Experiment: R2A with Spectinomycin 0.002 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0725 and Atu0726 are separated by 328 nucleotides Atu0726 and Atu0727 are separated by 157 nucleotides
Atu0725: Atu0725 - hypothetical protein, at 725,233 to 725,844
Atu0725
Atu0726: Atu0726 - ring hydroxylating dioxygenase, alpha-subunit, at 726,173 to 727,417
Atu0726
Atu0727: Atu0727 - ferredoxin I, at 727,575 to 728,507
Atu0727
Position (kb)
726
727
728 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 725.174 kb on - strand at 725.259 kb on + strand at 725.372 kb on - strand, within Atu0725 at 725.615 kb on - strand, within Atu0725 at 725.617 kb on + strand, within Atu0725 at 725.703 kb on + strand, within Atu0725 at 725.724 kb on + strand, within Atu0725 at 725.724 kb on + strand, within Atu0725 at 725.779 kb on - strand, within Atu0725 at 725.779 kb on - strand, within Atu0725 at 725.779 kb on - strand, within Atu0725 at 725.779 kb on - strand, within Atu0725 at 725.800 kb on - strand at 725.805 kb on - strand at 725.847 kb on - strand at 725.877 kb on + strand at 725.878 kb on + strand at 725.896 kb on - strand at 725.903 kb on + strand at 725.927 kb on + strand at 725.929 kb on + strand at 725.937 kb on - strand at 726.083 kb on + strand at 726.120 kb on - strand at 726.191 kb on + strand at 726.236 kb on - strand at 726.249 kb on - strand at 726.264 kb on - strand at 726.343 kb on + strand, within Atu0726 at 726.351 kb on - strand, within Atu0726 at 726.363 kb on + strand, within Atu0726 at 726.371 kb on - strand, within Atu0726 at 726.481 kb on + strand, within Atu0726 at 726.512 kb on - strand, within Atu0726 at 726.536 kb on + strand, within Atu0726 at 726.538 kb on + strand, within Atu0726 at 726.538 kb on + strand, within Atu0726 at 726.665 kb on + strand, within Atu0726 at 726.700 kb on + strand, within Atu0726 at 726.744 kb on - strand, within Atu0726 at 726.803 kb on - strand, within Atu0726 at 726.816 kb on + strand, within Atu0726 at 726.881 kb on - strand, within Atu0726 at 726.948 kb on - strand, within Atu0726 at 727.021 kb on + strand, within Atu0726 at 727.105 kb on + strand, within Atu0726 at 727.134 kb on + strand, within Atu0726 at 727.179 kb on - strand, within Atu0726 at 727.300 kb on + strand at 727.305 kb on + strand at 727.378 kb on + strand at 727.393 kb on + strand at 727.394 kb on + strand at 727.399 kb on + strand at 727.459 kb on + strand at 727.554 kb on + strand at 727.557 kb on - strand at 727.631 kb on + strand at 727.631 kb on + strand at 727.633 kb on + strand at 727.633 kb on + strand at 727.633 kb on + strand at 727.633 kb on + strand at 727.641 kb on - strand at 727.641 kb on - strand at 727.641 kb on - strand at 727.645 kb on + strand at 727.651 kb on - strand at 727.653 kb on - strand at 727.653 kb on - strand at 727.736 kb on - strand, within Atu0727 at 727.741 kb on + strand, within Atu0727 at 727.886 kb on - strand, within Atu0727 at 727.946 kb on + strand, within Atu0727 at 727.966 kb on + strand, within Atu0727 at 728.020 kb on + strand, within Atu0727 at 728.033 kb on - strand, within Atu0727 at 728.033 kb on - strand, within Atu0727 at 728.048 kb on + strand, within Atu0727 at 728.056 kb on - strand, within Atu0727 at 728.104 kb on + strand, within Atu0727 at 728.112 kb on - strand, within Atu0727 at 728.244 kb on + strand, within Atu0727 at 728.252 kb on - strand, within Atu0727 at 728.270 kb on + strand, within Atu0727 at 728.307 kb on + strand, within Atu0727 at 728.315 kb on - strand, within Atu0727 at 728.315 kb on - strand, within Atu0727
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.002 mM remove 725,174 - +0.2 725,259 + +0.1 725,372 - Atu0725 0.23 -0.2 725,615 - Atu0725 0.62 +0.9 725,617 + Atu0725 0.63 +0.7 725,703 + Atu0725 0.77 -0.1 725,724 + Atu0725 0.80 -0.5 725,724 + Atu0725 0.80 +0.1 725,779 - Atu0725 0.89 -0.1 725,779 - Atu0725 0.89 -0.5 725,779 - Atu0725 0.89 +0.1 725,779 - Atu0725 0.89 +0.6 725,800 - +0.3 725,805 - +0.4 725,847 - -0.7 725,877 + -0.1 725,878 + -0.5 725,896 - +0.2 725,903 + +0.5 725,927 + +0.4 725,929 + +0.5 725,937 - -0.0 726,083 + -0.1 726,120 - -0.3 726,191 + -0.5 726,236 - -1.8 726,249 - -0.1 726,264 - +0.2 726,343 + Atu0726 0.14 -0.9 726,351 - Atu0726 0.14 -0.7 726,363 + Atu0726 0.15 +0.7 726,371 - Atu0726 0.16 -0.7 726,481 + Atu0726 0.25 +0.4 726,512 - Atu0726 0.27 -0.3 726,536 + Atu0726 0.29 -1.2 726,538 + Atu0726 0.29 -0.7 726,538 + Atu0726 0.29 -0.5 726,665 + Atu0726 0.40 -1.1 726,700 + Atu0726 0.42 +0.5 726,744 - Atu0726 0.46 -0.6 726,803 - Atu0726 0.51 -1.0 726,816 + Atu0726 0.52 -1.2 726,881 - Atu0726 0.57 -0.9 726,948 - Atu0726 0.62 -1.0 727,021 + Atu0726 0.68 -0.9 727,105 + Atu0726 0.75 -0.6 727,134 + Atu0726 0.77 -0.5 727,179 - Atu0726 0.81 -0.5 727,300 + -0.4 727,305 + -1.1 727,378 + -0.0 727,393 + +1.8 727,394 + -0.8 727,399 + +0.1 727,459 + -1.2 727,554 + -0.3 727,557 - -0.1 727,631 + -0.6 727,631 + +0.1 727,633 + -0.7 727,633 + +0.6 727,633 + -0.6 727,633 + +0.5 727,641 - -1.0 727,641 - -0.6 727,641 - -0.1 727,645 + -0.4 727,651 - -1.1 727,653 - -0.2 727,653 - -0.3 727,736 - Atu0727 0.17 -0.7 727,741 + Atu0727 0.18 -0.9 727,886 - Atu0727 0.33 -0.8 727,946 + Atu0727 0.40 -0.9 727,966 + Atu0727 0.42 -0.9 728,020 + Atu0727 0.48 +0.2 728,033 - Atu0727 0.49 +0.2 728,033 - Atu0727 0.49 -0.1 728,048 + Atu0727 0.51 -0.6 728,056 - Atu0727 0.52 -0.8 728,104 + Atu0727 0.57 -0.4 728,112 - Atu0727 0.58 -1.0 728,244 + Atu0727 0.72 -0.1 728,252 - Atu0727 0.73 -1.0 728,270 + Atu0727 0.74 -0.5 728,307 + Atu0727 0.78 -0.9 728,315 - Atu0727 0.79 -0.8 728,315 - Atu0727 0.79 -0.8
Or see this region's nucleotide sequence