Experiment: R2A with rubidium chloride 600 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5047 and Atu5048 are separated by 84 nucleotides Atu5048 and Atu8099 are separated by 11 nucleotides Atu8099 and Atu5049 are separated by 199 nucleotides
Atu5047: Atu5047 - hypothetical protein, at 46,905 to 47,141
Atu5047
Atu5048: Atu5048 - exodeoxyribonuclease III, at 47,226 to 48,002
Atu5048
Atu8099: Atu8099 - hypothetical protein, at 48,014 to 48,190
Atu8099
Atu5049: Atu5049 - hypothetical protein, at 48,390 to 49,286
Atu5049
Position (kb)
48
49 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 47.027 kb on + strand, within Atu5047 at 47.059 kb on + strand, within Atu5047 at 47.125 kb on - strand at 47.157 kb on - strand at 47.157 kb on - strand at 47.170 kb on - strand at 47.188 kb on + strand at 47.240 kb on + strand at 47.240 kb on + strand at 47.245 kb on + strand at 47.253 kb on - strand at 47.253 kb on - strand at 47.275 kb on + strand at 47.283 kb on - strand at 47.307 kb on + strand, within Atu5048 at 47.307 kb on + strand, within Atu5048 at 47.315 kb on - strand, within Atu5048 at 47.315 kb on - strand, within Atu5048 at 47.327 kb on + strand, within Atu5048 at 47.327 kb on + strand, within Atu5048 at 47.327 kb on - strand, within Atu5048 at 47.335 kb on - strand, within Atu5048 at 47.417 kb on + strand, within Atu5048 at 47.446 kb on + strand, within Atu5048 at 47.455 kb on + strand, within Atu5048 at 47.459 kb on - strand, within Atu5048 at 47.469 kb on + strand, within Atu5048 at 47.477 kb on - strand, within Atu5048 at 47.477 kb on - strand, within Atu5048 at 47.491 kb on + strand, within Atu5048 at 47.491 kb on + strand, within Atu5048 at 47.494 kb on - strand, within Atu5048 at 47.598 kb on - strand, within Atu5048 at 47.600 kb on + strand, within Atu5048 at 47.608 kb on - strand, within Atu5048 at 47.622 kb on - strand, within Atu5048 at 47.622 kb on - strand, within Atu5048 at 47.622 kb on - strand, within Atu5048 at 47.622 kb on - strand, within Atu5048 at 47.652 kb on + strand, within Atu5048 at 47.786 kb on + strand, within Atu5048 at 47.925 kb on - strand at 47.954 kb on - strand at 47.973 kb on - strand at 48.017 kb on + strand at 48.038 kb on + strand, within Atu8099 at 48.103 kb on + strand, within Atu8099 at 48.126 kb on + strand, within Atu8099 at 48.144 kb on + strand, within Atu8099 at 48.188 kb on - strand at 48.193 kb on - strand at 48.198 kb on - strand at 48.291 kb on - strand at 48.385 kb on + strand at 48.458 kb on + strand at 48.461 kb on - strand at 48.461 kb on - strand at 48.463 kb on - strand at 48.466 kb on - strand at 48.466 kb on - strand at 48.466 kb on - strand at 48.466 kb on - strand at 48.466 kb on - strand at 48.506 kb on - strand, within Atu5049 at 48.581 kb on + strand, within Atu5049 at 48.581 kb on + strand, within Atu5049 at 48.581 kb on + strand, within Atu5049 at 48.581 kb on + strand, within Atu5049 at 48.581 kb