Experiment: R2A with Kanamycin sulfate 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0482 and Atu0483 are separated by 61 nucleotides Atu0483 and Atu0484 are separated by 112 nucleotides Atu0484 and Atu0485 are separated by 252 nucleotides Atu0485 and Atu0486 overlap by 1 nucleotides
Atu0482: Atu0482 - polysaccharide deacetylase, at 471,433 to 472,185
Atu0482
Atu0483: Atu0483 - bacteriophage repressor protein C1, at 472,247 to 472,897
Atu0483
Atu0484: Atu0484 - two component response regulator, at 473,010 to 473,660
Atu0484
Atu0485: Atu0485 - hypothetical protein, at 473,913 to 474,266
Atu0485
Atu0486: Atu0486 - dihydroorotate dehydrogenase, at 474,266 to 475,357
Atu0486
Position (kb) 473
474 Strain fitness (log2 ratio) -2
-1
0
1 at 472.052 kb on + strand, within Atu0482 at 472.143 kb on + strand at 472.254 kb on - strand at 472.254 kb on - strand at 473.062 kb on - strand at 473.074 kb on + strand at 473.075 kb on + strand, within Atu0484 at 473.080 kb on + strand, within Atu0484 at 473.128 kb on + strand, within Atu0484 at 473.237 kb on + strand, within Atu0484 at 473.237 kb on + strand, within Atu0484 at 473.237 kb on + strand, within Atu0484 at 473.252 kb on + strand, within Atu0484 at 473.292 kb on + strand, within Atu0484 at 473.292 kb on + strand, within Atu0484 at 473.313 kb on + strand, within Atu0484 at 473.379 kb on + strand, within Atu0484 at 473.399 kb on - strand, within Atu0484 at 473.568 kb on + strand, within Atu0484 at 473.598 kb on - strand at 473.783 kb on + strand at 473.898 kb on + strand at 473.898 kb on + strand at 473.906 kb on - strand at 473.969 kb on + strand, within Atu0485 at 473.977 kb on - strand, within Atu0485 at 474.181 kb on + strand, within Atu0485 at 474.203 kb on + strand, within Atu0485 at 474.300 kb on + strand at 474.482 kb on - strand, within Atu0486 at 474.574 kb on + strand, within Atu0486 at 474.632 kb on + strand, within Atu0486
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Kanamycin sulfate 0.02 mM remove 472,052 + Atu0482 0.82 +0.1 472,143 + +0.6 472,254 - +0.4 472,254 - -0.0 473,062 - +0.2 473,074 + -0.1 473,075 + Atu0484 0.10 -0.4 473,080 + Atu0484 0.11 -1.6 473,128 + Atu0484 0.18 -0.7 473,237 + Atu0484 0.35 -0.7 473,237 + Atu0484 0.35 -0.5 473,237 + Atu0484 0.35 +0.2 473,252 + Atu0484 0.37 +0.4 473,292 + Atu0484 0.43 -0.5 473,292 + Atu0484 0.43 +0.2 473,313 + Atu0484 0.47 -0.7 473,379 + Atu0484 0.57 -0.5 473,399 - Atu0484 0.60 -0.3 473,568 + Atu0484 0.86 -0.2 473,598 - +0.7 473,783 + -0.8 473,898 + -0.3 473,898 + +0.3 473,906 - -1.4 473,969 + Atu0485 0.16 -0.1 473,977 - Atu0485 0.18 -0.4 474,181 + Atu0485 0.76 -0.2 474,203 + Atu0485 0.82 -0.1 474,300 + -2.0 474,482 - Atu0486 0.20 -1.4 474,574 + Atu0486 0.28 -1.9 474,632 + Atu0486 0.34 -1.1
Or see this region's nucleotide sequence