Experiment: R2A with Phosphomycin 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0167 and Atu0168 are separated by 87 nucleotides Atu0168 and Atu0169 are separated by 44 nucleotides Atu0169 and Atu0170 overlap by 4 nucleotides Atu0170 and Atu0171 are separated by 283 nucleotides
Atu0167: Atu0167 - transcriptional regulator, AraC family, at 171,256 to 172,296
Atu0167
Atu0168: Atu0168 - guanylate kinase, at 172,384 to 172,935
Atu0168
Atu0169: Atu0169 - hypothetical protein, at 172,980 to 174,116
Atu0169
Atu0170: Atu0170 - hypothetical protein, at 174,113 to 174,802
Atu0170
Atu0171: Atu0171 - ABC transporter, membrane spanning protein (phosphonate), at 175,086 to 176,435
Atu0171
Position (kb) 172
173
174
175 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 172.028 kb on - strand, within Atu0167 at 172.077 kb on - strand, within Atu0167 at 172.149 kb on + strand, within Atu0167 at 172.149 kb on + strand, within Atu0167 at 172.225 kb on + strand at 172.381 kb on + strand at 172.401 kb on - strand at 172.509 kb on + strand, within Atu0168 at 172.540 kb on + strand, within Atu0168 at 172.554 kb on + strand, within Atu0168 at 172.554 kb on + strand, within Atu0168 at 172.554 kb on + strand, within Atu0168 at 172.562 kb on - strand, within Atu0168 at 172.646 kb on - strand, within Atu0168 at 172.713 kb on + strand, within Atu0168 at 172.812 kb on + strand, within Atu0168 at 173.057 kb on - strand at 173.155 kb on + strand, within Atu0169 at 173.163 kb on - strand, within Atu0169 at 173.315 kb on - strand, within Atu0169 at 173.316 kb on + strand, within Atu0169 at 173.383 kb on - strand, within Atu0169 at 173.461 kb on - strand, within Atu0169 at 173.611 kb on + strand, within Atu0169 at 173.617 kb on - strand, within Atu0169 at 173.617 kb on - strand, within Atu0169 at 173.656 kb on + strand, within Atu0169 at 173.830 kb on + strand, within Atu0169 at 173.840 kb on + strand, within Atu0169 at 173.877 kb on + strand, within Atu0169 at 173.923 kb on + strand, within Atu0169 at 173.923 kb on + strand, within Atu0169 at 173.923 kb on + strand, within Atu0169 at 173.923 kb on + strand, within Atu0169 at 173.931 kb on - strand, within Atu0169 at 173.931 kb on - strand, within Atu0169 at 174.029 kb on - strand at 174.045 kb on + strand at 174.053 kb on - strand at 174.091 kb on + strand at 174.099 kb on - strand at 174.109 kb on - strand at 174.310 kb on - strand, within Atu0170 at 174.347 kb on + strand, within Atu0170 at 174.347 kb on + strand, within Atu0170 at 174.347 kb on + strand, within Atu0170 at 174.355 kb on - strand, within Atu0170 at 174.419 kb on + strand, within Atu0170 at 174.427 kb on - strand, within Atu0170 at 174.429 kb on + strand, within Atu0170 at 174.437 kb on - strand, within Atu0170 at 174.623 kb on + strand, within Atu0170 at 174.631 kb on - strand, within Atu0170 at 174.719 kb on - strand, within Atu0170 at 174.748 kb on - strand at 174.883 kb on - strand at 174.980 kb on - strand at 174.995 kb on + strand at 174.998 kb on - strand at 175.003 kb on - strand at 175.010 kb on + strand at 175.010 kb on + strand at 175.028 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Phosphomycin 50 mM remove 172,028 - Atu0167 0.74 -0.3 172,077 - Atu0167 0.79 +0.1 172,149 + Atu0167 0.86 +0.4 172,149 + Atu0167 0.86 -0.4 172,225 + -0.3 172,381 + +0.1 172,401 - -1.0 172,509 + Atu0168 0.23 -0.1 172,540 + Atu0168 0.28 +0.4 172,554 + Atu0168 0.31 -0.1 172,554 + Atu0168 0.31 +0.9 172,554 + Atu0168 0.31 +0.4 172,562 - Atu0168 0.32 +0.4 172,646 - Atu0168 0.47 -0.5 172,713 + Atu0168 0.60 -1.0 172,812 + Atu0168 0.78 +0.5 173,057 - -4.0 173,155 + Atu0169 0.15 -1.9 173,163 - Atu0169 0.16 -4.0 173,315 - Atu0169 0.29 -4.8 173,316 + Atu0169 0.30 -6.0 173,383 - Atu0169 0.35 -3.8 173,461 - Atu0169 0.42 +0.2 173,611 + Atu0169 0.55 -5.7 173,617 - Atu0169 0.56 -3.9 173,617 - Atu0169 0.56 -5.7 173,656 + Atu0169 0.59 -4.4 173,830 + Atu0169 0.75 -5.0 173,840 + Atu0169 0.76 -2.9 173,877 + Atu0169 0.79 -2.5 173,923 + Atu0169 0.83 -2.3 173,923 + Atu0169 0.83 -4.1 173,923 + Atu0169 0.83 -3.7 173,923 + Atu0169 0.83 -0.5 173,931 - Atu0169 0.84 -4.2 173,931 - Atu0169 0.84 -4.7 174,029 - -0.2 174,045 + -1.8 174,053 - -3.8 174,091 + -1.0 174,099 - -3.0 174,109 - -4.9 174,310 - Atu0170 0.29 +0.7 174,347 + Atu0170 0.34 -1.2 174,347 + Atu0170 0.34 -0.7 174,347 + Atu0170 0.34 -1.4 174,355 - Atu0170 0.35 +0.7 174,419 + Atu0170 0.44 -0.5 174,427 - Atu0170 0.46 -0.0 174,429 + Atu0170 0.46 -0.1 174,437 - Atu0170 0.47 -2.6 174,623 + Atu0170 0.74 -0.9 174,631 - Atu0170 0.75 -2.1 174,719 - Atu0170 0.88 +0.1 174,748 - -3.2 174,883 - +0.6 174,980 - -1.0 174,995 + -0.4 174,998 - -0.3 175,003 - -0.1 175,010 + -0.3 175,010 + +0.2 175,028 - -2.3
Or see this region's nucleotide sequence