Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1701 and Atu1702 are separated by 8 nucleotides Atu1702 and Atu1703 are separated by 212 nucleotides Atu1703 and Atu1704 are separated by 99 nucleotides Atu1704 and Atu1705 overlap by 4 nucleotides
Atu1701: Atu1701 - protein-L-isoaspartate O-methyltransferase, at 1,686,722 to 1,687,363
Atu1701
Atu1702: Atu1702 - stationary-phase survival protein, at 1,687,372 to 1,688,142
Atu1702
Atu1703: Atu1703 - seryl-tRNA synthetase, at 1,688,355 to 1,689,638
Atu1703
Atu1704: Atu1704 - SEC-independent protein translocase protein, at 1,689,738 to 1,690,541
Atu1704
Atu1705: Atu1705 - SEC-independent protein translocase protein, at 1,690,538 to 1,691,278
Atu1705
Position (kb)
1688
1689
1690 Strain fitness (log2 ratio)
-2
-1
0
1 at 1687.414 kb on - strand at 1687.434 kb on + strand at 1687.439 kb on + strand at 1687.470 kb on + strand, within Atu1702 at 1687.505 kb on + strand, within Atu1702 at 1687.532 kb on - strand, within Atu1702 at 1687.602 kb on - strand, within Atu1702 at 1687.620 kb on + strand, within Atu1702 at 1687.858 kb on - strand, within Atu1702 at 1687.951 kb on - strand, within Atu1702 at 1687.981 kb on - strand, within Atu1702 at 1688.009 kb on - strand, within Atu1702 at 1688.048 kb on + strand, within Atu1702 at 1688.055 kb on - strand, within Atu1702 at 1688.091 kb on + strand at 1688.186 kb on - strand at 1688.210 kb on - strand at 1688.243 kb on + strand at 1688.321 kb on + strand at 1688.327 kb on + strand at 1688.335 kb on - strand at 1688.352 kb on + strand at 1688.352 kb on + strand at 1688.360 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 1,687,414 - +0.5 1,687,434 + -1.3 1,687,439 + -0.3 1,687,470 + Atu1702 0.13 +0.5 1,687,505 + Atu1702 0.17 -0.7 1,687,532 - Atu1702 0.21 +0.8 1,687,602 - Atu1702 0.30 +0.3 1,687,620 + Atu1702 0.32 +0.3 1,687,858 - Atu1702 0.63 +0.4 1,687,951 - Atu1702 0.75 +0.3 1,687,981 - Atu1702 0.79 -2.3 1,688,009 - Atu1702 0.83 +0.6 1,688,048 + Atu1702 0.88 +0.9 1,688,055 - Atu1702 0.89 -1.0 1,688,091 + +0.7 1,688,186 - -1.0 1,688,210 - +0.6 1,688,243 + +0.6 1,688,321 + +0.1 1,688,327 + +0.6 1,688,335 - +0.8 1,688,352 + +0.2 1,688,352 + +0.4 1,688,360 - -0.0
Or see this region's nucleotide sequence