Experiment: R2A with Polymyxin B sulfate 0.00075 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0749 and Atu0750 are separated by 5 nucleotides Atu0750 and Atu0751 overlap by 4 nucleotides Atu0751 and Atu0752 are separated by 112 nucleotides
Atu0749: Atu0749 - biotin synthesis BioY protein, at 747,148 to 747,711
Atu0749
Atu0750: Atu0750 - ABC transporter, membrane spanning protein, at 747,717 to 748,349
Atu0750
Atu0751: Atu0751 - ABC transporter, nucleotide binding/ATPase protein (cobalt), at 748,346 to 749,026
Atu0751
Atu0752: Atu0752 - hypothetical protein, at 749,139 to 750,335
Atu0752
Position (kb) 748
749
750 Strain fitness (log2 ratio) -2
-1
0
1 at 747.418 kb on + strand, within Atu0749 at 747.509 kb on - strand, within Atu0749 at 747.588 kb on - strand, within Atu0749 at 747.603 kb on + strand, within Atu0749 at 747.659 kb on - strand at 747.747 kb on + strand at 747.747 kb on + strand at 747.839 kb on - strand, within Atu0750 at 747.889 kb on - strand, within Atu0750 at 747.889 kb on - strand, within Atu0750 at 747.891 kb on - strand, within Atu0750 at 747.928 kb on - strand, within Atu0750 at 747.952 kb on + strand, within Atu0750 at 747.960 kb on - strand, within Atu0750 at 747.970 kb on - strand, within Atu0750 at 747.992 kb on + strand, within Atu0750 at 748.000 kb on - strand, within Atu0750 at 748.000 kb on - strand, within Atu0750 at 748.029 kb on + strand, within Atu0750 at 748.050 kb on - strand, within Atu0750 at 748.084 kb on + strand, within Atu0750 at 748.113 kb on + strand, within Atu0750 at 748.121 kb on - strand, within Atu0750 at 748.142 kb on + strand, within Atu0750 at 748.142 kb on + strand, within Atu0750 at 748.199 kb on + strand, within Atu0750 at 748.199 kb on + strand, within Atu0750 at 748.207 kb on - strand, within Atu0750 at 748.207 kb on - strand, within Atu0750 at 748.207 kb on - strand, within Atu0750 at 748.207 kb on - strand, within Atu0750 at 748.209 kb on + strand, within Atu0750 at 748.209 kb on + strand, within Atu0750 at 748.217 kb on - strand, within Atu0750 at 748.217 kb on - strand, within Atu0750 at 748.259 kb on + strand, within Atu0750 at 748.267 kb on - strand, within Atu0750 at 748.296 kb on - strand at 748.360 kb on + strand at 748.360 kb on + strand at 748.360 kb on + strand at 748.368 kb on - strand at 748.374 kb on + strand at 748.436 kb on + strand, within Atu0751 at 748.505 kb on - strand, within Atu0751 at 748.627 kb on + strand, within Atu0751 at 748.835 kb on + strand, within Atu0751 at 748.864 kb on + strand, within Atu0751 at 748.913 kb on - strand, within Atu0751 at 748.935 kb on + strand, within Atu0751 at 748.954 kb on + strand, within Atu0751 at 749.035 kb on - strand at 749.052 kb on + strand at 749.060 kb on - strand at 749.230 kb on - strand at 749.246 kb on - strand at 749.284 kb on + strand, within Atu0752 at 749.284 kb on + strand, within Atu0752 at 749.475 kb on - strand, within Atu0752 at 749.580 kb on + strand, within Atu0752 at 749.616 kb on + strand, within Atu0752 at 749.618 kb on + strand, within Atu0752 at 749.626 kb on - strand, within Atu0752 at 749.699 kb on - strand, within Atu0752 at 749.719 kb on - strand, within Atu0752 at 749.773 kb on + strand, within Atu0752 at 749.853 kb on + strand, within Atu0752 at 749.942 kb on - strand, within Atu0752 at 749.963 kb on + strand, within Atu0752 at 750.000 kb on + strand, within Atu0752 at 750.018 kb on + strand, within Atu0752 at 750.018 kb on - strand, within Atu0752
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.00075 mM remove 747,418 + Atu0749 0.48 +0.2 747,509 - Atu0749 0.64 -0.1 747,588 - Atu0749 0.78 +0.4 747,603 + Atu0749 0.81 +1.0 747,659 - +0.3 747,747 + +0.9 747,747 + +0.1 747,839 - Atu0750 0.19 +0.3 747,889 - Atu0750 0.27 -0.5 747,889 - Atu0750 0.27 +0.8 747,891 - Atu0750 0.27 -0.0 747,928 - Atu0750 0.33 -0.3 747,952 + Atu0750 0.37 +0.5 747,960 - Atu0750 0.38 +0.1 747,970 - Atu0750 0.40 -0.1 747,992 + Atu0750 0.43 +0.2 748,000 - Atu0750 0.45 +0.2 748,000 - Atu0750 0.45 -0.2 748,029 + Atu0750 0.49 -0.6 748,050 - Atu0750 0.53 +0.5 748,084 + Atu0750 0.58 +1.3 748,113 + Atu0750 0.63 -0.1 748,121 - Atu0750 0.64 +0.8 748,142 + Atu0750 0.67 +0.7 748,142 + Atu0750 0.67 -0.4 748,199 + Atu0750 0.76 +0.1 748,199 + Atu0750 0.76 +0.2 748,207 - Atu0750 0.77 -0.7 748,207 - Atu0750 0.77 +1.7 748,207 - Atu0750 0.77 -0.7 748,207 - Atu0750 0.77 +0.1 748,209 + Atu0750 0.78 +0.3 748,209 + Atu0750 0.78 +0.2 748,217 - Atu0750 0.79 +0.4 748,217 - Atu0750 0.79 -0.0 748,259 + Atu0750 0.86 -1.4 748,267 - Atu0750 0.87 -1.1 748,296 - -0.2 748,360 + -0.1 748,360 + +0.4 748,360 + +0.2 748,368 - +0.6 748,374 + -1.6 748,436 + Atu0751 0.13 +0.1 748,505 - Atu0751 0.23 -0.1 748,627 + Atu0751 0.41 +0.4 748,835 + Atu0751 0.72 +0.2 748,864 + Atu0751 0.76 +0.4 748,913 - Atu0751 0.83 +0.5 748,935 + Atu0751 0.86 +0.5 748,954 + Atu0751 0.89 +0.5 749,035 - +0.1 749,052 + +0.0 749,060 - -0.2 749,230 - -0.4 749,246 - -0.7 749,284 + Atu0752 0.12 +0.1 749,284 + Atu0752 0.12 -0.3 749,475 - Atu0752 0.28 +0.3 749,580 + Atu0752 0.37 -2.7 749,616 + Atu0752 0.40 -0.2 749,618 + Atu0752 0.40 +0.0 749,626 - Atu0752 0.41 +0.1 749,699 - Atu0752 0.47 +0.2 749,719 - Atu0752 0.48 -0.1 749,773 + Atu0752 0.53 +1.1 749,853 + Atu0752 0.60 -0.8 749,942 - Atu0752 0.67 +0.1 749,963 + Atu0752 0.69 +0.6 750,000 + Atu0752 0.72 -0.2 750,018 + Atu0752 0.73 +0.1 750,018 - Atu0752 0.73 +0.1
Or see this region's nucleotide sequence