Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0362 and Atu0363 are separated by 171 nucleotides Atu0363 and Atu0364 are separated by 63 nucleotides
Atu0362: Atu0362 - S-adenosylmethionine synthetase, at 356,353 to 357,615
Atu0362
Atu0363: Atu0363 - putative tRNA (guanine-N(7)-)-methyltransferase, at 357,787 to 358,485
Atu0363
Atu0364: Atu0364 - ribokinase, at 358,549 to 359,448
Atu0364
Position (kb) 357
358
359 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 357.717 kb on + strand at 357.751 kb on + strand at 357.847 kb on + strand at 357.850 kb on - strand at 357.872 kb on - strand, within Atu0363 at 357.902 kb on - strand, within Atu0363 at 357.904 kb on + strand, within Atu0363 at 357.991 kb on + strand, within Atu0363 at 358.023 kb on + strand, within Atu0363 at 358.185 kb on + strand, within Atu0363 at 358.185 kb on + strand, within Atu0363 at 358.185 kb on + strand, within Atu0363 at 358.185 kb on + strand, within Atu0363 at 358.193 kb on - strand, within Atu0363 at 358.193 kb on - strand, within Atu0363 at 358.193 kb on - strand, within Atu0363 at 358.269 kb on + strand, within Atu0363 at 358.295 kb on - strand, within Atu0363 at 358.311 kb on - strand, within Atu0363 at 358.412 kb on - strand, within Atu0363 at 358.422 kb on - strand at 358.583 kb on + strand at 358.701 kb on - strand, within Atu0364 at 358.828 kb on - strand, within Atu0364 at 358.834 kb on + strand, within Atu0364 at 358.859 kb on + strand, within Atu0364 at 358.859 kb on + strand, within Atu0364 at 358.938 kb on + strand, within Atu0364 at 358.975 kb on - strand, within Atu0364 at 358.981 kb on + strand, within Atu0364 at 359.044 kb on - strand, within Atu0364 at 359.423 kb on - strand at 359.424 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 357,717 + +1.4 357,751 + -1.4 357,847 + +0.1 357,850 - +0.6 357,872 - Atu0363 0.12 -0.3 357,902 - Atu0363 0.16 +0.3 357,904 + Atu0363 0.17 -0.8 357,991 + Atu0363 0.29 -0.6 358,023 + Atu0363 0.34 +0.5 358,185 + Atu0363 0.57 -0.8 358,185 + Atu0363 0.57 -1.0 358,185 + Atu0363 0.57 -0.6 358,185 + Atu0363 0.57 +0.4 358,193 - Atu0363 0.58 +0.6 358,193 - Atu0363 0.58 +0.0 358,193 - Atu0363 0.58 -0.8 358,269 + Atu0363 0.69 +1.1 358,295 - Atu0363 0.73 +0.2 358,311 - Atu0363 0.75 -0.8 358,412 - Atu0363 0.89 -0.4 358,422 - +0.6 358,583 + -2.8 358,701 - Atu0364 0.17 +0.0 358,828 - Atu0364 0.31 +1.5 358,834 + Atu0364 0.32 +0.0 358,859 + Atu0364 0.34 -0.5 358,859 + Atu0364 0.34 -0.9 358,938 + Atu0364 0.43 -0.1 358,975 - Atu0364 0.47 -0.5 358,981 + Atu0364 0.48 -0.3 359,044 - Atu0364 0.55 +0.0 359,423 - -0.2 359,424 - +0.3
Or see this region's nucleotide sequence