Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0145 and Atu0146 overlap by 4 nucleotides Atu0146 and Atu0147 are separated by 38 nucleotides Atu0147 and Atu0148 are separated by 83 nucleotides
Atu0145: Atu0145 - glutamate synthase large subunit, at 148,937 to 154,285
Atu0145
Atu0146: Atu0146 - sulfite reductase (NADPH) flavoprotein alpha-component, at 154,282 to 156,051
Atu0146
Atu0147: Atu0147 - hypothetical protein, at 156,090 to 156,596
Atu0147
Atu0148: Atu0148 - Atu0148-1 mutant of a conserved hypothetical protein, at 156,680 to 157,276
Atu0148
Position (kb) 154
155
156
157 Strain fitness (log2 ratio) -2
-1
0
1
2 at 153.302 kb on + strand, within Atu0145 at 153.318 kb on + strand, within Atu0145 at 153.401 kb on + strand, within Atu0145 at 153.428 kb on + strand, within Atu0145 at 153.512 kb on + strand, within Atu0145 at 153.559 kb on + strand, within Atu0145 at 153.559 kb on + strand, within Atu0145 at 153.624 kb on - strand, within Atu0145 at 153.754 kb on - strand at 153.776 kb on - strand at 153.805 kb on + strand at 153.830 kb on + strand at 153.888 kb on - strand at 153.888 kb on - strand at 153.890 kb on - strand at 153.898 kb on - strand at 153.900 kb on - strand at 153.907 kb on + strand at 153.910 kb on - strand at 153.910 kb on - strand at 153.944 kb on - strand at 153.944 kb on - strand at 153.953 kb on - strand at 154.012 kb on - strand at 154.026 kb on - strand at 154.107 kb on - strand at 154.109 kb on + strand at 154.109 kb on + strand at 154.152 kb on + strand at 154.174 kb on - strand at 154.223 kb on - strand at 154.241 kb on + strand at 154.245 kb on - strand at 154.273 kb on - strand at 154.275 kb on + strand at 154.278 kb on - strand at 154.325 kb on - strand at 154.557 kb on - strand, within Atu0146 at 154.654 kb on + strand, within Atu0146 at 154.757 kb on + strand, within Atu0146 at 154.840 kb on + strand, within Atu0146 at 154.848 kb on - strand, within Atu0146 at 154.953 kb on + strand, within Atu0146 at 154.953 kb on + strand, within Atu0146 at 154.994 kb on + strand, within Atu0146 at 155.012 kb on - strand, within Atu0146 at 155.037 kb on + strand, within Atu0146 at 155.078 kb on + strand, within Atu0146 at 155.124 kb on - strand, within Atu0146 at 155.275 kb on - strand, within Atu0146 at 155.276 kb on - strand, within Atu0146 at 155.335 kb on - strand, within Atu0146 at 155.345 kb on - strand, within Atu0146 at 155.388 kb on + strand, within Atu0146 at 155.408 kb on + strand, within Atu0146 at 155.423 kb on + strand, within Atu0146 at 155.478 kb on + strand, within Atu0146 at 155.535 kb on + strand, within Atu0146 at 155.542 kb on + strand, within Atu0146 at 155.548 kb on - strand, within Atu0146 at 155.647 kb on - strand, within Atu0146 at 155.671 kb on + strand, within Atu0146 at 155.856 kb on - strand, within Atu0146 at 155.876 kb on + strand at 155.876 kb on + strand at 155.913 kb on + strand at 155.952 kb on - strand at 156.053 kb on - strand at 156.081 kb on + strand at 156.095 kb on + strand at 156.184 kb on + strand, within Atu0147 at 156.240 kb on + strand, within Atu0147 at 156.272 kb on + strand, within Atu0147 at 156.435 kb on - strand, within Atu0147 at 156.530 kb on + strand, within Atu0147 at 156.665 kb on + strand at 156.759 kb on - strand, within Atu0148 at 156.841 kb on - strand, within Atu0148
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 153,302 + Atu0145 0.82 +0.2 153,318 + Atu0145 0.82 -0.0 153,401 + Atu0145 0.83 -1.0 153,428 + Atu0145 0.84 -1.0 153,512 + Atu0145 0.86 -0.4 153,559 + Atu0145 0.86 -0.3 153,559 + Atu0145 0.86 +0.0 153,624 - Atu0145 0.88 -0.8 153,754 - -0.3 153,776 - -0.3 153,805 + +0.3 153,830 + -0.4 153,888 - +0.3 153,888 - -0.0 153,890 - -1.1 153,898 - +0.9 153,900 - +1.7 153,907 + +0.1 153,910 - -2.4 153,910 - -0.1 153,944 - +0.1 153,944 - +0.8 153,953 - -0.9 154,012 - +0.1 154,026 - +0.5 154,107 - -0.2 154,109 + +0.1 154,109 + +0.9 154,152 + +0.5 154,174 - -0.2 154,223 - +0.1 154,241 + +0.8 154,245 - +2.0 154,273 - -0.1 154,275 + -1.7 154,278 - -0.2 154,325 - +0.1 154,557 - Atu0146 0.16 +0.3 154,654 + Atu0146 0.21 -0.3 154,757 + Atu0146 0.27 -0.2 154,840 + Atu0146 0.32 +0.8 154,848 - Atu0146 0.32 -0.0 154,953 + Atu0146 0.38 -0.1 154,953 + Atu0146 0.38 -1.4 154,994 + Atu0146 0.40 -0.8 155,012 - Atu0146 0.41 +0.3 155,037 + Atu0146 0.43 +0.5 155,078 + Atu0146 0.45 +0.3 155,124 - Atu0146 0.48 -0.3 155,275 - Atu0146 0.56 +0.9 155,276 - Atu0146 0.56 +0.0 155,335 - Atu0146 0.59 +0.7 155,345 - Atu0146 0.60 -0.3 155,388 + Atu0146 0.62 -0.8 155,408 + Atu0146 0.64 -0.7 155,423 + Atu0146 0.64 -0.1 155,478 + Atu0146 0.68 -0.3 155,535 + Atu0146 0.71 -0.8 155,542 + Atu0146 0.71 -0.1 155,548 - Atu0146 0.72 -0.7 155,647 - Atu0146 0.77 -0.1 155,671 + Atu0146 0.78 +0.4 155,856 - Atu0146 0.89 +1.1 155,876 + -0.3 155,876 + +0.7 155,913 + +0.1 155,952 - -0.3 156,053 - +0.0 156,081 + +0.0 156,095 + -0.4 156,184 + Atu0147 0.19 -0.8 156,240 + Atu0147 0.30 -0.9 156,272 + Atu0147 0.36 +0.7 156,435 - Atu0147 0.68 +0.1 156,530 + Atu0147 0.87 +1.1 156,665 + +0.0 156,759 - Atu0148 0.13 +2.0 156,841 - Atu0148 0.27 +0.7
Or see this region's nucleotide sequence