Strain Fitness in Agrobacterium fabrum C58 around Atu1714

Experiment: R2A with Vancomycin 0.009 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAtu1712 and Atu1713 are separated by 214 nucleotidesAtu1713 and Atu1714 are separated by 301 nucleotidesAtu1714 and Atu1715 are separated by 42 nucleotidesAtu1715 and Atu1716 overlap by 14 nucleotides Atu1712: Atu1712 - deoxyguanosinetriphosphate triphosphohydrolase, at 1,699,492 to 1,700,709 Atu1712 Atu1713: Atu1713 - hypothetical protein, at 1,700,924 to 1,701,256 Atu1713 Atu1714: Atu1714 - exonuclease III, at 1,701,558 to 1,702,355 Atu1714 Atu1715: Atu1715 - exopolysaccharide production negative regulator, at 1,702,398 to 1,703,192 Atu1715 Atu1716: Atu1716 - hypothetical protein, at 1,703,179 to 1,703,496 Atu1716 Position (kb) 1701 1702 1703Strain fitness (log2 ratio) -1 0 1 2 3 4at 1700.586 kb on + strand, within Atu1712at 1700.590 kb on - strandat 1700.697 kb on + strandat 1700.713 kb on - strandat 1700.778 kb on + strandat 1700.789 kb on + strandat 1700.796 kb on + strandat 1700.804 kb on - strandat 1700.804 kb on - strandat 1700.828 kb on + strandat 1700.828 kb on + strandat 1700.836 kb on - strandat 1700.847 kb on - strandat 1701.363 kb on + strandat 1701.408 kb on + strandat 1701.432 kb on + strandat 1701.443 kb on + strandat 1701.478 kb on + strandat 1701.533 kb on - strandat 1702.147 kb on - strand, within Atu1714at 1702.382 kb on + strandat 1703.212 kb on - strand, within Atu1716

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM
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1,700,586 + Atu1712 0.90 -1.2
1,700,590 - +0.3
1,700,697 + -0.7
1,700,713 - -0.3
1,700,778 + -0.2
1,700,789 + +0.1
1,700,796 + -0.4
1,700,804 - +0.1
1,700,804 - -0.6
1,700,828 + -0.2
1,700,828 + -0.0
1,700,836 - -0.1
1,700,847 - -1.2
1,701,363 + -0.1
1,701,408 + -0.6
1,701,432 + -0.1
1,701,443 + -0.2
1,701,478 + +0.3
1,701,533 - +0.6
1,702,147 - Atu1714 0.74 +4.4
1,702,382 + -0.1
1,703,212 - Atu1716 0.10 +4.0

Or see this region's nucleotide sequence