Experiment: R2A with Chloride 800 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5421 and Atu5422 are separated by 9 nucleotides Atu5422 and Atu5423 are separated by 3 nucleotides Atu5423 and Atu5424 are separated by 60 nucleotides
Atu5421: Atu5421 - ABC transporter membrane spanning protein (putrescine), at 415,965 to 416,948
Atu5421
Atu5422: Atu5422 - ABC transporter membrane spanning protein (putrescine), at 416,958 to 417,749
Atu5422
Atu5423: Atu5423 - ABC transporter nucleotide binding/ATPase (putrescine), at 417,753 to 418,823
Atu5423
Atu5424: Atu5424 - oxidoreductase, at 418,884 to 420,362
Atu5424
Position (kb) 417
418
419 Strain fitness (log2 ratio) -2
-1
0
1 at 416.774 kb on - strand, within Atu5421 at 416.820 kb on + strand, within Atu5421 at 416.838 kb on - strand, within Atu5421 at 416.846 kb on + strand, within Atu5421 at 416.867 kb on - strand at 416.951 kb on - strand at 416.975 kb on - strand at 417.012 kb on - strand at 417.061 kb on + strand, within Atu5422 at 417.069 kb on - strand, within Atu5422 at 417.124 kb on - strand, within Atu5422 at 417.305 kb on + strand, within Atu5422 at 417.313 kb on - strand, within Atu5422 at 417.350 kb on - strand, within Atu5422 at 417.377 kb on + strand, within Atu5422 at 417.377 kb on + strand, within Atu5422 at 417.377 kb on + strand, within Atu5422 at 417.385 kb on - strand, within Atu5422 at 417.385 kb on - strand, within Atu5422 at 417.425 kb on - strand, within Atu5422 at 417.425 kb on - strand, within Atu5422 at 417.433 kb on + strand, within Atu5422 at 417.520 kb on - strand, within Atu5422 at 417.520 kb on - strand, within Atu5422 at 417.565 kb on + strand, within Atu5422 at 417.586 kb on + strand, within Atu5422 at 417.586 kb on + strand, within Atu5422 at 417.594 kb on - strand, within Atu5422 at 417.594 kb on - strand, within Atu5422 at 417.621 kb on + strand, within Atu5422 at 417.662 kb on + strand, within Atu5422 at 417.675 kb on - strand at 417.723 kb on - strand at 417.756 kb on - strand at 417.756 kb on - strand at 417.788 kb on + strand at 417.802 kb on + strand at 417.824 kb on + strand at 417.825 kb on - strand at 417.847 kb on + strand at 417.873 kb on - strand, within Atu5423 at 417.899 kb on + strand, within Atu5423 at 417.976 kb on + strand, within Atu5423 at 417.976 kb on + strand, within Atu5423 at 418.032 kb on - strand, within Atu5423 at 418.088 kb on + strand, within Atu5423 at 418.160 kb on - strand, within Atu5423 at 418.187 kb on + strand, within Atu5423 at 418.249 kb on + strand, within Atu5423 at 418.250 kb on - strand, within Atu5423 at 418.397 kb on + strand, within Atu5423 at 418.400 kb on - strand, within Atu5423 at 418.410 kb on + strand, within Atu5423 at 418.507 kb on - strand, within Atu5423 at 418.567 kb on - strand, within Atu5423 at 418.567 kb on - strand, within Atu5423 at 418.595 kb on + strand, within Atu5423 at 418.603 kb on - strand, within Atu5423 at 418.613 kb on - strand, within Atu5423 at 418.673 kb on + strand, within Atu5423 at 418.673 kb on + strand, within Atu5423 at 418.681 kb on - strand, within Atu5423 at 418.784 kb on + strand at 418.804 kb on + strand at 418.804 kb on + strand at 418.817 kb on - strand at 418.838 kb on + strand at 418.904 kb on - strand at 418.904 kb on - strand at 418.911 kb on + strand at 418.940 kb on + strand at 418.948 kb on - strand at 418.974 kb on + strand at 418.977 kb on - strand at 418.984 kb on + strand at 418.984 kb on + strand at 418.994 kb on + strand at 418.994 kb on + strand at 418.994 kb on + strand at 419.167 kb on - strand, within Atu5424 at 419.178 kb on + strand, within Atu5424 at 419.194 kb on + strand, within Atu5424 at 419.288 kb on + strand, within Atu5424 at 419.296 kb on - strand, within Atu5424 at 419.296 kb on - strand, within Atu5424 at 419.315 kb on + strand, within Atu5424 at 419.459 kb on + strand, within Atu5424 at 419.474 kb on - strand, within Atu5424 at 419.501 kb on + strand, within Atu5424 at 419.587 kb on + strand, within Atu5424 at 419.589 kb on + strand, within Atu5424 at 419.606 kb on + strand, within Atu5424 at 419.671 kb on - strand, within Atu5424 at 419.773 kb on - strand, within Atu5424 at 419.793 kb on + strand, within Atu5424 at 419.801 kb on + strand, within Atu5424 at 419.803 kb on + strand, within Atu5424
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 800 mM remove 416,774 - Atu5421 0.82 +0.6 416,820 + Atu5421 0.87 +0.4 416,838 - Atu5421 0.89 -0.4 416,846 + Atu5421 0.90 +0.8 416,867 - -0.6 416,951 - +0.0 416,975 - -0.4 417,012 - -0.6 417,061 + Atu5422 0.13 -0.5 417,069 - Atu5422 0.14 +0.7 417,124 - Atu5422 0.21 -0.8 417,305 + Atu5422 0.44 -0.7 417,313 - Atu5422 0.45 -0.4 417,350 - Atu5422 0.49 -0.7 417,377 + Atu5422 0.53 -0.0 417,377 + Atu5422 0.53 +0.1 417,377 + Atu5422 0.53 -1.8 417,385 - Atu5422 0.54 -1.1 417,385 - Atu5422 0.54 -1.5 417,425 - Atu5422 0.59 -0.9 417,425 - Atu5422 0.59 +1.0 417,433 + Atu5422 0.60 -0.1 417,520 - Atu5422 0.71 +0.9 417,520 - Atu5422 0.71 +0.2 417,565 + Atu5422 0.77 +0.1 417,586 + Atu5422 0.79 -0.2 417,586 + Atu5422 0.79 -0.2 417,594 - Atu5422 0.80 -0.0 417,594 - Atu5422 0.80 -0.8 417,621 + Atu5422 0.84 +1.0 417,662 + Atu5422 0.89 +0.1 417,675 - -0.3 417,723 - +0.1 417,756 - -0.4 417,756 - -0.3 417,788 + -0.3 417,802 + -0.5 417,824 + +0.0 417,825 - -0.3 417,847 + -0.6 417,873 - Atu5423 0.11 -0.0 417,899 + Atu5423 0.14 +0.4 417,976 + Atu5423 0.21 -1.6 417,976 + Atu5423 0.21 -0.5 418,032 - Atu5423 0.26 -1.1 418,088 + Atu5423 0.31 +0.1 418,160 - Atu5423 0.38 +0.0 418,187 + Atu5423 0.41 -0.1 418,249 + Atu5423 0.46 +1.6 418,250 - Atu5423 0.46 -0.3 418,397 + Atu5423 0.60 -0.8 418,400 - Atu5423 0.60 -0.5 418,410 + Atu5423 0.61 -1.0 418,507 - Atu5423 0.70 -0.4 418,567 - Atu5423 0.76 -0.4 418,567 - Atu5423 0.76 +1.3 418,595 + Atu5423 0.79 +0.3 418,603 - Atu5423 0.79 -1.7 418,613 - Atu5423 0.80 +0.4 418,673 + Atu5423 0.86 -0.1 418,673 + Atu5423 0.86 -0.9 418,681 - Atu5423 0.87 -2.2 418,784 + -0.2 418,804 + -1.0 418,804 + -1.1 418,817 - -0.7 418,838 + -1.4 418,904 - -0.6 418,904 - +0.6 418,911 + -0.1 418,940 + -0.9 418,948 - -0.1 418,974 + -0.8 418,977 - -0.9 418,984 + -1.6 418,984 + +0.8 418,994 + -0.2 418,994 + -0.0 418,994 + -0.7 419,167 - Atu5424 0.19 -0.5 419,178 + Atu5424 0.20 -0.1 419,194 + Atu5424 0.21 -0.0 419,288 + Atu5424 0.27 -0.1 419,296 - Atu5424 0.28 -1.0 419,296 - Atu5424 0.28 +1.6 419,315 + Atu5424 0.29 +0.0 419,459 + Atu5424 0.39 -0.3 419,474 - Atu5424 0.40 -0.3 419,501 + Atu5424 0.42 -0.2 419,587 + Atu5424 0.48 -0.0 419,589 + Atu5424 0.48 +0.1 419,606 + Atu5424 0.49 -0.6 419,671 - Atu5424 0.53 -0.4 419,773 - Atu5424 0.60 -0.0 419,793 + Atu5424 0.61 +0.5 419,801 + Atu5424 0.62 -0.2 419,803 + Atu5424 0.62 -0.9
Or see this region's nucleotide sequence