Strain Fitness in Agrobacterium fabrum C58 around Atu2548

Experiment: R2A with Tetracycline 0.002 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAtu2547 and Atu2548 are separated by 586 nucleotidesAtu2548 and Atu2549 are separated by 3 nucleotidesAtu2549 and Atu2550 are separated by 199 nucleotides Atu2547: Atu2547 - 16S ribosomal RNA, at 2,517,145 to 2,518,624 Atu2547 Atu2548: Atu2548 - phosphoribosylaminoimidazole-succinocarboxamide synthase, at 2,519,211 to 2,520,161 Atu2548 Atu2549: Atu2549 - transcriptional regulator, TetR family, at 2,520,165 to 2,520,824 Atu2549 Atu2550: Atu2550 - RND multidrug efflux membrane permease, at 2,521,024 to 2,522,226 Atu2550 Position (kb) 2519 2520 2521Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 2519.116 kb on + strandat 2519.124 kb on - strandat 2519.124 kb on - strandat 2519.197 kb on + strandat 2519.246 kb on + strandat 2519.278 kb on + strandat 2519.286 kb on - strandat 2519.286 kb on - strandat 2519.288 kb on + strandat 2519.332 kb on + strand, within Atu2548at 2519.337 kb on + strand, within Atu2548at 2519.368 kb on + strand, within Atu2548at 2519.401 kb on - strand, within Atu2548at 2519.406 kb on - strand, within Atu2548at 2519.408 kb on + strand, within Atu2548at 2519.430 kb on + strand, within Atu2548at 2519.486 kb on - strand, within Atu2548at 2519.494 kb on + strand, within Atu2548at 2519.525 kb on + strand, within Atu2548at 2519.533 kb on + strand, within Atu2548at 2519.547 kb on + strand, within Atu2548at 2519.549 kb on + strand, within Atu2548at 2519.559 kb on + strand, within Atu2548at 2519.565 kb on + strand, within Atu2548at 2519.570 kb on + strand, within Atu2548at 2519.578 kb on - strand, within Atu2548at 2519.608 kb on + strand, within Atu2548at 2519.681 kb on - strand, within Atu2548at 2519.692 kb on - strand, within Atu2548at 2519.725 kb on + strand, within Atu2548at 2519.733 kb on - strand, within Atu2548at 2519.735 kb on + strand, within Atu2548at 2519.748 kb on - strand, within Atu2548at 2519.756 kb on + strand, within Atu2548at 2519.756 kb on + strand, within Atu2548at 2519.756 kb on + strand, within Atu2548at 2519.756 kb on + strand, within Atu2548at 2519.764 kb on - strand, within Atu2548at 2519.764 kb on - strand, within Atu2548at 2519.764 kb on - strand, within Atu2548at 2519.774 kb on - strand, within Atu2548at 2519.799 kb on - strand, within Atu2548at 2519.799 kb on - strand, within Atu2548at 2519.877 kb on - strand, within Atu2548at 2519.963 kb on + strand, within Atu2548at 2519.990 kb on + strand, within Atu2548at 2520.038 kb on - strand, within Atu2548at 2520.044 kb on + strand, within Atu2548at 2520.044 kb on + strand, within Atu2548at 2520.044 kb on + strand, within Atu2548at 2520.052 kb on - strand, within Atu2548at 2520.052 kb on - strand, within Atu2548at 2520.098 kb on - strandat 2520.133 kb on + strandat 2520.133 kb on + strandat 2520.155 kb on + strandat 2520.155 kb on + strandat 2520.155 kb on + strandat 2520.163 kb on - strandat 2520.344 kb on - strand, within Atu2549at 2520.347 kb on - strand, within Atu2549at 2520.349 kb on - strand, within Atu2549at 2520.434 kb on + strand, within Atu2549at 2520.457 kb on + strand, within Atu2549at 