Experiment: R2A with rubidium chloride 600 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5428 and Atu5429 are separated by 29 nucleotides Atu5429 and Atu5430 are separated by 415 nucleotides Atu5430 and Atu5431 are separated by 330 nucleotides Atu5431 and Atu5432 are separated by 178 nucleotides
Atu5428: Atu5428 - DNA-damage-inducible protein F, at 422,061 to 423,398
Atu5428
Atu5429: Atu5429 - transcriptional regulator, at 423,428 to 423,595
Atu5429
Atu5430: Atu5430 - hypothetical protein, at 424,011 to 424,433
Atu5430
Atu5431: Atu5431 - hypothetical protein, at 424,764 to 425,153
Atu5431
Atu5432: Atu5432 - hypothetical protein, at 425,332 to 425,640
Atu5432
Position (kb) 424
425 Strain fitness (log2 ratio) -2
-1
0
1
2 at 423.075 kb on + strand, within Atu5428 at 423.103 kb on + strand, within Atu5428 at 423.134 kb on + strand, within Atu5428 at 423.151 kb on - strand, within Atu5428 at 423.236 kb on + strand, within Atu5428 at 423.243 kb on - strand, within Atu5428 at 423.279 kb on + strand at 423.287 kb on - strand at 423.299 kb on - strand at 423.453 kb on + strand, within Atu5429 at 423.498 kb on + strand, within Atu5429 at 423.506 kb on - strand, within Atu5429 at 423.506 kb on - strand, within Atu5429 at 423.511 kb on - strand, within Atu5429 at 423.536 kb on + strand, within Atu5429 at 423.536 kb on + strand, within Atu5429 at 423.538 kb on + strand, within Atu5429 at 423.564 kb on + strand, within Atu5429 at 423.625 kb on - strand at 423.711 kb on - strand at 423.733 kb on - strand at 423.788 kb on + strand at 423.925 kb on - strand at 423.942 kb on + strand at 424.041 kb on + strand at 424.049 kb on - strand at 424.178 kb on - strand, within Atu5430 at 424.287 kb on - strand, within Atu5430 at 424.341 kb on + strand, within Atu5430 at 424.392 kb on + strand at 424.430 kb on + strand at 424.451 kb on - strand at 424.487 kb on - strand at 424.500 kb on + strand at 424.539 kb on - strand at 424.558 kb on - strand at 424.614 kb on + strand at 424.647 kb on + strand at 424.677 kb on - strand at 424.702 kb on - strand at 424.747 kb on - strand at 424.784 kb on + strand at 424.792 kb on - strand at 424.792 kb on - strand at 424.792 kb on - strand at 424.800 kb on - strand at 424.819 kb on + strand, within Atu5431 at 424.870 kb on + strand, within Atu5431 at 424.886 kb on + strand, within Atu5431 at 424.894 kb on - strand, within Atu5431 at 424.894 kb on - strand, within Atu5431 at 424.899 kb on - strand, within Atu5431 at 425.017 kb on - strand, within Atu5431 at 425.030 kb on + strand, within Atu5431 at 425.030 kb on + strand, within Atu5431 at 425.089 kb on + strand, within Atu5431 at 425.094 kb on + strand, within Atu5431 at 425.113 kb on + strand, within Atu5431 at 425.121 kb on - strand at 425.151 kb on - strand at 425.167 kb on - strand at 425.257 kb on + strand at 425.263 kb on + strand at 425.270 kb on + strand at 425.278 kb on - strand at 425.359 kb on + strand at 425.409 kb on + strand, within Atu5432
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with rubidium chloride 600 mM remove 423,075 + Atu5428 0.76 -0.3 423,103 + Atu5428 0.78 +0.1 423,134 + Atu5428 0.80 -0.5 423,151 - Atu5428 0.81 -0.1 423,236 + Atu5428 0.88 +0.6 423,243 - Atu5428 0.88 +0.1 423,279 + +0.1 423,287 - -0.1 423,299 - +1.9 423,453 + Atu5429 0.15 -0.6 423,498 + Atu5429 0.42 -0.7 423,506 - Atu5429 0.46 +1.1 423,506 - Atu5429 0.46 +0.7 423,511 - Atu5429 0.49 -0.4 423,536 + Atu5429 0.64 +0.0 423,536 + Atu5429 0.64 +0.7 423,538 + Atu5429 0.65 -0.4 423,564 + Atu5429 0.81 +0.0 423,625 - -0.4 423,711 - -0.4 423,733 - -0.7 423,788 + +0.2 423,925 - +0.0 423,942 + +1.3 424,041 + +0.3 424,049 - -0.6 424,178 - Atu5430 0.39 -1.7 424,287 - Atu5430 0.65 -0.0 424,341 + Atu5430 0.78 -1.1 424,392 + -0.0 424,430 + +0.2 424,451 - +0.2 424,487 - +0.1 424,500 + -0.2 424,539 - -0.0 424,558 - +0.9 424,614 + +0.6 424,647 + -0.3 424,677 - +0.2 424,702 - -2.3 424,747 - -0.4 424,784 + +0.4 424,792 - +0.5 424,792 - +0.2 424,792 - -0.2 424,800 - -0.2 424,819 + Atu5431 0.14 -0.0 424,870 + Atu5431 0.27 +0.4 424,886 + Atu5431 0.31 +0.0 424,894 - Atu5431 0.33 +0.4 424,894 - Atu5431 0.33 +0.2 424,899 - Atu5431 0.35 -0.1 425,017 - Atu5431 0.65 -0.1 425,030 + Atu5431 0.68 -0.2 425,030 + Atu5431 0.68 -0.0 425,089 + Atu5431 0.83 -0.2 425,094 + Atu5431 0.85 -0.2 425,113 + Atu5431 0.89 +1.1 425,121 - -0.1 425,151 - -0.5 425,167 - -0.3 425,257 + -0.4 425,263 + +0.8 425,270 + +0.2 425,278 - +0.0 425,359 + -1.2 425,409 + Atu5432 0.25 +1.0
Or see this region's nucleotide sequence