Experiment: R2A with Nalidixic 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2582 and Atu2583 are separated by 193 nucleotides Atu2583 and Atu2584 overlap by 4 nucleotides Atu2584 and Atu2585 overlap by 9 nucleotides
Atu2582: Atu2582 - UDP-glucose/GDP-mannose dehydrogenase, at 2,556,795 to 2,558,069
Atu2582
Atu2583: Atu2583 - glycosyltransferase, at 2,558,263 to 2,559,360
Atu2583
Atu2584: Atu2584 - hypothetical protein, at 2,559,357 to 2,560,100
Atu2584
Atu2585: Atu2585 - hypothetical protein, at 2,560,092 to 2,560,931
Atu2585
Position (kb)
2558
2559
2560 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2557.319 kb on + strand, within Atu2582 at 2557.363 kb on - strand, within Atu2582 at 2557.422 kb on - strand, within Atu2582 at 2557.573 kb on + strand, within Atu2582 at 2557.678 kb on - strand, within Atu2582 at 2557.680 kb on + strand, within Atu2582 at 2557.682 kb on + strand, within Atu2582 at 2557.698 kb on - strand, within Atu2582 at 2557.725 kb on - strand, within Atu2582 at 2557.758 kb on - strand, within Atu2582 at 2557.760 kb on + strand, within Atu2582 at 2557.760 kb on + strand, within Atu2582 at 2557.933 kb on - strand, within Atu2582 at 2558.058 kb on - strand at 2558.128 kb on - strand at 2558.138 kb on - strand at 2558.203 kb on + strand at 2558.247 kb on + strand at 2558.306 kb on - strand at 2558.373 kb on + strand, within Atu2583 at 2558.384 kb on + strand, within Atu2583 at 2558.389 kb on + strand, within Atu2583 at 2558.392 kb on - strand, within Atu2583 at 2558.392 kb on - strand, within Atu2583 at 2558.432 kb on - strand, within Atu2583 at 2558.437 kb on - strand, within Atu2583 at 2558.698 kb on + strand, within Atu2583 at 2558.705 kb on + strand, within Atu2583 at 2558.707 kb on + strand, within Atu2583 at 2558.786 kb on + strand, within Atu2583 at 2558.814 kb on + strand, within Atu2583 at 2558.822 kb on - strand, within Atu2583 at 2558.861 kb on + strand, within Atu2583 at 2558.861 kb on + strand, within Atu2583 at 2558.861 kb on + strand, within Atu2583 at 2558.861 kb on + strand, within Atu2583 at 2558.861 kb on + strand, within Atu2583 at 2558.861 kb on + strand, within Atu2583 at 2558.861 kb on + strand, within Atu2583 at 2558.861 kb on + strand, within Atu2583 at 2558.869 kb on - strand, within Atu2583 at 2558.869 kb on - strand, within Atu2583 at 2558.869 kb on - strand, within Atu2583 at 2558.869 kb on - strand, within Atu2583 at 2558.869 kb on - strand, within Atu2583 at 2558.869 kb on - strand, within Atu2583 at 2558.869 kb on - strand, within Atu2583 at 2558.869 kb on - strand, within Atu2583 at 2558.879 kb on - strand, within Atu2583 at 2558.886 kb on - strand, within Atu2583 at 2558.900 kb on - strand, within Atu2583 at 2559.072 kb on + strand, within Atu2583 at 2559.073 kb on + strand, within Atu2583 at 2559.112 kb on - strand, within Atu2583 at 2559.118 kb on + strand, within Atu2583 at 2559.118 kb on + strand, within Atu2583 at 2559.172 kb on + strand, within Atu2583 at 2559.229 kb on - strand, within Atu2583 at 2559.236 kb on - strand, within