0.77 -1.4 1,770,091 + Atu1784 0.79 -1.5 1,770,131 - Atu1784 0.85 -0.8 1,770,146 + Atu1784 0.87 -1.9 1,770,169 - -1.0 1,770,251 - +0.9 1,770,251 - +0.6 1,770,359 - Atu1785 0.22 +0.4 1,770,407 + Atu1785 0.32 +1.0 1,770,486 - Atu1785 0.48 -0.8 1,770,497 + Atu1785 0.50 +0.2 1,770,505 - Atu1785 0.51 -0.3 1,770,517 + Atu1785 0.54 +0.2 1,770,520 - Atu1785 0.54 -0.3 1,770,546 - Atu1785 0.60 -1.2 1,770,620 - Atu1785 0.74 -0.3 1,770,656 - Atu1785 0.81 -0.4 1,770,669 + Atu1785 0.84 +0.1 1,770,716 + -2.9 1,770,744 - -0.5
Or see this region's nucleotide sequence