Strain Fitness in Agrobacterium fabrum C58 around Atu1112

Experiment: R2A with Strontium chloride 750 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAtu1110 and Atu1111 are separated by 25 nucleotidesAtu1111 and Atu1112 are separated by 154 nucleotidesAtu1112 and Atu1113 are separated by 30 nucleotides Atu1110: Atu1110 - cytosol aminopeptidase, at 1,099,854 to 1,101,347 Atu1110 Atu1111: Atu1111 - DNA polymerase III subunit chi, at 1,101,373 to 1,101,822 Atu1111 Atu1112: Atu1112 - hypothetical protein, at 1,101,977 to 1,103,005 Atu1112 Atu1113: Atu1113 - dehydrogenase, at 1,103,036 to 1,103,962 Atu1113 Position (kb) 1101 1102 1103 1104Strain fitness (log2 ratio) -2 -1 0 1at 1100.994 kb on + strand, within Atu1110at 1101.144 kb on + strand, within Atu1110at 1101.171 kb on + strand, within Atu1110at 1101.252 kb on - strandat 1101.289 kb on - strandat 1101.289 kb on - strandat 1101.301 kb on + strandat 1101.833 kb on + strandat 1101.841 kb on - strandat 1101.841 kb on - strandat 1101.841 kb on - strandat 1101.863 kb on - strandat 1101.943 kb on - strandat 1101.980 kb on + strandat 1101.998 kb on - strandat 1102.080 kb on + strand, within Atu1112at 1102.090 kb on + strand, within Atu1112at 1102.245 kb on + strand, within Atu1112at 1102.289 kb on + strand, within Atu1112at 1102.370 kb on - strand, within Atu1112at 1102.370 kb on - strand, within Atu1112at 1102.377 kb on + strand, within Atu1112at 1102.385 kb on - strand, within Atu1112at 1102.385 kb on - strand, within Atu1112at 1102.476 kb on + strand, within Atu1112at 1102.488 kb on - strand, within Atu1112at 1102.543 kb on + strand, within Atu1112at 1102.558 kb on - strand, within Atu1112at 1102.570 kb on + strand, within Atu1112at 1102.660 kb on + strand, within Atu1112at 1102.678 kb on - strand, within Atu1112at 1102.855 kb on + strand, within Atu1112at 1102.857 kb on + strand, within Atu1112at 1102.928 kb on + strandat 1102.928 kb on + strandat 1102.936 kb on - strandat 1103.008 kb on - strandat 1103.013 kb on + strandat 1103.023 kb on + strandat 1103.130 kb on + strand, within Atu1113at 1103.279 kb on + strand, within Atu1113at 1103.297 kb on - strand, within Atu1113at 1103.399 kb on + strand, within Atu1113at 1103.402 kb on + strand, within Atu1113at 1103.408 kb on - strand, within Atu1113at 1103.408 kb on - strand, within Atu1113at 1103.408 kb on - strand, within Atu1113at 1103.422 kb on + strand, within Atu1113at 1103.435 kb on + strand, within Atu1113at 1103.503 kb on - strand, within Atu1113at 1103.557 kb on - strand, within Atu1113at 1103.870 kb on - strandat 1103.870 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Strontium chloride 750 mM
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1,100,994 + Atu1110 0.76 -1.2
1,101,144 + Atu1110 0.86 -0.1
1,101,171 + Atu1110 0.88 +0.3
1,101,252 - -1.2
1,101,289 - -0.6
1,101,289 - -1.3
1,101,301 + -0.8
1,101,833 + -0.2
1,101,841 - +0.1
1,101,841 - +0.9
1,101,841 - +0.5
1,101,863 - -1.4
1,101,943 - +0.5
1,101,980 + -0.1
1,101,998 - -0.4
1,102,080 + Atu1112 0.10 -1.4
1,102,090 + Atu1112 0.11 +0.1
1,102,245 + Atu1112 0.26 -0.6
1,102,289 + Atu1112 0.30 +0.3
1,102,370 - Atu1112 0.38 -0.1
1,102,370 - Atu1112 0.38 -1.4
1,102,377 + Atu1112 0.39 -0.4
1,102,385 - Atu1112 0.40 +0.2
1,102,385 - Atu1112 0.40 +0.3
1,102,476 + Atu1112 0.48 -0.3
1,102,488 - Atu1112 0.50 -0.3
1,102,543 + Atu1112 0.55 -1.4
1,102,558 - Atu1112 0.56 -0.3
1,102,570 + Atu1112 0.58 -0.7
1,102,660 + Atu1112 0.66 -0.4
1,102,678 - Atu1112 0.68 -0.5
1,102,855 + Atu1112 0.85 +0.2
1,102,857 + Atu1112 0.86 -0.4
1,102,928 + -0.3
1,102,928 + -0.7
1,102,936 - +0.1
1,103,008 - +0.3
1,103,013 + -0.0
1,103,023 + +0.2
1,103,130 + Atu1113 0.10 -0.1
1,103,279 + Atu1113 0.26 +0.2
1,103,297 - Atu1113 0.28 -0.1
1,103,399 + Atu1113 0.39 +0.6
1,103,402 + Atu1113 0.39 -2.0
1,103,408 - Atu1113 0.40 +0.6
1,103,408 - Atu1113 0.40 -1.0
1,103,408 - Atu1113 0.40 -0.1
1,103,422 + Atu1113 0.42 -0.4
1,103,435 + Atu1113 0.43 +0.2
1,103,503 - Atu1113 0.50 +0.3
1,103,557 - Atu1113 0.56 -0.2
1,103,870 - +0.7
1,103,870 - -0.1

Or see this region's nucleotide sequence