Atu2583 at 2559.244 kb on - strand, within Atu2583 at 2559.246 kb on + strand, within Atu2583 at 2559.361 kb on - strand at 2559.700 kb on - strand, within Atu2584 at 2559.710 kb on + strand, within Atu2584 at 2559.734 kb on - strand, within Atu2584 at 2559.848 kb on + strand, within Atu2584 at 2559.928 kb on + strand, within Atu2584 at 2559.943 kb on + strand, within Atu2584 at 2560.012 kb on + strand, within Atu2584 at 2560.022 kb on + strand, within Atu2584 at 2560.036 kb on + strand at 2560.074 kb on + strand at 2560.115 kb on + strand at 2560.128 kb on - strand at 2560.219 kb on - strand, within Atu2585 at 2560.225 kb on - strand, within Atu2585 at 2560.246 kb on + strand, within Atu2585 at 2560.275 kb on - strand, within Atu2585
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nalidixic 0.02 mM remove 2,557,319 + Atu2582 0.41 -3.4 2,557,363 - Atu2582 0.45 -1.3 2,557,422 - Atu2582 0.49 -2.4 2,557,573 + Atu2582 0.61 -2.4 2,557,678 - Atu2582 0.69 -1.4 2,557,680 + Atu2582 0.69 -1.6 2,557,682 + Atu2582 0.70 -2.3 2,557,698 - Atu2582 0.71 -4.1 2,557,725 - Atu2582 0.73 -2.2 2,557,758 - Atu2582 0.76 -1.5 2,557,760 + Atu2582 0.76 -1.4 2,557,760 + Atu2582 0.76 -1.1 2,557,933 - Atu2582 0.89 +2.6 2,558,058 - +1.2 2,558,128 - -1.1 2,558,138 - -1.0 2,558,203 + -0.2 2,558,247 + +0.1 2,558,306 - -1.9 2,558,373 + Atu2583 0.10 -2.1 2,558,384 + Atu2583 0.11 -3.1 2,558,389 + Atu2583 0.11 -2.7 2,558,392 - Atu2583 0.12 +1.0 2,558,392 - Atu2583 0.12 +0.2 2,558,432 - Atu2583 0.15 -3.0 2,558,437 - Atu2583 0.16 -1.1 2,558,698 + Atu2583 0.40 -0.3 2,558,705 + Atu2583 0.40 -1.0 2,558,707 + Atu2583 0.40 -3.4 2,558,786 + Atu2583 0.48 -2.7 2,558,814 + Atu2583 0.50 -1.5 2,558,822 - Atu2583 0.51 -3.3 2,558,861 + Atu2583 0.54 -1.7 2,558,861 + Atu2583 0.54 -1.7 2,558,861 + Atu2583 0.54 -0.6 2,558,861 + Atu2583 0.54 -3.6 2,558,861 + Atu2583 0.54 +0.6 2,558,861 + Atu2583 0.54 -2.7 2,558,861 + Atu2583 0.54 -1.4 2,558,861 + Atu2583 0.54 -2.4 2,558,869 - Atu2583 0.55 -1.1 2,558,869 - Atu2583 0.55 -2.5 2,558,869 - Atu2583 0.55 -1.9 2,558,869 - Atu2583 0.55 -1.7 2,558,869 - Atu2583 0.55 -2.3 2,558,869 - Atu2583 0.55 -0.8 2,558,869 - Atu2583 0.55 +0.2 2,558,869 - Atu2583 0.55 +1.0 2,558,879 - Atu2583 0.56 -1.5 2,558,886 - Atu2583 0.57 -2.4 2,558,900 - Atu2583 0.58 -2.4 2,559,072 + Atu2583 0.74 -3.5 2,559,073 + Atu2583 0.74 -0.4 2,559,112 - Atu2583 0.77 +0.4 2,559,118 + Atu2583 0.78 +0.6 2,559,118 + Atu2583 0.78 +0.2 2,559,172 + Atu2583 0.83 -2.1 2,559,229 - Atu2583 0.88 -1.1 2,559,236 - Atu2583 0.89 -3.4 2,559,244 - Atu2583 0.89 -2.1 2,559,246 + Atu2583 0.90 -1.4 2,559,361 - -0.2 2,559,700 - Atu2584 0.46 -0.7 2,559,710 + Atu2584 0.47 -3.3 2,559,734 - Atu2584 0.51 +0.6 2,559,848 + Atu2584 0.66 -3.3 2,559,928 + Atu2584 0.77 -1.7 2,559,943 + Atu2584 0.79 -0.6 2,560,012 + Atu2584 0.88 +0.6 2,560,022 + Atu2584 0.89 -3.1 2,560,036 + -1.4 2,560,074 + -2.5 2,560,115 + -2.3 2,560,128 - -2.9 2,560,219 - Atu2585 0.15 -1.4 2,560,225 - Atu2585 0.16 -1.5 2,560,246 + Atu2585 0.18 -0.6 2,560,275 - Atu2585 0.22 +2.6
Or see this region's nucleotide sequence