Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0118 and Atu0119 are separated by 146 nucleotides Atu0119 and Atu0120 are separated by 13 nucleotides Atu0120 and Atu0121 are separated by 68 nucleotides Atu0121 and Atu0122 are separated by 90 nucleotides
Atu0118: Atu0118 - hypothetical protein, at 122,483 to 123,988
Atu0118
Atu0119: Atu0119 - hypothetical protein, at 124,135 to 124,347
Atu0119
Atu0120: Atu0120 - hypothetical protein, at 124,361 to 124,912
Atu0120
Atu0121: Atu0121 - molecular chaperone, DnaJ family, at 124,981 to 126,114
Atu0121
Atu0122: Atu0122 - DNAK Protein, at 126,205 to 128,106
Atu0122
Position (kb) 124
125
126
127 Strain fitness (log2 ratio) -2
-1
0
1
2 at 124.105 kb on - strand at 124.112 kb on - strand at 124.112 kb on - strand at 124.129 kb on + strand at 124.148 kb on + strand at 124.210 kb on - strand, within Atu0119 at 124.482 kb on - strand, within Atu0120 at 124.496 kb on + strand, within Atu0120 at 124.522 kb on + strand, within Atu0120 at 124.598 kb on + strand, within Atu0120 at 124.598 kb on + strand, within Atu0120 at 124.598 kb on + strand, within Atu0120 at 124.598 kb on + strand, within Atu0120 at 124.606 kb on - strand, within Atu0120 at 124.606 kb on - strand, within Atu0120 at 124.659 kb on + strand, within Atu0120 at 124.731 kb on - strand, within Atu0120 at 124.754 kb on - strand, within Atu0120 at 124.781 kb on - strand, within Atu0120 at 124.884 kb on + strand at 124.884 kb on + strand at 125.063 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 124,105 - -0.2 124,112 - -0.3 124,112 - +0.0 124,129 + +0.3 124,148 + +0.2 124,210 - Atu0119 0.35 +0.8 124,482 - Atu0120 0.22 +0.1 124,496 + Atu0120 0.24 +0.3 124,522 + Atu0120 0.29 +0.1 124,598 + Atu0120 0.43 -0.1 124,598 + Atu0120 0.43 -0.2 124,598 + Atu0120 0.43 -1.5 124,598 + Atu0120 0.43 -0.1 124,606 - Atu0120 0.44 -0.2 124,606 - Atu0120 0.44 -0.3 124,659 + Atu0120 0.54 +0.3 124,731 - Atu0120 0.67 +0.0 124,754 - Atu0120 0.71 +0.5 124,781 - Atu0120 0.76 +0.1 124,884 + -2.1 124,884 + -0.0 125,063 - +2.6
Or see this region's nucleotide sequence