Experiment: R2A with Chloride 800 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5424 and Atu5425 are separated by 10 nucleotides Atu5425 and Atu5426 are separated by 7 nucleotides Atu5426 and Atu5427 are separated by 1 nucleotides Atu5427 and Atu5428 are separated by 0 nucleotides
Atu5424: Atu5424 - oxidoreductase, at 418,884 to 420,362
Atu5424
Atu5425: Atu5425 - 2-hydroxychromene-2-carboxylate isomerase, at 420,373 to 420,966
Atu5425
Atu5426: Atu5426 - hypothetical protein, at 420,974 to 421,480
Atu5426
Atu5427: Atu5427 - hypothetical protein, at 421,482 to 422,060
Atu5427
Atu5428: Atu5428 - DNA-damage-inducible protein F, at 422,061 to 423,398
Atu5428
Position (kb) 420
421
422 Strain fitness (log2 ratio) -1
0
1
2
3 at 419.997 kb on + strand, within Atu5424 at 420.020 kb on + strand, within Atu5424 at 420.020 kb on + strand, within Atu5424 at 420.020 kb on + strand, within Atu5424 at 420.020 kb on + strand, within Atu5424 at 420.020 kb on + strand, within Atu5424 at 420.020 kb on + strand, within Atu5424 at 420.028 kb on - strand, within Atu5424 at 420.032 kb on + strand, within Atu5424 at 420.032 kb on + strand, within Atu5424 at 420.050 kb on + strand, within Atu5424 at 420.129 kb on + strand, within Atu5424 at 420.220 kb on - strand at 420.221 kb on + strand at 420.266 kb on + strand at 420.274 kb on - strand at 420.277 kb on + strand at 420.318 kb on - strand at 420.318 kb on - strand at 420.318 kb on - strand at 420.331 kb on + strand at 420.347 kb on + strand at 420.365 kb on - strand at 420.371 kb on - strand at 420.423 kb on - strand at 420.427 kb on - strand at 420.449 kb on - strand, within Atu5425 at 420.475 kb on - strand, within Atu5425 at 420.535 kb on + strand, within Atu5425 at 420.549 kb on + strand, within Atu5425 at 420.675 kb on + strand, within Atu5425 at 420.683 kb on - strand, within Atu5425 at 420.686 kb on + strand, within Atu5425 at 420.706 kb on - strand, within Atu5425 at 420.730 kb on - strand, within Atu5425 at 420.743 kb on - strand, within Atu5425 at 420.799 kb on - strand, within Atu5425 at 420.799 kb on - strand, within Atu5425 at 420.816 kb on - strand, within Atu5425 at 420.841 kb on + strand, within Atu5425 at 420.852 kb on + strand, within Atu5425 at 420.919 kb on + strand at 420.922 kb on - strand at 420.927 kb on - strand at 420.986 kb on + strand at 421.025 kb on - strand, within Atu5426 at 421.029 kb on + strand, within Atu5426 at 421.074 kb on - strand, within Atu5426 at 421.118 kb on - strand, within Atu5426 at 421.143 kb on + strand, within Atu5426 at 421.143 kb on + strand, within Atu5426 at 421.201 kb on - strand, within Atu5426 at 421.222 kb on + strand, within Atu5426 at 421.222 kb on + strand, within Atu5426 at 421.366 kb on + strand, within Atu5426 at 421.382 kb on - strand, within Atu5426 at 421.438 kb on + strand at 421.581 kb on + strand, within Atu5427 at 421.607 kb on - strand, within Atu5427 at 421.610 kb on + strand, within Atu5427 at 421.663 kb on - strand, within Atu5427 at 421.757 kb on - strand, within Atu5427 at 421.787 kb on + strand, within Atu5427 at 422.009 kb on - strand at 422.034 kb on - strand at 422.039 kb on - strand at 422.068 kb on - strand at 422.078 kb on + strand at 422.174 kb on + strand at 422.182 kb on + strand at 422.236 kb on + strand, within Atu5428 at 422.241 kb on + strand, within Atu5428 at 422.244 kb on - strand, within Atu5428 at 422.244 kb on - strand, within Atu5428 at 422.246 kb on + strand, within Atu5428 at 422.249 kb on - strand, within Atu5428 at 422.291 kb on + strand, within Atu5428 at 422.426 kb on - strand, within Atu5428
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 800 mM remove 419,997 + Atu5424 0.75 -0.1 420,020 + Atu5424 0.77 +0.5 420,020 + Atu5424 0.77 +0.0 420,020 + Atu5424 0.77 -1.4 420,020 + Atu5424 0.77 +2.0 420,020 + Atu5424 0.77 -0.4 420,020 + Atu5424 0.77 -1.0 420,028 - Atu5424 0.77 -1.1 420,032 + Atu5424 0.78 -0.7 420,032 + Atu5424 0.78 -0.3 420,050 + Atu5424 0.79 -0.8 420,129 + Atu5424 0.84 -0.2 420,220 - -0.4 420,221 + +0.5 420,266 + -0.2 420,274 - +0.4 420,277 + -1.2 420,318 - -1.4 420,318 - +0.1 420,318 - -0.7 420,331 + -0.4 420,347 + -0.4 420,365 - -0.0 420,371 - -0.1 420,423 - +1.7 420,427 - +1.8 420,449 - Atu5425 0.13 +1.4 420,475 - Atu5425 0.17 +2.7 420,535 + Atu5425 0.27 +1.3 420,549 + Atu5425 0.30 +0.8 420,675 + Atu5425 0.51 +0.3 420,683 - Atu5425 0.52 +0.0 420,686 + Atu5425 0.53 +0.6 420,706 - Atu5425 0.56 +0.3 420,730 - Atu5425 0.60 +0.3 420,743 - Atu5425 0.62 -0.1 420,799 - Atu5425 0.72 -0.3 420,799 - Atu5425 0.72 +0.3 420,816 - Atu5425 0.75 +0.2 420,841 + Atu5425 0.79 -0.0 420,852 + Atu5425 0.81 -0.4 420,919 + +0.3 420,922 - +0.5 420,927 - +1.5 420,986 + +2.1 421,025 - Atu5426 0.10 +2.3 421,029 + Atu5426 0.11 +2.3 421,074 - Atu5426 0.20 +2.1 421,118 - Atu5426 0.28 +3.4 421,143 + Atu5426 0.33 +2.4 421,143 + Atu5426 0.33 +2.2 421,201 - Atu5426 0.45 +2.6 421,222 + Atu5426 0.49 +2.1 421,222 + Atu5426 0.49 +2.1 421,366 + Atu5426 0.77 +1.4 421,382 - Atu5426 0.80 +1.8 421,438 + +1.9 421,581 + Atu5427 0.17 +1.7 421,607 - Atu5427 0.22 +2.6 421,610 + Atu5427 0.22 +2.1 421,663 - Atu5427 0.31 +1.9 421,757 - Atu5427 0.47 +2.0 421,787 + Atu5427 0.53 +2.3 422,009 - +2.1 422,034 - +2.4 422,039 - +1.9 422,068 - +0.0 422,078 + -0.3 422,174 + -0.4 422,182 + +1.7 422,236 + Atu5428 0.13 -0.1 422,241 + Atu5428 0.13 -1.3 422,244 - Atu5428 0.14 +0.3 422,244 - Atu5428 0.14 -0.3 422,246 + Atu5428 0.14 +0.2 422,249 - Atu5428 0.14 +1.7 422,291 + Atu5428 0.17 +0.7 422,426 - Atu5428 0.27 +1.4
Or see this region's nucleotide sequence