Experiment: R2A with Gentamicin 0.00125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2111 and Atu2112 are separated by 92 nucleotides Atu2112 and Atu2113 are separated by 247 nucleotides Atu2113 and Atu2114 overlap by 4 nucleotides
Atu2111: Atu2111 - sarcosine oxidase, at 2,075,243 to 2,076,430
Atu2111
Atu2112: Atu2112 - soluble lytic transglycosylase, at 2,076,523 to 2,077,062
Atu2112
Atu2113: Atu2113 - N-acetylmuramoyl-L-alanine amidase, at 2,077,310 to 2,078,089
Atu2113
Atu2114: Atu2114 - molecular chaperone, DnaJ family, at 2,078,086 to 2,078,823
Atu2114
Position (kb)
2077
2078
2079 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2076.387 kb on + strand at 2076.431 kb on + strand at 2076.467 kb on + strand at 2076.546 kb on - strand at 2076.612 kb on + strand, within Atu2112 at 2076.617 kb on + strand, within Atu2112 at 2076.617 kb on + strand, within Atu2112 at 2076.625 kb on - strand, within Atu2112 at 2076.627 kb on - strand, within Atu2112 at 2076.700 kb on - strand, within Atu2112 at 2076.711 kb on - strand, within Atu2112 at 2076.764 kb on - strand, within Atu2112 at 2076.769 kb on - strand, within Atu2112 at 2076.781 kb on + strand, within Atu2112 at 2076.802 kb on - strand, within Atu2112 at 2076.829 kb on + strand, within Atu2112 at 2076.846 kb on - strand, within Atu2112 at 2076.856 kb on - strand, within Atu2112 at 2076.856 kb on - strand, within Atu2112 at 2076.876 kb on + strand, within Atu2112 at 2076.957 kb on - strand, within Atu2112 at 2076.973 kb on - strand, within Atu2112 at 2076.973 kb on - strand, within Atu2112 at 2077.185 kb on + strand at 2077.333 kb on + strand at 2077.497 kb on - strand, within Atu2113 at 2077.584 kb on - strand, within Atu2113 at 2077.686 kb on - strand, within Atu2113 at 2077.961 kb on + strand, within Atu2113 at 2078.419 kb on + strand, within Atu2114 at 2078.474 kb on - strand, within Atu2114 at 2078.481 kb on - strand, within Atu2114 at 2078.503 kb on + strand, within Atu2114 at 2078.529 kb on - strand, within Atu2114 at 2078.702 kb on + strand, within Atu2114 at 2078.702 kb on + strand, within Atu2114 at 2078.710 kb on - strand, within Atu2114 at 2078.710 kb on - strand, within Atu2114 at 2078.750 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Gentamicin 0.00125 mM remove 2,076,387 + -0.5 2,076,431 + -0.1 2,076,467 + +0.7 2,076,546 - -0.3 2,076,612 + Atu2112 0.16 +0.2 2,076,617 + Atu2112 0.17 +0.3 2,076,617 + Atu2112 0.17 +0.1 2,076,625 - Atu2112 0.19 +1.8 2,076,627 - Atu2112 0.19 -2.3 2,076,700 - Atu2112 0.33 +0.0 2,076,711 - Atu2112 0.35 -0.0 2,076,764 - Atu2112 0.45 +0.5 2,076,769 - Atu2112 0.46 -0.3 2,076,781 + Atu2112 0.48 -0.2 2,076,802 - Atu2112 0.52 +0.1 2,076,829 + Atu2112 0.57 +0.0 2,076,846 - Atu2112 0.60 +0.3 2,076,856 - Atu2112 0.62 -0.4 2,076,856 - Atu2112 0.62 +0.0 2,076,876 + Atu2112 0.65 -0.1 2,076,957 - Atu2112 0.80 -0.1 2,076,973 - Atu2112 0.83 -0.3 2,076,973 - Atu2112 0.83 -0.2 2,077,185 + +0.5 2,077,333 + -0.3 2,077,497 - Atu2113 0.24 -0.8 2,077,584 - Atu2113 0.35 -2.9 2,077,686 - Atu2113 0.48 -1.3 2,077,961 + Atu2113 0.83 -2.3 2,078,419 + Atu2114 0.45 +0.8 2,078,474 - Atu2114 0.53 -0.8 2,078,481 - Atu2114 0.54 -1.9 2,078,503 + Atu2114 0.57 -0.3 2,078,529 - Atu2114 0.60 -1.0 2,078,702 + Atu2114 0.83 -0.4 2,078,702 + Atu2114 0.83 -0.8 2,078,710 - Atu2114 0.85 -1.3 2,078,710 - Atu2114 0.85 -0.9 2,078,750 - -0.8
Or see this region's nucleotide sequence