Strain Fitness in Agrobacterium fabrum C58 around Atu0122

Experiment: R2A with Vancomycin 0.009 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAtu0121 and Atu0122 are separated by 90 nucleotidesAtu0122 and Atu0123 are separated by 306 nucleotidesAtu0123 and Atu0124 are separated by 221 nucleotides Atu0121: Atu0121 - molecular chaperone, DnaJ family, at 124,981 to 126,114 Atu0121 Atu0122: Atu0122 - DNAK Protein, at 126,205 to 128,106 Atu0122 Atu0123: Atu0123 - tRNA-Ser, at 128,413 to 128,502 Atu0123 Atu0124: Atu0124 - hypothetical protein, at 128,724 to 129,041 Atu0124 Position (kb) 126 127 128 129Strain fitness (log2 ratio) -1 0 1 2at 128.235 kb on + strandat 128.235 kb on + strandat 128.243 kb on - strandat 128.243 kb on - strandat 128.243 kb on - strandat 128.281 kb on - strandat 128.291 kb on - strandat 128.393 kb on - strandat 128.407 kb on + strandat 128.820 kb on + strand, within Atu0124at 128.862 kb on - strand, within Atu0124at 128.873 kb on - strand, within Atu0124at 128.987 kb on - strand, within Atu0124at 128.987 kb on - strand, within Atu0124at 129.026 kb on - strandat 129.060 kb on - strandat 129.067 kb on + strandat 129.083 kb on - strandat 129.094 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM
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128,235 + +0.1
128,235 + +0.6
128,243 - +0.8
128,243 - +2.0
128,243 - +0.6
128,281 - -0.5
128,291 - -0.4
128,393 - +1.3
128,407 + -0.1
128,820 + Atu0124 0.30 +1.1
128,862 - Atu0124 0.43 +2.0
128,873 - Atu0124 0.47 +2.4
128,987 - Atu0124 0.83 +0.7
128,987 - Atu0124 0.83 +0.8
129,026 - -1.0
129,060 - +1.3
129,067 + +0.2
129,083 - +0.4
129,094 - -0.4

Or see this region's nucleotide sequence