Experiment: R2A with Polymyxin B sulfate 0.00075 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4610 and Atu4611 are separated by 304 nucleotides Atu4611 and Atu4613 are separated by 236 nucleotides Atu4613 and Atu4614 are separated by 154 nucleotides
Atu4610: Atu4610 - sugar nucleotide epimerase/dehydratase, at 1,756,928 to 1,758,004
Atu4610
Atu4611: Atu4611 - transposase, at 1,758,309 to 1,758,584
Atu4611
Atu4613: Atu4613 - glycosyltransferase, at 1,758,821 to 1,761,184
Atu4613
Atu4614: Atu4614 - glycosyltransferase, at 1,761,339 to 1,762,220
Atu4614
Position (kb)
1758
1759
1760
1761
1762 Strain fitness (log2 ratio)
-8
-7
-6
-5
-4
-3
-2
-1
0
1 at 1757.835 kb on - strand, within Atu4610 at 1757.911 kb on - strand at 1757.926 kb on + strand at 1757.961 kb on - strand at 1757.961 kb on - strand at 1757.968 kb on + strand at 1758.184 kb on - strand at 1758.193 kb on - strand at 1758.204 kb on - strand at 1758.204 kb on - strand at 1758.246 kb on - strand at 1758.254 kb on - strand at 1758.268 kb on + strand at 1758.271 kb on - strand at 1758.271 kb on - strand at 1758.276 kb on - strand at 1758.345 kb on + strand, within Atu4611 at 1758.345 kb on + strand, within Atu4611 at 1758.350 kb on + strand, within Atu4611 at 1758.350 kb on + strand, within Atu4611 at 1758.358 kb on - strand, within Atu4611 at 1758.358 kb on - strand, within Atu4611 at 1758.358 kb on - strand, within Atu4611 at 1758.418 kb on - strand, within Atu4611 at 1758.418 kb on - strand, within Atu4611 at 1758.450 kb on - strand, within Atu4611 at 1758.617 kb on + strand at 1758.625 kb on - strand at 1758.625 kb on - strand at 1758.653 kb on + strand at 1758.671 kb on - strand at 1758.693 kb on + strand at 1758.693 kb on - strand at 1758.725 kb on - strand at 1758.751 kb on - strand at 1758.755 kb on - strand at 1758.819 kb on - strand at 1758.892 kb on + strand at 1759.115 kb on + strand, within Atu4613 at 1759.141 kb on + strand, within Atu4613 at 1759.141 kb on + strand, within Atu4613 at 1759.156 kb on + strand, within Atu4613 at 1759.368 kb on + strand, within Atu4613 at 1759.368 kb on + strand, within Atu4613 at 1759.368 kb on + strand, within Atu4613 at 1759.376 kb on - strand, within Atu4613 at 1759.385 kb on + strand, within Atu4613 at 1759.537 kb on + strand, within Atu4613 at 1759.545 kb on - strand, within Atu4613 at 1759.545 kb on - strand, within Atu4613 at 1759.550 kb on - strand, within Atu4613 at 1759.550 kb on - strand, within Atu4613 at 1759.551 kb on - strand, within Atu4613 at 1759.604 kb on - strand, within Atu4613 at 1759.641 kb on + strand, within Atu4613 at 1759.692 kb on - strand, within Atu4613 at 1759.701 kb on + strand, within Atu4613 at 1759.833 kb on + strand, within Atu4613 at 1759.861 kb on + strand, within Atu4613 at 1759.869 kb on - strand, within Atu4613 at 1759.869 kb on - strand, within Atu4613 at 1759.926 kb on - strand, within Atu4613 at 1760.168 kb on + strand, within Atu4613 at 1760.223 kb on + strand, within Atu4613 at 1760.320 kb on - strand, within Atu4613 at 1760.433 kb on - strand, within Atu4613 at 1760.466 kb on + strand, within Atu4613 at 1760.502 kb on + strand, within Atu4613 at 1760.510 kb on - strand, within Atu4613 at 1760.510 kb on - strand, within Atu4613 at 1760.511 kb on - strand, within Atu4613 at 1760.590 kb on - strand, within Atu4613 at 1760.595 kb on + strand, within Atu4613 at 1761.029 kb on - strand at 1761.244 