2520.475 kb on + strand, within Atu2549at 2520.504 kb on + strand, within Atu2549at 2520.512 kb on - strand, within Atu2549at 2520.554 kb on - strand, within Atu2549at 2520.560 kb on - strand, within Atu2549at 2520.562 kb on + strand, within Atu2549at 2520.593 kb on + strand, within Atu2549at 2520.635 kb on + strand, within Atu2549at 2520.640 kb on + strand, within Atu2549at 2520.651 kb on + strand, within Atu2549at 2520.707 kb on + strand, within Atu2549at 2520.710 kb on - strand, within Atu2549at 2520.711 kb on + strand, within Atu2549at 2520.806 kb on - strandat 2520.866 kb on + strandat 2520.866 kb on + strandat 2520.874 kb on - strandat 2520.874 kb on - strandat 2520.881 kb on - strandat 2520.897 kb on + strandat 2520.926 kb on + strandat 2520.942 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Tetracycline 0.002 mM
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2,519,116 + +2.1
2,519,124 - +2.0
2,519,124 - +2.4
2,519,197 + +0.3
2,519,246 + +2.8
2,519,278 + +2.5
2,519,286 - +1.7
2,519,286 - +2.4
2,519,288 + +1.9
2,519,332 + Atu2548 0.13 +1.8
2,519,337 + Atu2548 0.13 +1.4
2,519,368 + Atu2548 0.17 +1.9
2,519,401 - Atu2548 0.20 +1.3
2,519,406 - Atu2548 0.21 +0.9
2,519,408 + Atu2548 0.21 +1.5
2,519,430 + Atu2548 0.23 +1.8
2,519,486 - Atu2548 0.29 +2.9
2,519,494 + Atu2548 0.30 +1.3
2,519,525 + Atu2548 0.33 +2.2
2,519,533 + Atu2548 0.34 +1.9
2,519,547 + Atu2548 0.35 +1.7
2,519,549 + Atu2548 0.36 +1.3
2,519,559 + Atu2548 0.37 +2.6
2,519,565 + Atu2548 0.37 +0.7
2,519,570 + Atu2548 0.38 -0.1
2,519,578 - Atu2548 0.39 +1.6
2,519,608 + Atu2548 0.42 +2.4
2,519,681 - Atu2548 0.49 +1.4
2,519,692 - Atu2548 0.51 +2.1
2,519,725 + Atu2548 0.54 +3.0
2,519,733 - Atu2548 0.55 -0.1
2,519,735 + Atu2548 0.55 +1.3
2,519,748 - Atu2548 0.56 +1.5
2,519,756 + Atu2548 0.57 +3.0
2,519,756 + Atu2548 0.57 +1.5
2,519,756 + Atu2548 0.57 +2.7
2,519,756 + Atu2548 0.57 +1.1
2,519,764 - Atu2548 0.58 +2.1
2,519,764 - Atu2548 0.58 +1.7
2,519,764 - Atu2548 0.58 +1.5
2,519,774 - Atu2548 0.59 +1.9
2,519,799 - Atu2548 0.62 +1.3
2,519,799 - Atu2548 0.62 +1.1
2,519,877 - Atu2548 0.70 +2.3
2,519,963 + Atu2548 0.79 -0.2
2,519,990 + Atu2548 0.82 +1.9
2,520,038 - Atu2548 0.87 +1.3
2,520,044 + Atu2548 0.88 +1.0
2,520,044 + Atu2548 0.88 -0.3
2,520,044 + Atu2548 0.88 +0.8
2,520,052 - Atu2548 0.88 +0.7
2,520,052 - Atu2548 0.88 +1.4
2,520,098 - +1.3
2,520,133 + +1.8
2,520,133 + +0.2
2,520,155 + -1.0
2,520,155 + +0.8
2,520,155 + -0.5
2,520,163 - +2.6
2,520,344 - Atu2549 0.27 +1.7
2,520,347 - Atu2549 0.28 +2.5
2,520,349 - Atu2549 0.28 +3.4
2,520,434 + Atu2549 0.41 +3.4
2,520,457 + Atu2549 0.44 +2.9
2,520,475 + Atu2549 0.47 +3.4
2,520,504 + Atu2549 0.51 +3.2
2,520,512 - Atu2549 0.53 +3.3
2,520,554 - Atu2549 0.59 +3.2
2,520,560 - Atu2549 0.60 +3.5
2,520,562 + Atu2549 0.60 +3.2
2,520,593 + Atu2549 0.65 +3.2
2,520,635 + Atu2549 0.71 +3.1
2,520,640 + Atu2549 0.72 +2.8
2,520,651 + Atu2549 0.74 +3.4
2,520,707 + Atu2549 0.82 +2.2
2,520,710 - Atu2549 0.83 +4.0
2,520,711 + Atu2549 0.83 +3.6
2,520,806 - -0.6
2,520,866 + -2.5
2,520,866 + -1.5
2,520,874 - -1.8
2,520,874 - +2.2
2,520,881 - -2.4
2,520,897 + +0.6
2,520,926 + -1.7
2,520,942 - -1.6

Or see this region's nucleotide sequence