Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_2086 and PfGW456L13_2087 are separated by 24 nucleotides PfGW456L13_2087 and PfGW456L13_2088 overlap by 4 nucleotides PfGW456L13_2088 and PfGW456L13_2089 overlap by 1 nucleotides PfGW456L13_2089 and PfGW456L13_2090 overlap by 4 nucleotides
PfGW456L13_2086 - Tetraacyldisaccharide 4'-kinase (EC 2.7.1.130), at 2,292,270 to 2,293,280
_2086
PfGW456L13_2087 - FIG002473: Protein YcaR in KDO2-Lipid A biosynthesis cluster, at 2,293,305 to 2,293,490
_2087
PfGW456L13_2088 - 3-deoxy-manno-octulosonate cytidylyltransferase (EC 2.7.7.38), at 2,293,487 to 2,294,251
_2088
PfGW456L13_2089 - Low molecular weight protein tyrosine phosphatase (EC 3.1.3.48), at 2,294,251 to 2,294,715
_2089
PfGW456L13_2090 - UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.1.1.158), at 2,294,712 to 2,295,731
_2090
Position (kb)
2293
2294
2295 Strain fitness (log2 ratio)
-1
0
1 at 2293.278 kb on + strand at 2294.319 kb on + strand, within PfGW456L13_2089 at 2294.319 kb on + strand, within PfGW456L13_2089 at 2294.491 kb on + strand, within PfGW456L13_2089 at 2294.623 kb on + strand, within PfGW456L13_2089 at 2294.623 kb on + strand, within PfGW456L13_2089
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,293,278 + +0.6 2,294,319 + PfGW456L13_2089 0.15 -1.0 2,294,319 + PfGW456L13_2089 0.15 -1.1 2,294,491 + PfGW456L13_2089 0.52 +1.3 2,294,623 + PfGW456L13_2089 0.80 +1.3 2,294,623 + PfGW456L13_2089 0.80 +0.5
Or see this region's nucleotide sequence