Experiment: R2A with Tetracycline 0.002 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3519 and Atu3520 are separated by 242 nucleotides Atu3520 and Atu3521 are separated by 147 nucleotides
Atu3519: Atu3519 - peptidyl-prolyl cis-trans isomerase, at 566,148 to 567,014
Atu3519
Atu3520: Atu3520 - preprotein translocase subunit SecA, at 567,257 to 569,965
Atu3520
Atu3521: Atu3521 - permease, at 570,113 to 571,006
Atu3521
Position (kb) 567
568
569
570 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 566.294 kb on - strand, within Atu3519 at 566.365 kb on - strand, within Atu3519 at 566.394 kb on - strand, within Atu3519 at 566.412 kb on + strand, within Atu3519 at 566.471 kb on - strand, within Atu3519 at 566.471 kb on - strand, within Atu3519 at 566.567 kb on + strand, within Atu3519 at 566.619 kb on - strand, within Atu3519 at 566.654 kb on - strand, within Atu3519 at 566.654 kb on - strand, within Atu3519 at 566.698 kb on + strand, within Atu3519 at 566.811 kb on + strand, within Atu3519 at 566.811 kb on + strand, within Atu3519 at 566.811 kb on + strand, within Atu3519 at 566.819 kb on - strand, within Atu3519 at 566.819 kb on - strand, within Atu3519 at 566.824 kb on + strand, within Atu3519 at 566.866 kb on + strand, within Atu3519 at 566.957 kb on + strand at 567.013 kb on + strand at 570.003 kb on - strand at 570.052 kb on + strand at 570.083 kb on - strand at 570.243 kb on + strand, within Atu3521 at 570.442 kb on + strand, within Atu3521 at 570.442 kb on + strand, within Atu3521 at 570.475 kb on - strand, within Atu3521 at 570.604 kb on - strand, within Atu3521 at 570.633 kb on - strand, within Atu3521 at 570.731 kb on - strand, within Atu3521 at 570.795 kb on + strand, within Atu3521 at 570.837 kb on + strand, within Atu3521 at 570.931 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Tetracycline 0.002 mM remove 566,294 - Atu3519 0.17 +0.2 566,365 - Atu3519 0.25 +1.2 566,394 - Atu3519 0.28 +0.4 566,412 + Atu3519 0.30 +1.4 566,471 - Atu3519 0.37 +0.5 566,471 - Atu3519 0.37 +1.2 566,567 + Atu3519 0.48 +1.1 566,619 - Atu3519 0.54 +0.6 566,654 - Atu3519 0.58 +0.3 566,654 - Atu3519 0.58 +1.5 566,698 + Atu3519 0.63 +3.2 566,811 + Atu3519 0.76 +1.3 566,811 + Atu3519 0.76 +2.1 566,811 + Atu3519 0.76 +0.8 566,819 - Atu3519 0.77 +0.9 566,819 - Atu3519 0.77 +1.4 566,824 + Atu3519 0.78 +0.9 566,866 + Atu3519 0.83 +0.6 566,957 + +1.2 567,013 + -0.9 570,003 - -1.1 570,052 + +1.6 570,083 - -0.1 570,243 + Atu3521 0.15 +0.0 570,442 + Atu3521 0.37 +0.3 570,442 + Atu3521 0.37 +0.2 570,475 - Atu3521 0.40 +0.7 570,604 - Atu3521 0.55 +0.4 570,633 - Atu3521 0.58 -0.6 570,731 - Atu3521 0.69 +1.9 570,795 + Atu3521 0.76 -2.1 570,837 + Atu3521 0.81 -0.1 570,931 + +0.7
Or see this region's nucleotide sequence