Experiment: R2A with Tetracycline 0.002 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2580 and Atu8180 are separated by 37 nucleotides Atu8180 and Atu2582 are separated by 4 nucleotides
Atu2580: Atu2580 - adenylate cyclase, at 2,554,576 to 2,556,270
Atu2580
Atu8180: Atu8180 - hypothetical protein, at 2,556,308 to 2,556,790
Atu8180
Atu2582: Atu2582 - UDP-glucose/GDP-mannose dehydrogenase, at 2,556,795 to 2,558,069
Atu2582
Position (kb)
2556
2557 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2555.369 kb on + strand, within Atu2580 at 2555.369 kb on + strand, within Atu2580 at 2555.377 kb on - strand, within Atu2580 at 2555.505 kb on - strand, within Atu2580 at 2555.505 kb on - strand, within Atu2580 at 2555.596 kb on - strand, within Atu2580 at 2555.607 kb on - strand, within Atu2580 at 2555.618 kb on + strand, within Atu2580 at 2555.742 kb on + strand, within Atu2580 at 2555.757 kb on + strand, within Atu2580 at 2555.776 kb on + strand, within Atu2580 at 2555.820 kb on + strand, within Atu2580 at 2555.820 kb on + strand, within Atu2580 at 2555.867 kb on + strand, within Atu2580 at 2555.898 kb on - strand, within Atu2580 at 2556.153 kb on + strand at 2556.161 kb on - strand at 2556.163 kb on + strand at 2556.168 kb on + strand at 2556.171 kb on - strand at 2556.171 kb on - strand at 2556.171 kb on - strand at 2556.227 kb on + strand at 2556.245 kb on - strand at 2556.455 kb on + strand, within Atu8180 at 2556.548 kb on - strand, within Atu8180 at 2556.600 kb on + strand, within Atu8180 at 2556.608 kb on - strand, within Atu8180 at 2556.608 kb on - strand, within Atu8180 at 2556.711 kb on - strand, within Atu8180 at 2556.720 kb on - strand, within Atu8180 at 2556.734 kb on + strand, within Atu8180 at 2556.734 kb on + strand, within Atu8180 at 2556.741 kb on + strand, within Atu8180 at 2556.741 kb on - strand, within Atu8180 at 2556.749 kb on - strand at 2556.784 kb on - strand at 2556.898 kb on - strand at 2556.959 kb on - strand, within Atu2582 at 2557.030 kb on - strand, within Atu2582 at 2557.154 kb on - strand, within Atu2582 at 2557.319 kb on + strand, within Atu2582 at 2557.363 kb on - strand, within Atu2582 at 2557.422 kb on - strand, within Atu2582 at 2557.573 kb on + strand, within Atu2582 at 2557.678 kb on - strand, within Atu2582 at 2557.680 kb on + strand, within Atu2582 at 2557.682 kb on + strand, within Atu2582 at 2557.698 kb on - strand, within Atu2582 at 2557.725 kb on - strand, within Atu2582 at 2557.758 kb on - strand, within Atu2582 at 2557.760 kb on + strand, within Atu2582 at 2557.760 kb on + strand, within Atu2582
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Tetracycline 0.002 mM remove 2,555,369 + Atu2580 0.47 -1.6 2,555,369 + Atu2580 0.47 -0.4 2,555,377 - Atu2580 0.47 +0.7 2,555,505 - Atu2580 0.55 +0.7 2,555,505 - Atu2580 0.55 +0.1 2,555,596 - Atu2580 0.60 +0.0 2,555,607 - Atu2580 0.61 -0.1 2,555,618 + Atu2580 0.61 +0.0 2,555,742 + Atu2580 0.69 -1.1 2,555,757 + Atu2580 0.70 -0.1 2,555,776 + Atu2580 0.71 -2.1 2,555,820 + Atu2580 0.73 +0.6 2,555,820 + Atu2580 0.73 +0.8 2,555,867 + Atu2580 0.76 -0.1 2,555,898 - Atu2580 0.78 -0.1 2,556,153 + +1.3 2,556,161 - -0.2 2,556,163 + -0.2 2,556,168 + +0.0 2,556,171 - +2.4 2,556,171 - +0.7 2,556,171 - +0.5 2,556,227 + +1.7 2,556,245 - +1.0 2,556,455 + Atu8180 0.30 -2.7 2,556,548 - Atu8180 0.50 +0.8 2,556,600 + Atu8180 0.60 -2.2 2,556,608 - Atu8180 0.62 -2.5 2,556,608 - Atu8180 0.62 -0.5 2,556,711 - Atu8180 0.83 -1.9 2,556,720 - Atu8180 0.85 -0.4 2,556,734 + Atu8180 0.88 -1.4 2,556,734 + Atu8180 0.88 -1.4 2,556,741 + Atu8180 0.90 +0.5 2,556,741 - Atu8180 0.90 -1.9 2,556,749 - -1.8 2,556,784 - +1.0 2,556,898 - -1.8 2,556,959 - Atu2582 0.13 -1.9 2,557,030 - Atu2582 0.18 -0.8 2,557,154 - Atu2582 0.28 +2.2 2,557,319 + Atu2582 0.41 -1.8 2,557,363 - Atu2582 0.45 +0.4 2,557,422 - Atu2582 0.49 -0.7 2,557,573 + Atu2582 0.61 -2.8 2,557,678 - Atu2582 0.69 -0.7 2,557,680 + Atu2582 0.69 +0.0 2,557,682 + Atu2582 0.70 +1.0 2,557,698 - Atu2582 0.71 -2.4 2,557,725 - Atu2582 0.73 -0.5 2,557,758 - Atu2582 0.76 +0.1 2,557,760 + Atu2582 0.76 +0.2 2,557,760 + Atu2582 0.76 +0.5
Or see this region's nucleotide sequence