Experiment: R2A with Tetracycline 0.002 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0382 and Atu0383 are separated by 12 nucleotides Atu0383 and Atu0384 overlap by 4 nucleotides Atu0384 and Atu0385 overlap by 1 nucleotides Atu0385 and Atu8066 are separated by 36 nucleotides Atu8066 and Atu0386 are separated by 41 nucleotides Atu0386 and Atu0387 are separated by 37 nucleotides
Atu0382: Atu0382 - hypothetical protein, at 375,023 to 375,400
Atu0382
Atu0383: Atu0383 - GTP-binding protein, at 375,413 to 376,066
Atu0383
Atu0384: Atu0384 - preprotein translocase 60 kD membrane subunit, at 376,063 to 377,862
Atu0384
Atu0385: Atu0385 - ribonuclease P protein component, at 377,862 to 378,245
Atu0385
Atu8066: Atu8066 - 50S ribosomal protein L34, at 378,282 to 378,419
Atu8066
Atu0386: Atu0386 - hypothetical protein, at 378,461 to 378,733
Atu0386
Atu0387: Atu0387 - methyl-accepting chemotaxis protein, at 378,771 to 380,819
Atu0387
Position (kb) 376
377
378 Strain fitness (log2 ratio) -1
0
1 at 375.238 kb on + strand, within Atu0382 at 375.249 kb on + strand, within Atu0382 at 375.252 kb on - strand, within Atu0382 at 375.257 kb on - strand, within Atu0382 at 375.372 kb on - strand at 375.382 kb on + strand at 375.382 kb on + strand at 378.702 kb on - strand, within Atu0386 at 378.814 kb on + strand at 378.824 kb on + strand at 378.832 kb on + strand at 378.840 kb on + strand at 378.842 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Tetracycline 0.002 mM remove 375,238 + Atu0382 0.57 -1.7 375,249 + Atu0382 0.60 -1.2 375,252 - Atu0382 0.61 -0.7 375,257 - Atu0382 0.62 -0.8 375,372 - -0.2 375,382 + -0.0 375,382 + -0.0 378,702 - Atu0386 0.88 +0.6 378,814 + -0.5 378,824 + -1.4 378,832 + +0.4 378,840 + +0.2 378,842 + +0.9
Or see this region's nucleotide sequence