Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1377 and Atu1378 are separated by 64 nucleotides Atu1378 and Atu1379 overlap by 1 nucleotides Atu1379 and Atu1380 are separated by 37 nucleotides
Atu1377: Atu1377 - ribosome recycling factor, at 1,370,945 to 1,371,502
Atu1377
Atu1378: Atu1378 - undecaprenyl pyrophosphate synthase, at 1,371,567 to 1,372,310
Atu1378
Atu1379: Atu1379 - phosphatidate cytidylyltransferase, at 1,372,310 to 1,373,143
Atu1379
Atu1380: Atu1380 - zinc metallopeptidase, at 1,373,181 to 1,374,314
Atu1380
Position (kb)
1372
1373
1374 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6
7
8 at 1373.097 kb on + strand at 1373.186 kb on + strand at 1373.218 kb on + strand at 1373.226 kb on - strand at 1373.329 kb on - strand, within Atu1380 at 1373.335 kb on + strand, within Atu1380 at 1373.460 kb on - strand, within Atu1380 at 1373.804 kb on + strand, within Atu1380 at 1373.819 kb on - strand, within Atu1380 at 1373.830 kb on + strand, within Atu1380 at 1373.936 kb on + strand, within Atu1380 at 1373.936 kb on + strand, within Atu1380 at 1373.944 kb on - strand, within Atu1380 at 1374.004 kb on - strand, within Atu1380 at 1374.131 kb on + strand, within Atu1380 at 1374.131 kb on + strand, within Atu1380
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 1,373,097 + +3.1 1,373,186 + +7.2 1,373,218 + +6.9 1,373,226 - +7.3 1,373,329 - Atu1380 0.13 +7.7 1,373,335 + Atu1380 0.14 +7.3 1,373,460 - Atu1380 0.25 +7.2 1,373,804 + Atu1380 0.55 +6.9 1,373,819 - Atu1380 0.56 +7.1 1,373,830 + Atu1380 0.57 +7.6 1,373,936 + Atu1380 0.67 +8.0 1,373,936 + Atu1380 0.67 +7.5 1,373,944 - Atu1380 0.67 +7.0 1,374,004 - Atu1380 0.73 +7.5 1,374,131 + Atu1380 0.84 +7.7 1,374,131 + Atu1380 0.84 +8.0
Or see this region's nucleotide sequence