Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0830 and Atu8138 are separated by 299 nucleotides Atu8138 and Atu0832 are separated by 173 nucleotides Atu0832 and Atu8139 are separated by 4 nucleotides Atu8139 and Atu0834 are separated by 28 nucleotides
Atu0830: Atu0830 - choline dehydrogenase, at 828,964 to 830,613
Atu0830
Atu8138: Atu8138 - hypothetical protein, at 830,913 to 831,188
Atu8138
Atu0832: Atu0832 - chloride channel protein, at 831,362 to 832,678
Atu0832
Atu8139: Atu8139 - hypothetical protein, at 832,683 to 833,480
Atu8139
Atu0834: Atu0834 - hypothetical protein, at 833,509 to 834,507
Atu0834
Position (kb) 831
832
833 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 830.569 kb on - strand at 830.587 kb on - strand at 830.620 kb on + strand at 830.810 kb on + strand at 830.811 kb on + strand at 830.834 kb on + strand at 830.853 kb on + strand at 830.853 kb on + strand at 830.866 kb on - strand at 830.947 kb on + strand, within Atu8138 at 831.025 kb on + strand, within Atu8138 at 831.259 kb on - strand at 831.372 kb on - strand at 831.383 kb on + strand at 831.408 kb on - strand at 831.461 kb on + strand at 831.561 kb on + strand, within Atu0832 at 831.569 kb on - strand, within Atu0832 at 831.730 kb on - strand, within Atu0832 at 831.764 kb on + strand, within Atu0832 at 831.764 kb on + strand, within Atu0832 at 831.820 kb on + strand, within Atu0832 at 831.820 kb on + strand, within Atu0832 at 831.820 kb on + strand, within Atu0832 at 831.820 kb on + strand, within Atu0832 at 831.820 kb on + strand, within Atu0832 at 831.820 kb on + strand, within Atu0832 at 831.828 kb on - strand, within Atu0832 at 831.828 kb on - strand, within Atu0832 at 831.828 kb on - strand, within Atu0832 at 831.828 kb on - strand, within Atu0832 at 831.828 kb on - strand, within Atu0832 at 831.913 kb on + strand, within Atu0832 at 831.942 kb on + strand, within Atu0832 at 831.950 kb on - strand, within Atu0832 at 831.961 kb on + strand, within Atu0832 at 832.063 kb on + strand, within Atu0832 at 832.094 kb on + strand, within Atu0832 at 832.125 kb on - strand, within Atu0832 at 832.176 kb on - strand, within Atu0832 at 832.292 kb on + strand, within Atu0832 at 832.414 kb on + strand, within Atu0832 at 832.433 kb on - strand, within Atu0832 at 832.532 kb on - strand, within Atu0832 at 832.626 kb on + strand at 832.658 kb on + strand at 832.658 kb on + strand at 832.673 kb on + strand at 832.837 kb on - strand, within Atu8139 at 832.949 kb on - strand, within Atu8139 at 832.949 kb on - strand, within Atu8139 at 832.971 kb on - strand, within Atu8139 at 832.989 kb on - strand, within Atu8139 at 833.011 kb on - strand, within Atu8139 at 833.077 kb on + strand, within Atu8139 at 833.137 kb on - strand, within Atu8139 at 833.171 kb on + strand, within Atu8139 at 833.206 kb on + strand, within Atu8139 at 833.206 kb on + strand, within Atu8139 at 833.206 kb on + strand, within Atu8139 at 833.214 kb on - strand, within Atu8139 at 833.214 kb on - strand, within Atu8139 at 833.309 kb on - strand, within Atu8139 at 833.626 kb on - strand, within Atu0834
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 830,569 - -0.8 830,587 - +0.1 830,620 + +1.6 830,810 + +1.5 830,811 + +0.2 830,834 + -0.6 830,853 + -0.8 830,853 + +0.9 830,866 - -0.5 830,947 + Atu8138 0.12 -1.3 831,025 + Atu8138 0.41 -1.5 831,259 - -0.1 831,372 - -0.2 831,383 + -0.3 831,408 - -1.1 831,461 + -3.3 831,561 + Atu0832 0.15 -1.3 831,569 - Atu0832 0.16 -0.2 831,730 - Atu0832 0.28 -0.8 831,764 + Atu0832 0.31 -1.6 831,764 + Atu0832 0.31 +1.3 831,820 + Atu0832 0.35 +0.0 831,820 + Atu0832 0.35 -1.3 831,820 + Atu0832 0.35 -0.1 831,820 + Atu0832 0.35 -1.2 831,820 + Atu0832 0.35 +0.3 831,820 + Atu0832 0.35 -0.0 831,828 - Atu0832 0.35 -0.5 831,828 - Atu0832 0.35 -0.3 831,828 - Atu0832 0.35 +0.8 831,828 - Atu0832 0.35 -1.1 831,828 - Atu0832 0.35 -0.4 831,913 + Atu0832 0.42 +0.2 831,942 + Atu0832 0.44 -1.3 831,950 - Atu0832 0.45 -0.0 831,961 + Atu0832 0.45 +0.2 832,063 + Atu0832 0.53 +0.3 832,094 + Atu0832 0.56 +0.7 832,125 - Atu0832 0.58 +1.1 832,176 - Atu0832 0.62 -0.5 832,292 + Atu0832 0.71 -0.6 832,414 + Atu0832 0.80 +0.1 832,433 - Atu0832 0.81 -1.4 832,532 - Atu0832 0.89 -0.3 832,626 + +1.1 832,658 + +0.4 832,658 + -0.6 832,673 + +0.6 832,837 - Atu8139 0.19 -0.9 832,949 - Atu8139 0.33 -0.2 832,949 - Atu8139 0.33 +0.1 832,971 - Atu8139 0.36 -0.4 832,989 - Atu8139 0.38 -0.3 833,011 - Atu8139 0.41 -0.2 833,077 + Atu8139 0.49 -0.1 833,137 - Atu8139 0.57 +0.2 833,171 + Atu8139 0.61 -0.6 833,206 + Atu8139 0.66 -0.6 833,206 + Atu8139 0.66 -1.6 833,206 + Atu8139 0.66 +0.5 833,214 - Atu8139 0.67 +0.0 833,214 - Atu8139 0.67 -0.3 833,309 - Atu8139 0.78 +0.8 833,626 - Atu0834 0.12 -0.8
Or see this region's nucleotide sequence