Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0811 and Atu0812 are separated by 207 nucleotides Atu0812 and Atu0813 are separated by 52 nucleotides Atu0813 and Atu0814 are separated by 264 nucleotides
Atu0811: Atu0811 - malate:quinone oxidoreductase, at 810,350 to 811,984
Atu0811
Atu0812: Atu0812 - hypothetical protein, at 812,192 to 812,401
Atu0812
Atu0813: Atu0813 - hypothetical protein, at 812,454 to 812,876
Atu0813
Atu0814: Atu0814 - tRNA-Pro, at 813,141 to 813,217
Atu0814
Position (kb)
812
813 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7 at 811.250 kb on - strand, within Atu0811 at 811.250 kb on - strand, within Atu0811 at 811.253 kb on + strand, within Atu0811 at 811.253 kb on + strand, within Atu0811 at 811.304 kb on - strand, within Atu0811 at 811.316 kb on - strand, within Atu0811 at 811.429 kb on + strand, within Atu0811 at 811.514 kb on + strand, within Atu0811 at 811.577 kb on + strand, within Atu0811 at 811.582 kb on - strand, within Atu0811 at 811.582 kb on - strand, within Atu0811 at 811.582 kb on - strand, within Atu0811 at 811.585 kb on - strand, within Atu0811 at 811.631 kb on + strand, within Atu0811 at 811.641 kb on + strand, within Atu0811 at 811.752 kb on - strand, within Atu0811 at 811.808 kb on - strand, within Atu0811 at 811.852 kb on + strand at 811.860 kb on - strand at 811.936 kb on + strand at 811.944 kb on - strand at 811.979 kb on + strand at 811.979 kb on + strand at 811.987 kb on - strand at 812.010 kb on - strand at 812.096 kb on + strand at 812.104 kb on - strand at 812.122 kb on + strand at 812.122 kb on + strand at 812.194 kb on - strand at 812.270 kb on + strand, within Atu0812 at 812.344 kb on - strand, within Atu0812 at 812.346 kb on + strand, within Atu0812 at 812.346 kb on + strand, within Atu0812 at 812.431 kb on - strand at 812.431 kb on - strand at 812.433 kb on + strand at 812.433 kb on + strand at 812.447 kb on + strand at 812.466 kb on + strand at 812.508 kb on + strand, within Atu0813 at 812.516 kb on - strand, within Atu0813 at 812.566 kb on + strand, within Atu0813 at 812.566 kb on + strand, within Atu0813 at 812.566 kb on + strand, within Atu0813 at 812.566 kb on + strand, within Atu0813 at 812.566 kb on + strand, within Atu0813 at 812.566 kb on + strand, within Atu0813 at 812.566 kb on + strand, within Atu0813 at 812.566 kb on + strand, within Atu0813 at 812.574 kb on - strand, within Atu0813 at 812.574 kb on - strand, within Atu0813 at 812.574 kb on - strand, within Atu0813 at 812.574 kb on - strand, within Atu0813 at 812.574 kb on - strand, within Atu0813 at 812.574 kb on - strand, within Atu0813 at 812.574 kb on - strand, within Atu0813 at 812.597 kb on - strand, within Atu0813 at 812.628 kb on - strand, within Atu0813 at 812.638 kb on - strand, within Atu0813 at 812.676 kb on + strand, within Atu0813 at 812.676 kb on + strand, within Atu0813 at 812.676 kb on + strand, within Atu0813 at 812.676 kb on + strand, within Atu0813 at 812.676 kb on + strand, within Atu0813 at 812.684 kb on - strand, within Atu0813 at 812.684 kb on - strand, within Atu0813 at 812.684 kb on - strand, within Atu0813 at 812.684 kb on - strand, within Atu0813 at 812.717 kb on - strand, within Atu0813 at 812.781 kb on + strand, within Atu0813 at 812.811 kb on + strand, within Atu0813 at 812.849 kb on - strand at 812.882 kb on + strand at 812.949 kb on + strand at 812.952 kb on + strand at 813.145 kb on + strand at 813.145 kb on + strand at 813.145 kb on + strand at 813.218 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 811,250 - Atu0811 0.55 -0.5 811,250 - Atu0811 0.55 +0.1 811,253 + Atu0811 0.55 -0.2 811,253 + Atu0811 0.55 +2.2 811,304 - Atu0811 0.58 -0.2 811,316 - Atu0811 0.59 -1.4 811,429 + Atu0811 0.66 -0.4 811,514 + Atu0811 0.71 +0.1 811,577 + Atu0811 0.75 -0.1 811,582 - Atu0811 0.75 +0.9 811,582 - Atu0811 0.75 -0.4 811,582 - Atu0811 0.75 -0.9 811,585 - Atu0811 0.76 +0.4 811,631 + Atu0811 0.78 +0.2 811,641 + Atu0811 0.79 +0.6 811,752 - Atu0811 0.86 -1.9 811,808 - Atu0811 0.89 -2.2 811,852 + -0.1 811,860 - +0.2 811,936 + +0.7 811,944 - +0.1 811,979 + +0.3 811,979 + -1.9 811,987 - -0.0 812,010 - -0.2 812,096 + +0.2 812,104 - -1.1 812,122 + -0.4 812,122 + -0.7 812,194 - -0.6 812,270 + Atu0812 0.37 +1.0 812,344 - Atu0812 0.72 -0.4 812,346 + Atu0812 0.73 +1.2 812,346 + Atu0812 0.73 -0.6 812,431 - +0.2 812,431 - +1.7 812,433 + +1.6 812,433 + +1.0 812,447 + +0.7 812,466 + +2.6 812,508 + Atu0813 0.13 +4.1 812,516 - Atu0813 0.15 +5.4 812,566 + Atu0813 0.26 +5.6 812,566 + Atu0813 0.26 +5.5 812,566 + Atu0813 0.26 +5.4 812,566 + Atu0813 0.26 +3.7 812,566 + Atu0813 0.26 +5.1 812,566 + Atu0813 0.26 +5.0 812,566 + Atu0813 0.26 +5.9 812,566 + Atu0813 0.26 +5.1 812,574 - Atu0813 0.28 +5.6 812,574 - Atu0813 0.28 +5.7 812,574 - Atu0813 0.28 +6.2 812,574 - Atu0813 0.28 +6.2 812,574 - Atu0813 0.28 +4.5 812,574 - Atu0813 0.28 +5.5 812,574 - Atu0813 0.28 +5.4 812,597 - Atu0813 0.34 +3.4 812,628 - Atu0813 0.41 +5.6 812,638 - Atu0813 0.43 +5.8 812,676 + Atu0813 0.52 +5.3 812,676 + Atu0813 0.52 +5.6 812,676 + Atu0813 0.52 +5.4 812,676 + Atu0813 0.52 +5.5 812,676 + Atu0813 0.52 +5.8 812,684 - Atu0813 0.54 +4.1 812,684 - Atu0813 0.54 +4.3 812,684 - Atu0813 0.54 +4.3 812,684 - Atu0813 0.54 +4.5 812,717 - Atu0813 0.62 +2.1 812,781 + Atu0813 0.77 +6.4 812,811 + Atu0813 0.84 +7.3 812,849 - +6.5 812,882 + +6.5 812,949 + +3.8 812,952 + +1.3 813,145 + -0.6 813,145 + -2.0 813,145 + -0.6 813,218 + +0.3
Or see this region's nucleotide sequence