Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1712 and Atu1713 are separated by 214 nucleotides Atu1713 and Atu1714 are separated by 301 nucleotides Atu1714 and Atu1715 are separated by 42 nucleotides Atu1715 and Atu1716 overlap by 14 nucleotides
Atu1712: Atu1712 - deoxyguanosinetriphosphate triphosphohydrolase, at 1,699,492 to 1,700,709
Atu1712
Atu1713: Atu1713 - hypothetical protein, at 1,700,924 to 1,701,256
Atu1713
Atu1714: Atu1714 - exonuclease III, at 1,701,558 to 1,702,355
Atu1714
Atu1715: Atu1715 - exopolysaccharide production negative regulator, at 1,702,398 to 1,703,192
Atu1715
Atu1716: Atu1716 - hypothetical protein, at 1,703,179 to 1,703,496
Atu1716
Position (kb)
1701
1702
1703 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1700.586 kb on + strand, within Atu1712 at 1700.590 kb on - strand at 1700.697 kb on + strand at 1700.713 kb on - strand at 1700.778 kb on + strand at 1700.789 kb on + strand at 1700.796 kb on + strand at 1700.804 kb on - strand at 1700.804 kb on - strand at 1700.828 kb on + strand at 1700.828 kb on + strand at 1700.836 kb on - strand at 1700.847 kb on - strand at 1701.363 kb on + strand at 1701.408 kb on + strand at 1701.432 kb on + strand at 1701.443 kb on + strand at 1701.478 kb on + strand at 1701.533 kb on - strand at 1702.147 kb on - strand, within Atu1714 at 1702.382 kb on + strand at 1703.212 kb on - strand, within Atu1716
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 1,700,586 + Atu1712 0.90 -1.2 1,700,590 - +0.3 1,700,697 + -0.7 1,700,713 - -0.3 1,700,778 + -0.2 1,700,789 + +0.1 1,700,796 + -0.4 1,700,804 - +0.1 1,700,804 - -0.6 1,700,828 + -0.2 1,700,828 + -0.0 1,700,836 - -0.1 1,700,847 - -1.2 1,701,363 + -0.1 1,701,408 + -0.6 1,701,432 + -0.1 1,701,443 + -0.2 1,701,478 + +0.3 1,701,533 - +0.6 1,702,147 - Atu1714 0.74 +4.4 1,702,382 + -0.1 1,703,212 - Atu1716 0.10 +4.0
Or see this region's nucleotide sequence