Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0136 and Atu0137 overlap by 4 nucleotides Atu0137 and Atu0138 are separated by 115 nucleotides
Atu0136: Atu0136 - adenosine deaminase, at 139,319 to 140,296
Atu0136
Atu0137: Atu0137 - phosphopentomutase, at 140,293 to 141,513
Atu0137
Atu0138: Atu0138 - surfeit 1, at 141,629 to 142,459
Atu0138
Position (kb) 140
141
142 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 139.306 kb on + strand at 139.335 kb on + strand at 139.426 kb on - strand, within Atu0136 at 139.452 kb on - strand, within Atu0136 at 139.518 kb on - strand, within Atu0136 at 139.618 kb on + strand, within Atu0136 at 139.626 kb on - strand, within Atu0136 at 139.639 kb on + strand, within Atu0136 at 139.727 kb on + strand, within Atu0136 at 139.837 kb on + strand, within Atu0136 at 139.985 kb on - strand, within Atu0136 at 140.114 kb on - strand, within Atu0136 at 140.130 kb on + strand, within Atu0136 at 140.250 kb on - strand at 140.335 kb on - strand at 140.445 kb on - strand, within Atu0137 at 140.476 kb on - strand, within Atu0137 at 140.513 kb on - strand, within Atu0137 at 140.574 kb on + strand, within Atu0137 at 140.661 kb on - strand, within Atu0137 at 140.749 kb on + strand, within Atu0137 at 140.749 kb on + strand, within Atu0137 at 140.821 kb on - strand, within Atu0137 at 140.888 kb on - strand, within Atu0137 at 140.921 kb on + strand, within Atu0137 at 141.030 kb on + strand, within Atu0137 at 141.116 kb on + strand, within Atu0137 at 141.124 kb on - strand, within Atu0137 at 141.370 kb on + strand, within Atu0137 at 141.385 kb on - strand, within Atu0137 at 141.443 kb on + strand at 141.574 kb on - strand at 141.726 kb on + strand, within Atu0138 at 141.734 kb on - strand, within Atu0138 at 141.736 kb on + strand, within Atu0138 at 141.795 kb on + strand, within Atu0138 at 141.916 kb on + strand, within Atu0138 at 141.970 kb on + strand, within Atu0138 at 142.003 kb on + strand, within Atu0138 at 142.044 kb on - strand, within Atu0138 at 142.106 kb on + strand, within Atu0138 at 142.106 kb on + strand, within Atu0138 at 142.119 kb on - strand, within Atu0138 at 142.124 kb on + strand, within Atu0138 at 142.183 kb on + strand, within Atu0138 at 142.245 kb on - strand, within Atu0138 at 142.255 kb on + strand, within Atu0138 at 142.255 kb on + strand, within Atu0138 at 142.263 kb on - strand, within Atu0138 at 142.263 kb on - strand, within Atu0138 at 142.290 kb on + strand, within Atu0138 at 142.500 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 139,306 + -0.8 139,335 + +0.2 139,426 - Atu0136 0.11 +0.4 139,452 - Atu0136 0.14 +0.2 139,518 - Atu0136 0.20 -0.9 139,618 + Atu0136 0.31 +0.3 139,626 - Atu0136 0.31 +1.1 139,639 + Atu0136 0.33 +0.0 139,727 + Atu0136 0.42 -0.0 139,837 + Atu0136 0.53 -1.1 139,985 - Atu0136 0.68 -0.2 140,114 - Atu0136 0.81 -0.1 140,130 + Atu0136 0.83 -0.2 140,250 - +0.1 140,335 - -2.6 140,445 - Atu0137 0.12 -1.8 140,476 - Atu0137 0.15 -1.5 140,513 - Atu0137 0.18 -1.2 140,574 + Atu0137 0.23 -1.1 140,661 - Atu0137 0.30 -2.7 140,749 + Atu0137 0.37 -1.4 140,749 + Atu0137 0.37 -3.0 140,821 - Atu0137 0.43 -1.2 140,888 - Atu0137 0.49 -0.9 140,921 + Atu0137 0.51 +1.6 141,030 + Atu0137 0.60 -2.3 141,116 + Atu0137 0.67 -2.6 141,124 - Atu0137 0.68 -1.0 141,370 + Atu0137 0.88 -0.6 141,385 - Atu0137 0.89 -1.4 141,443 + -1.1 141,574 - -1.2 141,726 + Atu0138 0.12 +1.3 141,734 - Atu0138 0.13 +0.9 141,736 + Atu0138 0.13 +1.6 141,795 + Atu0138 0.20 +0.1 141,916 + Atu0138 0.35 +1.6 141,970 + Atu0138 0.41 +1.2 142,003 + Atu0138 0.45 +1.4 142,044 - Atu0138 0.50 +0.2 142,106 + Atu0138 0.57 +1.1 142,106 + Atu0138 0.57 +0.4 142,119 - Atu0138 0.59 +0.6 142,124 + Atu0138 0.60 +1.7 142,183 + Atu0138 0.67 +0.4 142,245 - Atu0138 0.74 +0.5 142,255 + Atu0138 0.75 +0.5 142,255 + Atu0138 0.75 +0.5 142,263 - Atu0138 0.76 +1.4 142,263 - Atu0138 0.76 +0.1 142,290 + Atu0138 0.80 +1.6 142,500 + -0.9
Or see this region's nucleotide sequence