on + strand, within Atu5049 at 48.589 kb on - strand, within Atu5049 at 48.589 kb on - strand, within Atu5049 at 48.589 kb on - strand, within Atu5049 at 48.648 kb on + strand, within Atu5049 at 48.656 kb on - strand, within Atu5049 at 48.722 kb on - strand, within Atu5049 at 48.733 kb on + strand, within Atu5049 at 48.760 kb on - strand, within Atu5049 at 48.795 kb on + strand, within Atu5049 at 48.815 kb on - strand, within Atu5049 at 48.830 kb on - strand, within Atu5049 at 48.846 kb on + strand, within Atu5049 at 48.863 kb on + strand, within Atu5049 at 48.921 kb on - strand, within Atu5049 at 48.937 kb on + strand, within Atu5049 at 48.991 kb on + strand, within Atu5049 at 49.064 kb on + strand, within Atu5049 at 49.079 kb on - strand, within Atu5049 at 49.161 kb on - strand, within Atu5049
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with rubidium chloride 600 mM remove 47,027 + Atu5047 0.51 +0.6 47,059 + Atu5047 0.65 -0.1 47,125 - +0.5 47,157 - -0.0 47,157 - +0.2 47,170 - -0.1 47,188 + +0.1 47,240 + -1.1 47,240 + -0.3 47,245 + +0.5 47,253 - -0.7 47,253 - +0.7 47,275 + -0.4 47,283 - -0.2 47,307 + Atu5048 0.10 +1.0 47,307 + Atu5048 0.10 -0.4 47,315 - Atu5048 0.11 -0.4 47,315 - Atu5048 0.11 +0.1 47,327 + Atu5048 0.13 -0.6 47,327 + Atu5048 0.13 +0.6 47,327 - Atu5048 0.13 -0.1 47,335 - Atu5048 0.14 +0.7 47,417 + Atu5048 0.25 -0.1 47,446 + Atu5048 0.28 -0.2 47,455 + Atu5048 0.29 -0.5 47,459 - Atu5048 0.30 +0.6 47,469 + Atu5048 0.31 -0.1 47,477 - Atu5048 0.32 -0.5 47,477 - Atu5048 0.32 -1.0 47,491 + Atu5048 0.34 -0.5 47,491 + Atu5048 0.34 +1.1 47,494 - Atu5048 0.34 +0.1 47,598 - Atu5048 0.48 -2.2 47,600 + Atu5048 0.48 -0.7 47,608 - Atu5048 0.49 -0.6 47,622 - Atu5048 0.51 +0.2 47,622 - Atu5048 0.51 +0.9 47,622 - Atu5048 0.51 +0.3 47,622 - Atu5048 0.51 +0.5 47,652 + Atu5048 0.55 -1.6 47,786 + Atu5048 0.72 +1.1 47,925 - +0.5 47,954 - +1.1 47,973 - +0.3 48,017 + +0.4 48,038 + Atu8099 0.14 -1.0 48,103 + Atu8099 0.50 -1.0 48,126 + Atu8099 0.63 -0.5 48,144 + Atu8099 0.73 -3.7 48,188 - -0.3 48,193 - +0.5 48,198 - -0.9 48,291 - -0.5 48,385 + -0.0 48,458 + +0.1 48,461 - +0.1 48,461 - +0.0 48,463 - +1.1 48,466 - -1.4 48,466 - -0.6 48,466 - +0.5 48,466 - -0.1 48,466 - -0.0 48,506 - Atu5049 0.13 -0.3 48,581 + Atu5049 0.21 -1.2 48,581 + Atu5049 0.21 +0.2 48,581 + Atu5049 0.21 +0.7 48,581 + Atu5049 0.21 +2.3 48,581 + Atu5049 0.21 +0.2 48,589 - Atu5049 0.22 +0.8 48,589 - Atu5049 0.22 +0.5 48,589 - Atu5049 0.22 -0.1 48,648 + Atu5049 0.29 -0.3 48,656 - Atu5049 0.30 -0.3 48,722 - Atu5049 0.37 +0.1 48,733 + Atu5049 0.38 -0.1 48,760 - Atu5049 0.41 -1.1 48,795 + Atu5049 0.45 +0.1 48,815 - Atu5049 0.47 +0.1 48,830 - Atu5049 0.49 -0.3 48,846 + Atu5049 0.51 +0.1 48,863 + Atu5049 0.53 +0.3 48,921 - Atu5049 0.59 +0.5 48,937 + Atu5049 0.61 +0.3 48,991 + Atu5049 0.67 +0.0 49,064 + Atu5049 0.75 +0.8 49,079 - Atu5049 0.77 -1.1 49,161 - Atu5049 0.86 +0.1
Or see this region's nucleotide sequence