Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1373 and Atu1374 are separated by 193 nucleotides Atu1374 and Atu1375 are separated by 359 nucleotides
Atu1373: Atu1373 - hypothetical protein, at 1,366,969 to 1,367,811
Atu1373
Atu1374: Atu1374 - 30S ribosomal protein S2, at 1,368,005 to 1,368,772
Atu1374
Atu1375: Atu1375 - translation elongation factor Ts, at 1,369,132 to 1,370,058
Atu1375
Position (kb)
1368
1369 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1367.007 kb on + strand at 1367.007 kb on + strand at 1367.022 kb on + strand at 1367.030 kb on - strand at 1367.120 kb on + strand, within Atu1373 at 1367.208 kb on + strand, within Atu1373 at 1367.247 kb on + strand, within Atu1373 at 1367.247 kb on + strand, within Atu1373 at 1367.247 kb on + strand, within Atu1373 at 1367.278 kb on + strand, within Atu1373 at 1367.291 kb on + strand, within Atu1373 at 1367.298 kb on + strand, within Atu1373 at 1367.298 kb on + strand, within Atu1373 at 1367.298 kb on + strand, within Atu1373 at 1367.306 kb on - strand, within Atu1373 at 1367.306 kb on - strand, within Atu1373 at 1367.327 kb on - strand, within Atu1373 at 1367.327 kb on - strand, within Atu1373 at 1367.327 kb on - strand, within Atu1373 at 1367.346 kb on - strand, within Atu1373 at 1367.402 kb on + strand, within Atu1373 at 1367.498 kb on + strand, within Atu1373 at 1367.506 kb on - strand, within Atu1373 at 1367.506 kb on - strand, within Atu1373 at 1367.528 kb on + strand, within Atu1373 at 1367.536 kb on - strand, within Atu1373 at 1367.586 kb on + strand, within Atu1373 at 1367.732 kb on - strand at 1367.786 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 1,367,007 + +1.9 1,367,007 + +0.3 1,367,022 + +0.5 1,367,030 - +1.2 1,367,120 + Atu1373 0.18 +0.5 1,367,208 + Atu1373 0.28 +2.2 1,367,247 + Atu1373 0.33 -0.3 1,367,247 + Atu1373 0.33 -0.0 1,367,247 + Atu1373 0.33 +3.6 1,367,278 + Atu1373 0.37 -0.2 1,367,291 + Atu1373 0.38 -0.3 1,367,298 + Atu1373 0.39 +1.4 1,367,298 + Atu1373 0.39 +0.6 1,367,298 + Atu1373 0.39 +0.3 1,367,306 - Atu1373 0.40 +2.1 1,367,306 - Atu1373 0.40 +0.5 1,367,327 - Atu1373 0.42 +2.2 1,367,327 - Atu1373 0.42 +0.8 1,367,327 - Atu1373 0.42 +1.1 1,367,346 - Atu1373 0.45 +2.1 1,367,402 + Atu1373 0.51 +2.4 1,367,498 + Atu1373 0.63 +2.1 1,367,506 - Atu1373 0.64 +2.0 1,367,506 - Atu1373 0.64 +0.5 1,367,528 + Atu1373 0.66 +0.7 1,367,536 - Atu1373 0.67 +0.7 1,367,586 + Atu1373 0.73 +0.2 1,367,732 - +0.0 1,367,786 - -1.3
Or see this region's nucleotide sequence