Experiment: R2A with Nalidixic 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1604 and Atu1605 are separated by 31 nucleotides Atu1605 and Atu1606 are separated by 28 nucleotides Atu1606 and Atu1607 are separated by 13 nucleotides Atu1607 and Atu1608 are separated by 137 nucleotides
Atu1604: Atu1604 - poly-beta-hydroxybutyrate synthase, at 1,585,294 to 1,587,153
Atu1604
Atu1605: Atu1605 - sugar fermentation stimulation protein, at 1,587,185 to 1,587,904
Atu1605
Atu1606: Atu1606 - methionine aminopeptidase, at 1,587,933 to 1,588,769
Atu1606
Atu1607: Atu1607 - DNA repair protein, at 1,588,783 to 1,589,625
Atu1607
Atu1608: Atu1608 - hypothetical protein, at 1,589,763 to 1,590,185
Atu1608
Position (kb)
1587
1588
1589 Strain fitness (log2 ratio)
-1
0
1 at 1586.978 kb on - strand at 1586.996 kb on - strand at 1587.026 kb on - strand at 1587.092 kb on + strand at 1587.125 kb on - strand at 1587.137 kb on + strand at 1587.399 kb on - strand, within Atu1605 at 1587.474 kb on + strand, within Atu1605 at 1587.512 kb on + strand, within Atu1605 at 1587.550 kb on + strand, within Atu1605 at 1587.692 kb on + strand, within Atu1605 at 1587.704 kb on + strand, within Atu1605 at 1587.714 kb on + strand, within Atu1605 at 1587.714 kb on + strand, within Atu1605 at 1587.714 kb on + strand, within Atu1605 at 1587.722 kb on - strand, within Atu1605 at 1587.722 kb on - strand, within Atu1605 at 1587.722 kb on - strand, within Atu1605 at 1587.769 kb on + strand, within Atu1605 at 1587.777 kb on - strand, within Atu1605 at 1587.807 kb on + strand, within Atu1605 at 1587.815 kb on - strand, within Atu1605 at 1587.822 kb on - strand, within Atu1605 at 1587.957 kb on + strand at 1587.961 kb on + strand at 1587.995 kb on + strand at 1588.003 kb on - strand at 1588.165 kb on - strand, within Atu1606 at 1588.180 kb on - strand, within Atu1606 at 1588.283 kb on - strand, within Atu1606 at 1588.306 kb on + strand, within Atu1606 at 1588.333 kb on - strand, within Atu1606 at 1588.439 kb on - strand, within Atu1606 at 1588.546 kb on - strand, within Atu1606 at 1588.648 kb on + strand, within Atu1606 at 1588.798 kb on + strand at 1588.806 kb on - strand at 1588.806 kb on - strand at 1588.825 kb on + strand at 1588.832 kb on + strand at 1588.857 kb on + strand at 1588.994 kb on - strand, within Atu1607 at 1589.124 kb on - strand, within Atu1607 at 1589.200 kb on - strand, within Atu1607 at 1589.210 kb on + strand, within Atu1607 at 1589.314 kb on + strand, within Atu1607 at 1589.427 kb on - strand, within Atu1607 at 1589.552 kb on + strand at 1589.558 kb on - strand at 1589.758 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nalidixic 0.02 mM remove 1,586,978 - +0.2 1,586,996 - +0.0 1,587,026 - -0.8 1,587,092 + +0.3 1,587,125 - +0.1 1,587,137 + +0.0 1,587,399 - Atu1605 0.30 -0.0 1,587,474 + Atu1605 0.40 -0.8 1,587,512 + Atu1605 0.45 +0.3 1,587,550 + Atu1605 0.51 +0.1 1,587,692 + Atu1605 0.70 -0.0 1,587,704 + Atu1605 0.72 -0.0 1,587,714 + Atu1605 0.73 +0.3 1,587,714 + Atu1605 0.73 -0.1 1,587,714 + Atu1605 0.73 +0.3 1,587,722 - Atu1605 0.75 +0.0 1,587,722 - Atu1605 0.75 +0.1 1,587,722 - Atu1605 0.75 -0.1 1,587,769 + Atu1605 0.81 +0.3 1,587,777 - Atu1605 0.82 -0.0 1,587,807 + Atu1605 0.86 -0.1 1,587,815 - Atu1605 0.88 +0.3 1,587,822 - Atu1605 0.88 -0.1 1,587,957 + -0.6 1,587,961 + +0.4 1,587,995 + -0.3 1,588,003 - -0.7 1,588,165 - Atu1606 0.28 -1.2 1,588,180 - Atu1606 0.30 -0.6 1,588,283 - Atu1606 0.42 -0.7 1,588,306 + Atu1606 0.45 -0.6 1,588,333 - Atu1606 0.48 -1.0 1,588,439 - Atu1606 0.60 -0.1 1,588,546 - Atu1606 0.73 -0.4 1,588,648 + Atu1606 0.85 -0.6 1,588,798 + +0.3 1,588,806 - +0.1 1,588,806 - +0.1 1,588,825 + -0.3 1,588,832 + -0.1 1,588,857 + +0.6 1,588,994 - Atu1607 0.25 +0.5 1,589,124 - Atu1607 0.40 +0.7 1,589,200 - Atu1607 0.49 +0.1 1,589,210 + Atu1607 0.51 -0.4 1,589,314 + Atu1607 0.63 -0.0 1,589,427 - Atu1607 0.76 +1.3 1,589,552 + +0.2 1,589,558 - +0.9 1,589,758 + -0.0
Or see this region's nucleotide sequence