kb on + strand at 1761.385 kb on + strand at 1761.390 kb on + strand at 1761.398 kb on - strand at 1761.470 kb on + strand, within Atu4614 at 1761.540 kb on - strand, within Atu4614 at 1761.562 kb on + strand, within Atu4614 at 1761.563 kb on - strand, within Atu4614 at 1761.570 kb on - strand, within Atu4614 at 1761.765 kb on - strand, within Atu4614 at 1761.770 kb on - strand, within Atu4614 at 1761.770 kb on - strand, within Atu4614 at 1761.821 kb on - strand, within Atu4614 at 1761.903 kb on + strand, within Atu4614 at 1761.916 kb on + strand, within Atu4614 at 1762.006 kb on + strand, within Atu4614 at 1762.014 kb on - strand, within Atu4614 at 1762.079 kb on + strand, within Atu4614 at 1762.086 kb on + strand, within Atu4614 at 1762.112 kb on + strand, within Atu4614 at 1762.112 kb on + strand, within Atu4614 at 1762.168 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.00075 mM remove 1,757,835 - Atu4610 0.84 -4.0 1,757,911 - -4.4 1,757,926 + -4.2 1,757,961 - -4.1 1,757,961 - -3.2 1,757,968 + -1.9 1,758,184 - -0.6 1,758,193 - -0.6 1,758,204 - -0.2 1,758,204 - -0.2 1,758,246 - -0.2 1,758,254 - +0.3 1,758,268 + -0.1 1,758,271 - -0.1 1,758,271 - -0.6 1,758,276 - -0.9 1,758,345 + Atu4611 0.13 -0.3 1,758,345 + Atu4611 0.13 +1.7 1,758,350 + Atu4611 0.15 +1.0 1,758,350 + Atu4611 0.15 -0.6 1,758,358 - Atu4611 0.18 -0.2 1,758,358 - Atu4611 0.18 +0.0 1,758,358 - Atu4611 0.18 +0.1 1,758,418 - Atu4611 0.39 +0.6 1,758,418 - Atu4611 0.39 -0.3 1,758,450 - Atu4611 0.51 +0.7 1,758,617 + +0.4 1,758,625 - +0.0 1,758,625 - +0.3 1,758,653 + -0.6 1,758,671 - +0.1 1,758,693 + +0.3 1,758,693 - -0.9 1,758,725 - -0.3 1,758,751 - -0.2 1,758,755 - -1.0 1,758,819 - +0.7 1,758,892 + -3.3 1,759,115 + Atu4613 0.12 -6.5 1,759,141 + Atu4613 0.14 -3.2 1,759,141 + Atu4613 0.14 -2.3 1,759,156 + Atu4613 0.14 -2.9 1,759,368 + Atu4613 0.23 -6.4 1,759,368 + Atu4613 0.23 -3.3 1,759,368 + Atu4613 0.23 -2.8 1,759,376 - Atu4613 0.23 -2.1 1,759,385 + Atu4613 0.24 -2.6 1,759,537 + Atu4613 0.30 -1.1 1,759,545 - Atu4613 0.31 -5.2 1,759,545 - Atu4613 0.31 -6.4 1,759,550 - Atu4613 0.31 -4.7 1,759,550 - Atu4613 0.31 -4.8 1,759,551 - Atu4613 0.31 -5.5 1,759,604 - Atu4613 0.33 -5.9 1,759,641 + Atu4613 0.35 -5.1 1,759,692 - Atu4613 0.37 -3.4 1,759,701 + Atu4613 0.37 -6.1 1,759,833 + Atu4613 0.43 -5.8 1,759,861 + Atu4613 0.44 -5.8 1,759,869 - Atu4613 0.44 -5.3 1,759,869 - Atu4613 0.44 -2.1 1,759,926 - Atu4613 0.47 -6.5 1,760,168 + Atu4613 0.57 -4.1 1,760,223 + Atu4613 0.59 -4.5 1,760,320 - Atu4613 0.63 -1.2 1,760,433 - Atu4613 0.68 -5.2 1,760,466 + Atu4613 0.70 -5.5 1,760,502 + Atu4613 0.71 -4.7 1,760,510 - Atu4613 0.71 -5.6 1,760,510 - Atu4613 0.71 -5.9 1,760,511 - Atu4613 0.71 -5.0 1,760,590 - Atu4613 0.75 -3.8 1,760,595 + Atu4613 0.75 -8.3 1,761,029 - -4.4 1,761,244 + -1.5 1,761,385 + -3.6 1,761,390 + -3.2 1,761,398 - -4.5 1,761,470 + Atu4614 0.15 -5.4 1,761,540 - Atu4614 0.23 -3.6 1,761,562 + Atu4614 0.25 -4.5 1,761,563 - Atu4614 0.25 -5.1 1,761,570 - Atu4614 0.26 -3.7 1,761,765 - Atu4614 0.48 -3.1 1,761,770 - Atu4614 0.49 -2.3 1,761,770 - Atu4614 0.49 -6.0 1,761,821 - Atu4614 0.55 -5.7 1,761,903 + Atu4614 0.64 -4.5 1,761,916 + Atu4614 0.65 -1.3 1,762,006 + Atu4614 0.76 -4.5 1,762,014 - Atu4614 0.77 -3.0 1,762,079 + Atu4614 0.84 -5.6 1,762,086 + Atu4614 0.85 -1.7 1,762,112 + Atu4614 0.88 -2.7 1,762,112 + Atu4614 0.88 -3.0 1,762,168 - -4.6
Or see this region's nucleotide sequence